Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability
9 pages, 6 figures.-- PMID: 15495411 [PubMed].-- Printed version published Sep 2001.
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| Language: | English |
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John Wiley & Sons
2004-08-17
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| Subjects: | Immunoaffinity chromatography, PCBs, PCDD/Fs, Halogenated aromatic compounds, Structure-property relationship, |
| Online Access: | http://hdl.handle.net/10261/11460 |
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dig-idaea-es-10261-114602019-02-12T10:04:10Z Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability Concejero, Miguel A. Abad Holgado, Esteban Rivera Aranda, Josep Herradón García, Bernardo González, María J. Frutos, Mercedes de Immunoaffinity chromatography PCBs PCDD/Fs Halogenated aromatic compounds Structure-property relationship 9 pages, 6 figures.-- PMID: 15495411 [PubMed].-- Printed version published Sep 2001. Part of this work is included in patents (ES2167238 and WO0190755). The retention of organochlorinated compounds on an immunochromatographic column is studied. The compounds considered are usually found together in real samples of environmental concern, and include chlorinated biphenyls, chlorinated dibenzo-p-dioxins, chlorinated dibenzofurans, and organochlorinated insecticides. The different retention observed for different compounds is interpreted in light of the structural similarities of the compound studied with that used as a hapten to raise the antibodies employed as ligands in the immunochromatographic column. Differences in retention of the organochlorinated compounds on the immunocolumn make it possible to fractionate them. Mixtures of phosphate buffer solution and ACN in different ratios were used as desorption agents. Depending on the percentage of ACN employed, different fractionations of the organochlorinated compounds are obtained. The use of 20% ACN allows fractionation of most insecticides from chlorinated biphenyls, dioxins, and furans. Besides, chlorinated biphenyls of different chlorination degree can be separated. Moreover, pentachlorinated dioxins and pentachlorinated furans are separated from hexachlorinated biphenyls. Fractionation is of critical importance from a practical point of view, as it avoids some of the interferences that otherwise take place during the subsequent gas chromatography/mass spectrometry (GC/MS) analysis of these compounds. In this way, immunochromatography makes it possible to perform in a single step all the processes (extraction, clean-up, concentration, and fractionation) that are needed prior to the GC/MS analysis of these analytes in aqueous samples. An additional advantage of this method is the reusability of the immunoaffinity chromatography column for more than 500 times. This work has been supported by the Spanish Ministry of Science and Technology (Projects INIA CAL00-041 and BQU2001-2270) and by the Spanish Ministry of Education and Culture (Project 2FD97-0621). Peer reviewed 2009-03-11T08:44:59Z 2009-03-11T08:44:59Z 2004-08-17 artículo http://purl.org/coar/resource_type/c_6501 Journal of Separation Science 27(13): 1093-1101 (2004) 1615-9306 http://hdl.handle.net/10261/11460 10.1002/jssc.200301691 1615-9314 en http://dx.doi.org/10.1002/jssc.200301691 none 88 bytes text/plain John Wiley & Sons |
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Immunoaffinity chromatography PCBs PCDD/Fs Halogenated aromatic compounds Structure-property relationship Immunoaffinity chromatography PCBs PCDD/Fs Halogenated aromatic compounds Structure-property relationship |
| spellingShingle |
Immunoaffinity chromatography PCBs PCDD/Fs Halogenated aromatic compounds Structure-property relationship Immunoaffinity chromatography PCBs PCDD/Fs Halogenated aromatic compounds Structure-property relationship Concejero, Miguel A. Abad Holgado, Esteban Rivera Aranda, Josep Herradón García, Bernardo González, María J. Frutos, Mercedes de Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability |
| description |
9 pages, 6 figures.-- PMID: 15495411 [PubMed].-- Printed version published Sep 2001. |
| format |
artículo |
| topic_facet |
Immunoaffinity chromatography PCBs PCDD/Fs Halogenated aromatic compounds Structure-property relationship |
| author |
Concejero, Miguel A. Abad Holgado, Esteban Rivera Aranda, Josep Herradón García, Bernardo González, María J. Frutos, Mercedes de |
| author_facet |
Concejero, Miguel A. Abad Holgado, Esteban Rivera Aranda, Josep Herradón García, Bernardo González, María J. Frutos, Mercedes de |
| author_sort |
Concejero, Miguel A. |
| title |
Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability |
| title_short |
Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability |
| title_full |
Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability |
| title_fullStr |
Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability |
| title_full_unstemmed |
Differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. Interpretation and applicability |
| title_sort |
differences in retention of dioxin-like compounds and organochlorinated insecticides on an immunochromatographic column. interpretation and applicability |
| publisher |
John Wiley & Sons |
| publishDate |
2004-08-17 |
| url |
http://hdl.handle.net/10261/11460 |
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