Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry
10 páginas, 5 tablas, 3 figuras
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2014
|
| Subjects: | High resolution mass spectrometry, Lipophilic marine toxins, Identification and confirmation, Quantification, Validation method, Uncertainty, |
| Online Access: | http://hdl.handle.net/10261/103041 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-idaea-es-10261-103041 |
|---|---|
| record_format |
koha |
| spelling |
dig-idaea-es-10261-1030412017-06-07T10:14:19Z Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry Domènech, Albert Cortés, Nuria Palacios, Óscar Franco, José M. Riobó, Pilar Llerena, José J. Vichi, Stefania Caixach Gamisans, Josep High resolution mass spectrometry Lipophilic marine toxins Identification and confirmation Quantification Validation method Uncertainty 10 páginas, 5 tablas, 3 figuras A multitoxin method has been developed for quantification and confirmation of lipophilic marine biotoxins in mussels by liquid chromatography coupled to high resolution mass spectrometry (HRMS), using an Orbitrap-Exactive HCD mass spectrometer. Okadaic acid (OA), yessotoxin, azaspiracid-1, gymnodimine, 13-desmethyl spirolide C, pectenotoxin-2 and Brevetoxin B were analyzed as representative compounds of each lipophilic toxin group. HRMS identification and confirmation criteria were established. Fragment and isotope ions and ion ratios were studied and evaluated for confirmation purpose. In depth characterization of full scan and fragmentation spectrum of the main toxins were carried out. Accuracy (trueness and precision), linearity, calibration curve check, limit of quantification (LOQ) and specificity were the parameters established for the method validation. The validation was performed at 0.5 times the current European Union permitted levels. The method performed very well for the parameters investigated. The trueness, expressed as recovery, ranged from 80% to 94%, the precision, expressed as intralaboratory reproducibility, ranged from 5% to 22% and the LOQs range from 0.9 to 4.8 pg on column. Uncertainty of the method was also estimated for OA, using a certified reference material. A top-down approach considering two main contributions: those arising from the trueness studies and those coming from the precision's determination, was used. An overall expanded uncertainty of 38% was obtained Peer reviewed 2014-10-08T11:08:20Z 2014-10-08T11:08:20Z 2014 artículo http://purl.org/coar/resource_type/c_6501 Journal of Cromatography A 1328: 16-25 (2014) 0021-9673 http://hdl.handle.net/10261/103041 10.1016/j.chroma.2013.12.071 1873-3778 en Postprint http://dx.doi.org/10.1016/j.chroma.2013.12.071 Sí open Elsevier |
| institution |
IDAEA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-idaea-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IDAEA España |
| language |
English |
| topic |
High resolution mass spectrometry Lipophilic marine toxins Identification and confirmation Quantification Validation method Uncertainty High resolution mass spectrometry Lipophilic marine toxins Identification and confirmation Quantification Validation method Uncertainty |
| spellingShingle |
High resolution mass spectrometry Lipophilic marine toxins Identification and confirmation Quantification Validation method Uncertainty High resolution mass spectrometry Lipophilic marine toxins Identification and confirmation Quantification Validation method Uncertainty Domènech, Albert Cortés, Nuria Palacios, Óscar Franco, José M. Riobó, Pilar Llerena, José J. Vichi, Stefania Caixach Gamisans, Josep Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry |
| description |
10 páginas, 5 tablas, 3 figuras |
| format |
artículo |
| topic_facet |
High resolution mass spectrometry Lipophilic marine toxins Identification and confirmation Quantification Validation method Uncertainty |
| author |
Domènech, Albert Cortés, Nuria Palacios, Óscar Franco, José M. Riobó, Pilar Llerena, José J. Vichi, Stefania Caixach Gamisans, Josep |
| author_facet |
Domènech, Albert Cortés, Nuria Palacios, Óscar Franco, José M. Riobó, Pilar Llerena, José J. Vichi, Stefania Caixach Gamisans, Josep |
| author_sort |
Domènech, Albert |
| title |
Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry |
| title_short |
Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry |
| title_full |
Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry |
| title_fullStr |
Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry |
| title_full_unstemmed |
Determination of lipophilic marine toxins in mussels. Quantification and confirmation criteria using high resolution mass spectrometry |
| title_sort |
determination of lipophilic marine toxins in mussels. quantification and confirmation criteria using high resolution mass spectrometry |
| publisher |
Elsevier |
| publishDate |
2014 |
| url |
http://hdl.handle.net/10261/103041 |
| work_keys_str_mv |
AT domenechalbert determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT cortesnuria determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT palaciososcar determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT francojosem determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT riobopilar determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT llerenajosej determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT vichistefania determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry AT caixachgamisansjosep determinationoflipophilicmarinetoxinsinmusselsquantificationandconfirmationcriteriausinghighresolutionmassspectrometry |
| _version_ |
1777669259539775488 |