Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss)
14 Pág.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2022-05
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| Subjects: | Adjuvants, Bacillus subtilis, B cells, Inflammation, Oral vaccines, Probiotics, Rainbow trout, |
| Online Access: | http://hdl.handle.net/10261/271643 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85127532451 |
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Adjuvants Bacillus subtilis B cells Inflammation Oral vaccines Probiotics Rainbow trout Adjuvants Bacillus subtilis B cells Inflammation Oral vaccines Probiotics Rainbow trout |
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Adjuvants Bacillus subtilis B cells Inflammation Oral vaccines Probiotics Rainbow trout Adjuvants Bacillus subtilis B cells Inflammation Oral vaccines Probiotics Rainbow trout Docando, Félix Nuñez-Ortiz, Noelia Serra, Claudia R. Arense, Paula Enes, Paula Oliva-Teles, Aires Díaz-Rosales, Patricia Tafalla, Carolina Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) |
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14 Pág. |
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Ministerio de Ciencia, Innovación y Universidades (España) |
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Ministerio de Ciencia, Innovación y Universidades (España) Docando, Félix Nuñez-Ortiz, Noelia Serra, Claudia R. Arense, Paula Enes, Paula Oliva-Teles, Aires Díaz-Rosales, Patricia Tafalla, Carolina |
| format |
artículo |
| topic_facet |
Adjuvants Bacillus subtilis B cells Inflammation Oral vaccines Probiotics Rainbow trout |
| author |
Docando, Félix Nuñez-Ortiz, Noelia Serra, Claudia R. Arense, Paula Enes, Paula Oliva-Teles, Aires Díaz-Rosales, Patricia Tafalla, Carolina |
| author_sort |
Docando, Félix |
| title |
Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) |
| title_short |
Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) |
| title_full |
Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) |
| title_fullStr |
Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) |
| title_full_unstemmed |
Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) |
| title_sort |
mucosal and systemic immune effects of bacillus subtilis in rainbow trout (oncorhynchus mykiss) |
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Elsevier |
| publishDate |
2022-05 |
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http://hdl.handle.net/10261/271643 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85127532451 |
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1802819626386587648 |
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dig-inia-es-10261-2716432024-05-16T20:37:10Z Mucosal and systemic immune effects of Bacillus subtilis in rainbow trout (Oncorhynchus mykiss) Docando, Félix Nuñez-Ortiz, Noelia Serra, Claudia R. Arense, Paula Enes, Paula Oliva-Teles, Aires Díaz-Rosales, Patricia Tafalla, Carolina Ministerio de Ciencia, Innovación y Universidades (España) Comunidad de Madrid European Commission Foundation for Science and Technology Docando, F [0000-0001-8835-9349] Nuñez-Ortiz, N [0000-0003-2524-0759] Enes, Paula [0000-0002-6248-0500] Oliva-Teles, A [0000-0002-5730-836X] Díaz-Rosales, Patricia [0000-0001-6965-3702] Tafalla, Carolina [0000-0002-0860-2976] Adjuvants Bacillus subtilis B cells Inflammation Oral vaccines Probiotics Rainbow trout 14 Pág. Bacillus spp. are well known for their probiotic properties. Hence, the long-term feeding of Bacillus spp. strains to different fish species has been proved to confer beneficial effects regarding growth or pathogen resistance, among others. However, whether these strains could function as mucosal adjuvants, up-regulating immune responses after a single administration, has not yet been investigated in fish. Thus, in the current work, we have performed a series of experiments in rainbow trout (Oncorhynchus mykiss) aimed at establishing the potential of two Bacillus subtilis spore-forming strains, designated as ABP1 and ABP2, as oral adjuvants/immunostimulants. As an initial step, we evaluated their transcriptional effects on the rainbow trout intestinal epithelial cell line RTgutGC, and in gut tissue explants incubated ex vivo with the two strains. Their capacity to adhere to RTgutGC cells was also evaluated by flow cytometry. Although both strains had the capacity to modulate the transcription of several genes related to innate and adaptive immune responses, it was the ABP1 strain that led to stronger transcriptional effects, also exerting a higher binding capacity to intestinal epithelial cells. Consequently, we selected this strain to establish its effects on splenic B cells upon in vitro exposure as well as to determine the transcriptional effects exerted in the spleen, kidney, and gut after a single oral administration of the bacteria. Our results showed that B. subtilis ABP1 had the capacity to modulate the proliferation, IgM secreting capacity and MHC II surface expression of splenic B cells. Finally, we confirmed that this strain also induced the transcription of genes involved in inflammation, antimicrobial genes, and genes involved in T cell responses upon a single oral administration. Our results provide valuable information regarding how B. subtilis modulates the immune response of rainbow trout, pointing to the usefulness of the ABP1 strain to design novel oral vaccination strategies for aquaculture. This work was financed by the Spanish Ministry of Science, Innovation and Universities (projects AGL2017-85494-C2-1-R and PID2020-113268RB-I00), by the Comunidad de Madrid (grant 2016-T1/BIO-1672 and 2018-T2/BIO-10874) and the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM). P.E and C.S. had a scientific employment contract supported by national funds through FCT - Foundation for Science and Technology. Peer reviewed 2022-06-06T08:37:14Z 2022-06-06T08:37:14Z 2022-05 artículo http://purl.org/coar/resource_type/c_6501 Fish and Shellfish Immunology 124; 142-155 (2022) 1050-4648 http://hdl.handle.net/10261/271643 10.1016/j.fsi.2022.03.040 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 35367376 2-s2.0-85127532451 https://api.elsevier.com/content/abstract/scopus_id/85127532451 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85494-C2-1-R/ES/BUSQUEDA DE ADYUVANTES EFECTIVOS PARA VACUNACION ORAL DE PECES/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113268RB-I00/ES/RELACION ENTRE LA MICROBIOTA, COMPOSICION DE LA DIETA Y RESPUESTA A VACUNACION EN TRUCHA ARCOIRIS (ONCORHYNCHUS MYKISS)/ S2016/T1/BIO-1672 S2018/T2/BIO-10874 info:eu-repo/grantAgreement/EC/2016 725061 TEMUBLYM Fish & shellfish immunology Publisher's version https://doi.org/10.1016/j.fsi.2022.03.040 Sí open Elsevier |