The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro
Here we examined whether the addition of a non-toxic concentration (6.25. μg/mL) of zinc oxide nanoparticles (ZnONPs 19, 35 and 57. nm, respectively) modulates the cytotoxicity of copper nanoparticles (CuNPs, 63. nm in size) in the human hepatoma cell line HepG2. The cytotoxic effect of CuNPs on HepG2 cells was markedly enhanced by the ZnONPs, the largest ZnONPs causing the highest increase in toxicity. However, CuNPs cytotoxicity was not affected by co-incubation with medium containing only zinc ions, indicating the increase in toxicity might be attributed to the particle form of ZnONPs. Transmission electron microscopy (TEM) revealed the presence of CuNPs and ZnONPs inside the cells co-exposed to both types of NP and outflow of cytoplasm through the damaged cell membrane. Inductively coupled plasma mass spectrometry (ICP-MS) determined an increase in the concentration of zinc and a decrease in that of copper in co-exposed cells. On the basis of these results, we propose that accumulation of large numbers of ZnONPs in the cells alters cellular membranes and the cytotoxicity of CuNPs is increased. © 2014 Elsevier B.V.
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Language: | English |
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Elsevier
2015
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Subjects: | ZnONPs, CuNPs, HepG2, Potentiation effect, Cytotoxicity assays, |
Online Access: | http://hdl.handle.net/10261/291562 |
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dig-inia-es-10261-2915622023-06-16T10:44:18Z The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro Li, L. Fernández Cruz, María Luisa Connoly, Mona Conde, E. Fernández Cruz, María Luisa Schuster, M. Navas Antón, José María Connoly, Mona [0000-0003-3574-8564] ZnONPs CuNPs HepG2 Potentiation effect Cytotoxicity assays Here we examined whether the addition of a non-toxic concentration (6.25. μg/mL) of zinc oxide nanoparticles (ZnONPs 19, 35 and 57. nm, respectively) modulates the cytotoxicity of copper nanoparticles (CuNPs, 63. nm in size) in the human hepatoma cell line HepG2. The cytotoxic effect of CuNPs on HepG2 cells was markedly enhanced by the ZnONPs, the largest ZnONPs causing the highest increase in toxicity. However, CuNPs cytotoxicity was not affected by co-incubation with medium containing only zinc ions, indicating the increase in toxicity might be attributed to the particle form of ZnONPs. Transmission electron microscopy (TEM) revealed the presence of CuNPs and ZnONPs inside the cells co-exposed to both types of NP and outflow of cytoplasm through the damaged cell membrane. Inductively coupled plasma mass spectrometry (ICP-MS) determined an increase in the concentration of zinc and a decrease in that of copper in co-exposed cells. On the basis of these results, we propose that accumulation of large numbers of ZnONPs in the cells alters cellular membranes and the cytotoxicity of CuNPs is increased. © 2014 Elsevier B.V. 2023-02-20T07:19:30Z 2023-02-20T07:19:30Z 2015 artículo http://purl.org/coar/resource_type/c_6501 Science of the Total Environment 505: 253-260 (2015) 0048-9697 http://hdl.handle.net/10261/291562 10.1016/j.scitotenv.2014.10.020 1879-1026 en Departamento de Medio Ambiente y Agronomía Sí none Elsevier |
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ZnONPs CuNPs HepG2 Potentiation effect Cytotoxicity assays ZnONPs CuNPs HepG2 Potentiation effect Cytotoxicity assays |
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ZnONPs CuNPs HepG2 Potentiation effect Cytotoxicity assays ZnONPs CuNPs HepG2 Potentiation effect Cytotoxicity assays Li, L. Fernández Cruz, María Luisa Connoly, Mona Conde, E. Fernández Cruz, María Luisa Schuster, M. Navas Antón, José María The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro |
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Here we examined whether the addition of a non-toxic concentration (6.25. μg/mL) of zinc oxide nanoparticles (ZnONPs 19, 35 and 57. nm, respectively) modulates the cytotoxicity of copper nanoparticles (CuNPs, 63. nm in size) in the human hepatoma cell line HepG2. The cytotoxic effect of CuNPs on HepG2 cells was markedly enhanced by the ZnONPs, the largest ZnONPs causing the highest increase in toxicity. However, CuNPs cytotoxicity was not affected by co-incubation with medium containing only zinc ions, indicating the increase in toxicity might be attributed to the particle form of ZnONPs. Transmission electron microscopy (TEM) revealed the presence of CuNPs and ZnONPs inside the cells co-exposed to both types of NP and outflow of cytoplasm through the damaged cell membrane. Inductively coupled plasma mass spectrometry (ICP-MS) determined an increase in the concentration of zinc and a decrease in that of copper in co-exposed cells. On the basis of these results, we propose that accumulation of large numbers of ZnONPs in the cells alters cellular membranes and the cytotoxicity of CuNPs is increased. © 2014 Elsevier B.V. |
author2 |
Connoly, Mona [0000-0003-3574-8564] |
author_facet |
Connoly, Mona [0000-0003-3574-8564] Li, L. Fernández Cruz, María Luisa Connoly, Mona Conde, E. Fernández Cruz, María Luisa Schuster, M. Navas Antón, José María |
format |
artículo |
topic_facet |
ZnONPs CuNPs HepG2 Potentiation effect Cytotoxicity assays |
author |
Li, L. Fernández Cruz, María Luisa Connoly, Mona Conde, E. Fernández Cruz, María Luisa Schuster, M. Navas Antón, José María |
author_sort |
Li, L. |
title |
The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro |
title_short |
The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro |
title_full |
The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro |
title_fullStr |
The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro |
title_full_unstemmed |
The potentiation effect makes the difference Non-toxic concentrations of ZnO nanoparticles enhance Cu nanoparticle toxicity in vitro |
title_sort |
potentiation effect makes the difference non-toxic concentrations of zno nanoparticles enhance cu nanoparticle toxicity in vitro |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/10261/291562 |
work_keys_str_mv |
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