Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L)
Antibiotics can be uptaken by plants from soil desorption or directly from irrigation water, but their metabolization pathways in plants are largely unknown. In this paper, an analytical workflow based on high-resolution mass spectrometry was applied for the systematic identification of biotransformation products of ofloxacin in lettuce. The targeted metabolites were selected by comparing the mass chromatograms of exposed with control samples using an advanced spectra-processing method (Fragment Ion Search). The innovative methodology presented allowed us to identify a total of 11 metabolites, including 5 ofloxacin metabolites that are being reported for the first time in plants. Accordingly, major transformation pathways were proposed revealing insight into how ofloxacin and related chemicals are metabolized in lettuce. Furthermore, the influence of biotransformation on potential residual antimicrobial activity of identified compounds was discussed. Human exposure to antibiotics at doses below the minimum inhibitory concentrations is crucial in human risk assessment, including food ingestion; however, in the case of ofloxacin presented results reveal that plant metabolites should also be considered so as not to underestimate their risk.
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Elsevier
2020-01-21
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Subjects: | Antibiotics, Plant metabolites, High-resolution mass spectrometry, Biotransformation pathways, |
Online Access: | http://hdl.handle.net/10261/198565 http://dx.doi.org/10.13039/501100000780 |
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dig-idaea-es-10261-1985652020-05-29T10:54:43Z Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) Tadić, Đorđe Gramblicka, Michal Mistrik, Robert Flores, Cintia Piña, Benjamín Bayona Termens, Josep María European Commission Flores, Cintia [0000-0002-7766-5639] Piña, Benjamín [0000-0001-9216-276] Bayona Termens, Josep María [0000-0001-5042-837X] Antibiotics Plant metabolites High-resolution mass spectrometry Biotransformation pathways Antibiotics can be uptaken by plants from soil desorption or directly from irrigation water, but their metabolization pathways in plants are largely unknown. In this paper, an analytical workflow based on high-resolution mass spectrometry was applied for the systematic identification of biotransformation products of ofloxacin in lettuce. The targeted metabolites were selected by comparing the mass chromatograms of exposed with control samples using an advanced spectra-processing method (Fragment Ion Search). The innovative methodology presented allowed us to identify a total of 11 metabolites, including 5 ofloxacin metabolites that are being reported for the first time in plants. Accordingly, major transformation pathways were proposed revealing insight into how ofloxacin and related chemicals are metabolized in lettuce. Furthermore, the influence of biotransformation on potential residual antimicrobial activity of identified compounds was discussed. Human exposure to antibiotics at doses below the minimum inhibitory concentrations is crucial in human risk assessment, including food ingestion; however, in the case of ofloxacin presented results reveal that plant metabolites should also be considered so as not to underestimate their risk. The work presented in this paper is part of a project that has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 675530. Special thanks to Dr. Nikiforos Alygizakis and Dr. Josep Caixach, for their advice on the elucidation of metabolites. Peer reviewed 2020-01-22T09:26:04Z 2020-01-22T09:26:04Z 2020-01-21 artículo http://purl.org/coar/resource_type/c_6501 Environmental Pollution 114002 (2020) http://hdl.handle.net/10261/198565 10.1016/j.envpol.2020.114002 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/675530 Postprint https://doi.org/10.1016/j.envpol.2020.114002 Sí open Elsevier |
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Antibiotics Plant metabolites High-resolution mass spectrometry Biotransformation pathways Antibiotics Plant metabolites High-resolution mass spectrometry Biotransformation pathways |
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Antibiotics Plant metabolites High-resolution mass spectrometry Biotransformation pathways Antibiotics Plant metabolites High-resolution mass spectrometry Biotransformation pathways Tadić, Đorđe Gramblicka, Michal Mistrik, Robert Flores, Cintia Piña, Benjamín Bayona Termens, Josep María Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) |
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Antibiotics can be uptaken by plants from soil desorption or directly from irrigation water, but their metabolization pathways in plants are largely unknown. In this paper, an analytical workflow based on high-resolution mass spectrometry was applied for the systematic identification of biotransformation products of ofloxacin in lettuce. The targeted metabolites were selected by comparing the mass chromatograms of exposed with control samples using an advanced spectra-processing method (Fragment Ion Search). The innovative methodology presented allowed us to identify a total of 11 metabolites, including 5 ofloxacin metabolites that are being reported for the first time in plants. Accordingly, major transformation pathways were proposed revealing insight into how ofloxacin and related chemicals are metabolized in lettuce. Furthermore, the influence of biotransformation on potential residual antimicrobial activity of identified compounds was discussed. Human exposure to antibiotics at doses below the minimum inhibitory concentrations is crucial in human risk assessment, including food ingestion; however, in the case of ofloxacin presented results reveal that plant metabolites should also be considered so as not to underestimate their risk. |
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European Commission |
author_facet |
European Commission Tadić, Đorđe Gramblicka, Michal Mistrik, Robert Flores, Cintia Piña, Benjamín Bayona Termens, Josep María |
format |
artículo |
topic_facet |
Antibiotics Plant metabolites High-resolution mass spectrometry Biotransformation pathways |
author |
Tadić, Đorđe Gramblicka, Michal Mistrik, Robert Flores, Cintia Piña, Benjamín Bayona Termens, Josep María |
author_sort |
Tadić, Đorđe |
title |
Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) |
title_short |
Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) |
title_full |
Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) |
title_fullStr |
Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) |
title_full_unstemmed |
Elucidating biotransformation pathways of ofloxacin in lettuce (Lactuca sativa L) |
title_sort |
elucidating biotransformation pathways of ofloxacin in lettuce (lactuca sativa l) |
publisher |
Elsevier |
publishDate |
2020-01-21 |
url |
http://hdl.handle.net/10261/198565 http://dx.doi.org/10.13039/501100000780 |
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