Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie

Marín, Belén; Otero, Alicia; Lugan, Séverine; Espinosa Martín, Juan Carlos; Marín-Moreno, Alba; Vidal, Enric; Hedman, Carlos; Romero, Antonio; Pumarola, Martí; Badiola, Juan J.; Torres, Juan María; Andréoletti, Olivier; Bolea, Rosa; European Commission

Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie

Centro de Investigación en Sanidad Animal (CISA)

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Main Authors:	Marín, Belén, Otero, Alicia, Lugan, Séverine, Espinosa Martín, Juan Carlos, Marín-Moreno, Alba, Vidal, Enric, Hedman, Carlos, Romero, Antonio, Pumarola, Martí, Badiola, Juan J., Torres, Juan María, Andréoletti, Olivier, Bolea, Rosa
Other Authors:	European Commission
Format:	artículo biblioteca
Language:	English
Published:	Nature Publishing Group 2021-08-31
Subjects:	Bovine spongiform encephalopathy, Prion, Disease Susceptibility, PrPSc Proteins,
Online Access:	http://hdl.handle.net/10261/274898 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85113984408
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spelling	dig-inia-es-10261-2748982024-05-18T20:35:58Z Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie Marín, Belén Otero, Alicia Lugan, Séverine Espinosa Martín, Juan Carlos Marín-Moreno, Alba Vidal, Enric Hedman, Carlos Romero, Antonio Pumarola, Martí Badiola, Juan J. Torres, Juan María Andréoletti, Olivier Bolea, Rosa European Commission Ministerio de Ciencia e Innovación (España) Marín, Belén [0000-0002-1590-3347] Otero, Alicia [0000-0001-9075-2764] Espinosa Martín, Juan Carlos [0000-0002-6719-9902] Marín-Moreno, Alba [0000-0002-4023-6398] Vidal, Enric [0000-0002-4965-3286] Hedman, Carlos [0000-0002-0827-110X] Pumarola, Martí[0000-0002-0935-7941] Badiola, Juan J [0000-0002-7173-7216] Torres, Juan María [0000-0003-0443-9232] Andréoletti, Olivier [0000-0002-7369-6016] Bolea, Rosa [0000-0002-2746-3932] Bovine spongiform encephalopathy Prion Disease Susceptibility PrPSc Proteins Centro de Investigación en Sanidad Animal (CISA) Pigs are susceptible to infection with the classical bovine spongiform encephalopathy (C-BSE) agent following experimental inoculation, and PrPSc accumulation was detected in porcine tissues after the inoculation of certain scrapie and chronic wasting disease isolates. However, a robust transmission barrier has been described in this species and, although they were exposed to C-BSE agent in many European countries, no cases of natural transmissible spongiform encephalopathies (TSE) infections have been reported in pigs. Transmission of atypical scrapie to bovinized mice resulted in the emergence of C-BSE prions. Here, we conducted a study to determine if pigs are susceptible to atypical scrapie. To this end, 12, 8-9-month-old minipigs were intracerebrally inoculated with two atypical scrapie sources. Animals were euthanized between 22- and 72-months post inoculation without clinical signs of TSE. All pigs tested negative for PrPSc accumulation by enzyme immunoassay, immunohistochemistry, western blotting and bioassay in porcine PrP mice. Surprisingly, in vitro protein misfolding cyclic amplification demonstrated the presence of C-BSE prions in different brain areas from seven pigs inoculated with both atypical scrapie isolates. Our results suggest that pigs exposed to atypical scrapie prions could become a reservoir for C-BSE and corroborate that C-BSE prions emerge during interspecies passage of atypical scrapie. This work was supported by the project EFA148/16 REDPRION. The project EFA148/16 REDPRION was 65% co-financed by the European Regional Development Fund (ERDF) through the Interreg V-A Spain-France-Andorra program (POCTEFA 2014–2020). POCTEFA aims to reinforce the economic and social integration of the French–Spanish–Andorran border. Its support is focused on developing economic, social and environmental cross-border activities through joint strategies favoring sustainable territorial development. Transmission experiments in transgenic mice were founded by grant PID2019-105837RB-I00 from Spanish Ministerio de Ciencia e Innovación Peer reviewed 2022-07-06T09:32:23Z 2022-07-06T09:32:23Z 2021-08-31 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 11(1): 17428(2022) 2045-2322 http://hdl.handle.net/10261/274898 10.1038/s41598-021-96818-2 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 34465826 2-s2.0-85113984408 https://api.elsevier.com/content/abstract/scopus_id/85113984408 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC//EFA148/16 REDPRION info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105837RB-I00/ES/EVOLUCION A TRAVES DE LA BARRERA DE ESPECIE DEL POTECIAL ZOONOTICO DE LOS PRIONES EMERGENTES/ Scientific reports Publisher's version https://doi.org/10.1038/s41598-021-96818-2 Sí open Nature Publishing Group
institution	INIA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-inia-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del INIA España
language	English
topic	Bovine spongiform encephalopathy Prion Disease Susceptibility PrPSc Proteins Bovine spongiform encephalopathy Prion Disease Susceptibility PrPSc Proteins
spellingShingle	Bovine spongiform encephalopathy Prion Disease Susceptibility PrPSc Proteins Bovine spongiform encephalopathy Prion Disease Susceptibility PrPSc Proteins Marín, Belén Otero, Alicia Lugan, Séverine Espinosa Martín, Juan Carlos Marín-Moreno, Alba Vidal, Enric Hedman, Carlos Romero, Antonio Pumarola, Martí Badiola, Juan J. Torres, Juan María Andréoletti, Olivier Bolea, Rosa Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
description	Centro de Investigación en Sanidad Animal (CISA)
author2	European Commission
author_facet	European Commission Marín, Belén Otero, Alicia Lugan, Séverine Espinosa Martín, Juan Carlos Marín-Moreno, Alba Vidal, Enric Hedman, Carlos Romero, Antonio Pumarola, Martí Badiola, Juan J. Torres, Juan María Andréoletti, Olivier Bolea, Rosa
format	artículo
topic_facet	Bovine spongiform encephalopathy Prion Disease Susceptibility PrPSc Proteins
author	Marín, Belén Otero, Alicia Lugan, Séverine Espinosa Martín, Juan Carlos Marín-Moreno, Alba Vidal, Enric Hedman, Carlos Romero, Antonio Pumarola, Martí Badiola, Juan J. Torres, Juan María Andréoletti, Olivier Bolea, Rosa
author_sort	Marín, Belén
title	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_short	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_full	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_fullStr	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_full_unstemmed	Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie
title_sort	classical bse prions emerge from asymptomatic pigs challenged with atypical/nor98 scrapie
publisher	Nature Publishing Group
publishDate	2021-08-31
url	http://hdl.handle.net/10261/274898 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85113984408
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Classical BSE prions emerge from asymptomatic pigs challenged with atypical/Nor98 scrapie

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