Plant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
An in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibberellic acid (GA3). A máximum of 10.6 shoots was obtained with 16.1 μM NAA, 17.1 μM IAA, 6.3 x 10-9 μM GA3 and 0.0023 μM BA. A máximum of 4.0 roots on each shoot with 5.4 μM NAA, 17.1 μM IAA, 0.001 μM AG3 and 4.6 x 10-9 μM BA. Máximum shoot and root length was obtained with a minimum of GA3, but a máximum IAA. GA3, was the main factor controlling shoot and root induction and elongation.
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| Main Authors: | , , , , |
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| Format: | Artículo de revista biblioteca |
| Language: | English |
| Published: |
Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
2020-06-09T15:24:17Z
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| Subjects: | 6-benzyladenine, indole-3-acetic acid, a-naftalenacetic acid, gibberellic acid, shoot induction, rooting, |
| Online Access: | https://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003 https://bibliotecadigital.infor.cl/handle/20.500.12220/29586 |
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