Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus
Rigid amphipathic fusion inhibitors (RAFIs) are a family of nucleoside derivatives that inhibit the infectivity of several enveloped viruses by interacting with virion envelope lipids and inhibiting fusion between viral and cellular membranes. Here we tested the antiviral activity of two RAFIs, 5-(Perylen-3-ylethynyl)-arabino-uridine (aUY11) and 5-(Perylen-3-ylethynyl)uracil-1-acetic acid (cm1UY11) against African swine fever virus (ASFV), for which no effective vaccine is available. Both compounds displayed a potent, dose-dependent inhibitory effect on ASFV infection in Vero cells. The major antiviral effect was observed when aUY11 and cm1UY11 were added at early stages of infection and maintained during the complete viral cycle. Furthermore, virucidal assay revealed a significant extracellular anti-ASFV activity for both compounds. We also found decrease in the synthesis of early and late viral proteins in Vero cells treated with cm1UY11. Finally, the inhibitory effect of aUY11 and cm1UY11 on ASFV infection in porcine alveolar macrophages was confirmed. Overall, our study has identified novel anti-ASFV compounds with potential for future therapeutic developments.
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| Language: | English |
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Microbiology Society
2018
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| Subjects: | African swine fever virus, Antivirals, Antiviral therapy, Nucleoside analogues, |
| Online Access: | http://hdl.handle.net/20.500.12792/564 http://hdl.handle.net/10261/290732 |
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dig-inia-es-10261-2907322023-02-17T12:28:50Z Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus Hakobyan, A. Galindo Barreales, Inmaculada Nañez, A. Arabyan, E. Karalyan, Z. Chistov, A. A. Streshnev, P. P. Korshun, V. A. Alonso, C. Zakaryan, H. African swine fever virus Antivirals Antiviral therapy Nucleoside analogues Rigid amphipathic fusion inhibitors (RAFIs) are a family of nucleoside derivatives that inhibit the infectivity of several enveloped viruses by interacting with virion envelope lipids and inhibiting fusion between viral and cellular membranes. Here we tested the antiviral activity of two RAFIs, 5-(Perylen-3-ylethynyl)-arabino-uridine (aUY11) and 5-(Perylen-3-ylethynyl)uracil-1-acetic acid (cm1UY11) against African swine fever virus (ASFV), for which no effective vaccine is available. Both compounds displayed a potent, dose-dependent inhibitory effect on ASFV infection in Vero cells. The major antiviral effect was observed when aUY11 and cm1UY11 were added at early stages of infection and maintained during the complete viral cycle. Furthermore, virucidal assay revealed a significant extracellular anti-ASFV activity for both compounds. We also found decrease in the synthesis of early and late viral proteins in Vero cells treated with cm1UY11. Finally, the inhibitory effect of aUY11 and cm1UY11 on ASFV infection in porcine alveolar macrophages was confirmed. Overall, our study has identified novel anti-ASFV compounds with potential for future therapeutic developments. 2023-02-17T12:28:50Z 2023-02-17T12:28:50Z 2018 artículo Journal of General Virology 99: 148-156 (2018) 0022-1317 http://hdl.handle.net/20.500.12792/564 http://hdl.handle.net/10261/290732 10.1099/jgv.0.000991 1465-2099 en none Microbiology Society |
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African swine fever virus Antivirals Antiviral therapy Nucleoside analogues African swine fever virus Antivirals Antiviral therapy Nucleoside analogues |
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African swine fever virus Antivirals Antiviral therapy Nucleoside analogues African swine fever virus Antivirals Antiviral therapy Nucleoside analogues Hakobyan, A. Galindo Barreales, Inmaculada Nañez, A. Arabyan, E. Karalyan, Z. Chistov, A. A. Streshnev, P. P. Korshun, V. A. Alonso, C. Zakaryan, H. Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus |
| description |
Rigid amphipathic fusion inhibitors (RAFIs) are a family of nucleoside derivatives that inhibit the infectivity of several enveloped viruses by interacting with virion envelope lipids and inhibiting fusion between viral and cellular membranes. Here we tested the antiviral activity of two RAFIs, 5-(Perylen-3-ylethynyl)-arabino-uridine (aUY11) and 5-(Perylen-3-ylethynyl)uracil-1-acetic acid (cm1UY11) against African swine fever virus (ASFV), for which no effective vaccine is available. Both compounds displayed a potent, dose-dependent inhibitory effect on ASFV infection in Vero cells. The major antiviral effect was observed when aUY11 and cm1UY11 were added at early stages of infection and maintained during the complete viral cycle. Furthermore, virucidal assay revealed a significant extracellular anti-ASFV activity for both compounds. We also found decrease in the synthesis of early and late viral proteins in Vero cells treated with cm1UY11. Finally, the inhibitory effect of aUY11 and cm1UY11 on ASFV infection in porcine alveolar macrophages was confirmed. Overall, our study has identified novel anti-ASFV compounds with potential for future therapeutic developments. |
| format |
artículo |
| topic_facet |
African swine fever virus Antivirals Antiviral therapy Nucleoside analogues |
| author |
Hakobyan, A. Galindo Barreales, Inmaculada Nañez, A. Arabyan, E. Karalyan, Z. Chistov, A. A. Streshnev, P. P. Korshun, V. A. Alonso, C. Zakaryan, H. |
| author_facet |
Hakobyan, A. Galindo Barreales, Inmaculada Nañez, A. Arabyan, E. Karalyan, Z. Chistov, A. A. Streshnev, P. P. Korshun, V. A. Alonso, C. Zakaryan, H. |
| author_sort |
Hakobyan, A. |
| title |
Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus |
| title_short |
Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus |
| title_full |
Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus |
| title_fullStr |
Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus |
| title_full_unstemmed |
Rigid amphipathic fusion inhibitors demonstrate antiviral activity against African swine fever virus |
| title_sort |
rigid amphipathic fusion inhibitors demonstrate antiviral activity against african swine fever virus |
| publisher |
Microbiology Society |
| publishDate |
2018 |
| url |
http://hdl.handle.net/20.500.12792/564 http://hdl.handle.net/10261/290732 |
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| _version_ |
1767603127144415232 |