The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality
In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2015
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Subjects: | Embryo quality, Follicular fluid, In-vitro maturation, IVF, Oocyte developmental competence, |
Online Access: | http://hdl.handle.net/20.500.12792/4608 http://hdl.handle.net/10261/292343 |
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dig-inia-es-10261-2923432023-02-20T07:27:58Z The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality Valckx, S. D. M. De Bie, J. Michiels, E. D. Goovaerts, I. G. Punjabi, U. Ramos Ibeas, Priscila Gutiérrez Adán, Alfonso Bols, P. E. Leroy, J. L. Embryo quality Follicular fluid In-vitro maturation IVF Oocyte developmental competence In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. 2023-02-20T07:27:58Z 2023-02-20T07:27:58Z 2015 artículo Reproductive BioMedicine Online 30(2): 203-207 (2015) 1472-6483 http://hdl.handle.net/20.500.12792/4608 http://hdl.handle.net/10261/292343 10.1016/j.rbmo.2014.10.008 en none Elsevier |
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Embryo quality Follicular fluid In-vitro maturation IVF Oocyte developmental competence Embryo quality Follicular fluid In-vitro maturation IVF Oocyte developmental competence |
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Embryo quality Follicular fluid In-vitro maturation IVF Oocyte developmental competence Embryo quality Follicular fluid In-vitro maturation IVF Oocyte developmental competence Valckx, S. D. M. De Bie, J. Michiels, E. D. Goovaerts, I. G. Punjabi, U. Ramos Ibeas, Priscila Gutiérrez Adán, Alfonso Bols, P. E. Leroy, J. L. The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
description |
In this study, the hypothesis that embryo development during routine IVF procedures is determined by the pre-ovulatory follicular fluid composition was tested. Follicular fluid from women with obesity ('obese') and a 'positive' or 'negative' IVF outcome was added during the in-vitro maturation of bovine oocytes. 'Negative' and 'obese' follicular fluid reduced bovine embryo development, compared with laboratory control embryo development (P < 0.05 or P < 0.1). The addition of follicular fluid also altered bovine blastocyst gene expression. Furthermore, LDHA and PPARGC1B gene expression differed between follicular fluid groups. Data suggest that pre-ovulatory follicular fluid can potentially affect oocyte developmental competence and embryo quality. Furthermore, the bovine model may be used as a screening tool. © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. |
format |
artículo |
topic_facet |
Embryo quality Follicular fluid In-vitro maturation IVF Oocyte developmental competence |
author |
Valckx, S. D. M. De Bie, J. Michiels, E. D. Goovaerts, I. G. Punjabi, U. Ramos Ibeas, Priscila Gutiérrez Adán, Alfonso Bols, P. E. Leroy, J. L. |
author_facet |
Valckx, S. D. M. De Bie, J. Michiels, E. D. Goovaerts, I. G. Punjabi, U. Ramos Ibeas, Priscila Gutiérrez Adán, Alfonso Bols, P. E. Leroy, J. L. |
author_sort |
Valckx, S. D. M. |
title |
The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
title_short |
The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
title_full |
The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
title_fullStr |
The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
title_full_unstemmed |
The effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
title_sort |
effect of human follicular fluid on bovine oocyte developmental competence and embryo quality |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/20.500.12792/4608 http://hdl.handle.net/10261/292343 |
work_keys_str_mv |
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