Growth stimulation of a proteinase positive Lactococcus lactis strain by a proteinase negative Lactococcus lactis strain

Lactococcus lactis AMP15/pAMP31(D471R) is a proteinase negative, lactose negative strain with a modified oligopeptide transport system, and potential as a debittering agent due to its efficient utilization of hydrophobic peptides. Five wild L. lactis strains of dairy origin, which produced cheeses of high flavour quality, were cocultured with L. lactis AMP15/pAMP31(D471R) in an attempt to select adequate combinations of strains for use as defined cheese starters with potential debittering ability. Four of these strains, L. lactis B6, K16, M21 and P21, inhibited growth of L. lactis AMP15/pAMP31(D471R) at a level of 106 to 107 cfu mL- 1 after 24 h of incubation, even though production of bacteriocin-like compounds could only be proven for L. lactis M21. When L. lactis AMP15/pAMP31(D471R) was cocultured with the fifth strain, L. lactis N22, its growth was significantly (P < 0.001) inhibited whereas growth of L. lactis N22 was significantly stimulated. The nature of the interaction was studied and it was established that L. lactis N22 is auxotrophic for folate, a compound produced and excreted by L. lactis AMP15/pAMP31(D471R). © 2007 Elsevier B.V. All rights reserved.

Bibliographic Details

Main Authors:	Picón Gálvez, Antonia María, Núñez Gutiérrez, Manuel
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2007
Subjects:	Lactic starters, Oligopeptide binding protein, Cocultures, Folate,
Online Access:	http://hdl.handle.net/20.500.12792/3520 http://hdl.handle.net/10261/291663
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