Influence of a modified peptide transport system in Lactococcus lactis on the utilization of peptides formed by chymosin and milk proteinases
Oligopeptides present in cheese are transported by Lactococcus lactis strains, via the oligopeptide transport system (Opp), to the inside of the cell where they are degraded to free amino acids. Prt- and Lac- L. lactis strains (MG1363 ΔoppA derivatives) expressing a wild-type MG1363 substrate binding protein (OppA) or a mutant OppA with a single point mutation at residue 471 (OppA(D471R)) from a plasmid were used in cheesemaking trials to elucidate if differences in peptide transport system could result in different peptide contents in cheese. Cheeses made with the L. lactis strain expressing OppA(D471R) had lower levels of hydrophilic and hydrophobic peptides than cheeses made with the L. lactis strain expressing wild-type OppA, pointing to a more efficient peptide utilization by the former strain.
| Main Authors: | , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
AVA Agrar-Verlag Allgäu
2006
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| Online Access: | http://hdl.handle.net/20.500.12792/5394 http://hdl.handle.net/10261/291021 |
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