A new methodology to obtain wine yeast strains overproducing mannoproteins

A new methodology to obtain wine yeast strains overproducing mannoproteins

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I Congreso Nacional de Biotecnología Enológica. Invinotec. Vinomio. León. 26-31 Octubre 2010. -- Premio Accésit del Concurso de pósters.

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Main Authors: Quirós Asensio, Manuel, González Ramos, Daniel, Tabera, Laura, Morales, Pilar, Pérez-Través, Laura, Barcenilla, José M.
Format: póster de congreso biblioteca
Language:English
Published: 2010
Online Access:http://hdl.handle.net/10261/48094
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spelling dig-icvv-es-10261-480942018-01-16T10:44:56Z A new methodology to obtain wine yeast strains overproducing mannoproteins Quirós Asensio, Manuel González Ramos, Daniel Tabera, Laura Morales, Pilar Pérez-Través, Laura Barcenilla, José M. I Congreso Nacional de Biotecnología Enológica. Invinotec. Vinomio. León. 26-31 Octubre 2010. -- Premio Accésit del Concurso de pósters. Yeast mannoproteins are highly glycosylated proteins that are covalently bound to the β-1,3-glucan present in the yeast cell wall. These complex macromolecules are structurally composed of polymers of sugars, mainly mannose, that are covalently linked to peptides and represent around 40% of the cell wall dry weight. Yeast mannoproteins contribute to several aspects of wine quality by protecting against protein haze, reducing astringency, retaining aroma and other undesired compounds such as OTA and stimulating the growth of lactic-acid bacteria. Therefore, the development of a non-recombinant method to obtain enological yeast strains overproducing mannoproteins would be very useful. Previous works developed in our group have allowed the identification of several genetic determinants for the release of wine yeast mannoproteins (Gonzalez-Ramos and Gonzalez, 2006; Gonzalez-Ramos et al., 2008). Among the different recombinant genotypes constructed and studied, strains deleted for KNR4 (from Killer Nine Resistant, also known as SMI1), a gene involved in β-1,3-glucan biosynthesis yielded optimal results (Gonzalez-Ramos et al., 2008). In the present paper, a new methodology for the selection of non-recombinant mannoprotein overproducing yeast strains based on their resistance to the killer 9 toxin of Williopsis saturnus is developed. Peer reviewed 2012-04-11T09:10:24Z 2012-04-11T09:10:24Z 2010 póster de congreso http://purl.org/coar/resource_type/c_6670 http://hdl.handle.net/10261/48094 en open
institution ICVV ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
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libraryname Biblioteca del ICVV España
language English
description I Congreso Nacional de Biotecnología Enológica. Invinotec. Vinomio. León. 26-31 Octubre 2010. -- Premio Accésit del Concurso de pósters.
format póster de congreso
author Quirós Asensio, Manuel
González Ramos, Daniel
Tabera, Laura
Morales, Pilar
Pérez-Través, Laura
Barcenilla, José M.
spellingShingle Quirós Asensio, Manuel
González Ramos, Daniel
Tabera, Laura
Morales, Pilar
Pérez-Través, Laura
Barcenilla, José M.
A new methodology to obtain wine yeast strains overproducing mannoproteins
author_facet Quirós Asensio, Manuel
González Ramos, Daniel
Tabera, Laura
Morales, Pilar
Pérez-Través, Laura
Barcenilla, José M.
author_sort Quirós Asensio, Manuel
title A new methodology to obtain wine yeast strains overproducing mannoproteins
title_short A new methodology to obtain wine yeast strains overproducing mannoproteins
title_full A new methodology to obtain wine yeast strains overproducing mannoproteins
title_fullStr A new methodology to obtain wine yeast strains overproducing mannoproteins
title_full_unstemmed A new methodology to obtain wine yeast strains overproducing mannoproteins
title_sort new methodology to obtain wine yeast strains overproducing mannoproteins
publishDate 2010
url http://hdl.handle.net/10261/48094
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