Fast induction of nisin resistance in Streptococcus thermophilus INIA 463 during growth in milk
Streptococcus thermophilus INIA 463 became nisin-resistant after exposure in skim milk to subminimal inhibitory concentrations of nisin (1-3 IU/ml) for less than 2 h. Addition of 20 IU/ml caused a 4 log unit decrease in S. thermophilus counts of a culture not exposed previously to nisin, whereas no decrease was observed in the culture exposed to nisin for 2 h. Transfer of immunity genes as responsible for nisin resistance was discarded. The presence of extracellular or intracellular specific nisin-degrading enzymes was not detected in the nisin-resistant variant of S. thermophilus INIA 463. Nisin resistance was caused by the induction of a resistance mechanism. Transmission electron microscopy (TEM) revealed that the nisin-resistant variant of S. thermophilus INIA 463 had a thickened cell wall compared to the wild strain. Resistance to nisin was lost after one transfer (4 h growth) in nisin-free skim milk. © 2004 Elsevier B.V. All rights reserved.
Main Authors: | Garde López-Brea, Sonia, Ávila Arribas, Marta, Medina, M., Núñez Gutiérrez, Manuel |
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Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2004
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Subjects: | Streptococcus thermophilus, Nisin resistance, Inducible mechanism, Cell wall, |
Online Access: | http://hdl.handle.net/20.500.12792/1664 http://hdl.handle.net/10261/292944 |
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