Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa)
Background Lupin seed flour has been reported as a causative agent of allergic reactions, especially in patients with allergy to peanut. Previous studies have demonstrated that autoclave treatment can considerably reduce the allergenicity of lupins. Aims The aim of this work was to evaluate the effect of instantaneous controlled pressure drop (détente instantanée contrôlée, DIC) treatment on lupin in vitro allergenicity. Methods Lupin cotyledons were subjected to instantaneous controlled pressure drop at several pressure and time conditions (3, 4.5 and 6 bar for 1, 2 and 3 min, respectively). Immunoreactivity to raw and DIC-treated extracts was evaluated by Western blot using a serum pool from 19 sensitized patients. Results Depending on the operating parameters used during DIC treatment, a reduction in protein solubility of lupin seed was observed. Moreover, drastic modifications in protein profiles were observed after DIC treatment by SDS-PAGE analysis. Western blot experiments showed that the decreases in IgE binding to lupin proteins were associated with the increases in steam pressure and time treatment, and binding was completely abolished by DIC at 6 bar for 3 min. Conclusions The results suggest that DIC treatment could produce a reduction in lupin allergenicity. Copyright © 2007 S. Karger AG.
Main Authors: | , , , , , , , , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
S. Karger AG
2007
|
Subjects: | DIC, Food allergy, In vitro IgE binding, Instantaneous controlled pressure drop, Lupinus albus, Thermal processing, |
Online Access: | http://hdl.handle.net/20.500.12792/2010 http://hdl.handle.net/10261/289891 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-289891 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2898912023-02-17T08:25:09Z Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) Guillamón Fernández, Eva Burbano, C. Cuadrado Hoyos, María Carmen Muzquiz, M. Pedrosa, M. M. Sánchez, M. Cabanillas, B. Crespo, J. F. Rodriguez, J. Haddad, J. Allaf, K. DIC Food allergy In vitro IgE binding Instantaneous controlled pressure drop Lupinus albus Thermal processing Background Lupin seed flour has been reported as a causative agent of allergic reactions, especially in patients with allergy to peanut. Previous studies have demonstrated that autoclave treatment can considerably reduce the allergenicity of lupins. Aims The aim of this work was to evaluate the effect of instantaneous controlled pressure drop (détente instantanée contrôlée, DIC) treatment on lupin in vitro allergenicity. Methods Lupin cotyledons were subjected to instantaneous controlled pressure drop at several pressure and time conditions (3, 4.5 and 6 bar for 1, 2 and 3 min, respectively). Immunoreactivity to raw and DIC-treated extracts was evaluated by Western blot using a serum pool from 19 sensitized patients. Results Depending on the operating parameters used during DIC treatment, a reduction in protein solubility of lupin seed was observed. Moreover, drastic modifications in protein profiles were observed after DIC treatment by SDS-PAGE analysis. Western blot experiments showed that the decreases in IgE binding to lupin proteins were associated with the increases in steam pressure and time treatment, and binding was completely abolished by DIC at 6 bar for 3 min. Conclusions The results suggest that DIC treatment could produce a reduction in lupin allergenicity. Copyright © 2007 S. Karger AG. 2023-02-17T08:25:09Z 2023-02-17T08:25:09Z 2007 artículo International Archives of Allergy and Immunology 145: 9- 14 (2007) 1018-2438 http://hdl.handle.net/20.500.12792/2010 http://hdl.handle.net/10261/289891 10.1159/000107461 1423-0097 en none S. Karger AG |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
DIC Food allergy In vitro IgE binding Instantaneous controlled pressure drop Lupinus albus Thermal processing DIC Food allergy In vitro IgE binding Instantaneous controlled pressure drop Lupinus albus Thermal processing |
spellingShingle |
DIC Food allergy In vitro IgE binding Instantaneous controlled pressure drop Lupinus albus Thermal processing DIC Food allergy In vitro IgE binding Instantaneous controlled pressure drop Lupinus albus Thermal processing Guillamón Fernández, Eva Burbano, C. Cuadrado Hoyos, María Carmen Muzquiz, M. Pedrosa, M. M. Sánchez, M. Cabanillas, B. Crespo, J. F. Rodriguez, J. Haddad, J. Allaf, K. Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) |
description |
Background Lupin seed flour has been reported as a causative agent of allergic reactions, especially in patients with allergy to peanut. Previous studies have demonstrated that autoclave treatment can considerably reduce the allergenicity of lupins. Aims The aim of this work was to evaluate the effect of instantaneous controlled pressure drop (détente instantanée contrôlée, DIC) treatment on lupin in vitro allergenicity. Methods Lupin cotyledons were subjected to instantaneous controlled pressure drop at several pressure and time conditions (3, 4.5 and 6 bar for 1, 2 and 3 min, respectively). Immunoreactivity to raw and DIC-treated extracts was evaluated by Western blot using a serum pool from 19 sensitized patients. Results Depending on the operating parameters used during DIC treatment, a reduction in protein solubility of lupin seed was observed. Moreover, drastic modifications in protein profiles were observed after DIC treatment by SDS-PAGE analysis. Western blot experiments showed that the decreases in IgE binding to lupin proteins were associated with the increases in steam pressure and time treatment, and binding was completely abolished by DIC at 6 bar for 3 min. Conclusions The results suggest that DIC treatment could produce a reduction in lupin allergenicity. Copyright © 2007 S. Karger AG. |
format |
artículo |
topic_facet |
DIC Food allergy In vitro IgE binding Instantaneous controlled pressure drop Lupinus albus Thermal processing |
author |
Guillamón Fernández, Eva Burbano, C. Cuadrado Hoyos, María Carmen Muzquiz, M. Pedrosa, M. M. Sánchez, M. Cabanillas, B. Crespo, J. F. Rodriguez, J. Haddad, J. Allaf, K. |
author_facet |
Guillamón Fernández, Eva Burbano, C. Cuadrado Hoyos, María Carmen Muzquiz, M. Pedrosa, M. M. Sánchez, M. Cabanillas, B. Crespo, J. F. Rodriguez, J. Haddad, J. Allaf, K. |
author_sort |
Guillamón Fernández, Eva |
title |
Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) |
title_short |
Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) |
title_full |
Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) |
title_fullStr |
Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) |
title_full_unstemmed |
Effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (Lupinus albus var Multolupa) |
title_sort |
effect of an instantaneous controlled pressure drop on in vitro allergenicity to lupins (lupinus albus var multolupa) |
publisher |
S. Karger AG |
publishDate |
2007 |
url |
http://hdl.handle.net/20.500.12792/2010 http://hdl.handle.net/10261/289891 |
work_keys_str_mv |
AT guillamonfernandezeva effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT burbanoc effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT cuadradohoyosmariacarmen effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT muzquizm effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT pedrosamm effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT sanchezm effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT cabanillasb effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT crespojf effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT rodriguezj effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT haddadj effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa AT allafk effectofaninstantaneouscontrolledpressuredroponinvitroallergenicitytolupinslupinusalbusvarmultolupa |
_version_ |
1767603019499700224 |