Tetranychus urticae mites do not mount an induced immune response against bacteria
The genome of the spider mite Tetranychus urticae, a herbivore, is missing important elements of the canonical Drosophila immune pathways necessary to fight bacterial infections. However, it is not known whether spider mites can mount an immune response and survive bacterial infection. In other chelicerates, bacterial infection elicits a response mediated by immune effectors leading to the survival of infected organisms. In T. urticae, infection by either Escherichia coli or Bacillus megaterium did not elicit a response as assessed through genome-wide transcriptomic analysis. In line with this, spider mites died within days even upon injection with low doses of bacteria that are non-pathogenic to Drosophila. Moreover, bacterial populations grew exponentially inside the infected spider mites. By contrast, Sancassania berlesei, a litter-dwelling mite, controlled bacterial proliferation and resisted infections with both Gram-negative and Gram-positive bacteria lethal to T. urticae. This differential mortality between mite species was absent when mites were infected with heat-killed bacteria. Also, we found that spider mites harbour in their gut 1000-fold less bacteria than S. berlesei. We show that T. urticae has lost the capacity to mount an induced immune response against bacteria, in contrast to other mites and chelicerates but similarly to the phloem feeding aphid Acyrthosiphon pisum. Hence, our results reinforce the putative evolutionary link between ecological conditions regarding exposure to bacteria and the architecture of the immune response.
Main Authors: | , , , , , , , , , , |
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Format: | artículo biblioteca |
Language: | English |
Published: |
Royal Society (Great Britain)
2017-06-14
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Subjects: | Host-parasite interactions, Tetranychus urticae, Sancassania berlesei,, Microbiota, Immunity, |
Online Access: | http://hdl.handle.net/10261/193608 http://dx.doi.org/10.13039/501100000023 http://dx.doi.org/10.13039/501100000092 http://dx.doi.org/10.13039/100008762 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003130 http://dx.doi.org/10.13039/501100005635 http://dx.doi.org/10.13039/501100001871 |
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dig-icvv-es-10261-1936082021-12-28T16:07:58Z Tetranychus urticae mites do not mount an induced immune response against bacteria Santos-Matos, G. Wybouw, Nicky Martins, Nelson E. Zelé, Flore Riga, Maria Leitão, A.B. Vontas, John Grbić, Miodrag Van Leeuwen, Thomas Magalhães, Sara Sucena, Élio European Commission Research Foundation - Flanders Fundação Calouste Gulbenkian Fundação para a Ciência e a Tecnologia (Portugal) Government of Canada Genome Canada Ontario Genomics Institute Ontario Research Fund Martins, Nelson E. [0000-0002-3923-2998] Zelé, Flore [0000-0003-2954-5488] Vontas, John [0000-0002-8704-2574] Sucena, Élio [0000-0001-8810-870X] Host-parasite interactions Tetranychus urticae Sancassania berlesei, Microbiota Immunity The genome of the spider mite Tetranychus urticae, a herbivore, is missing important elements of the canonical Drosophila immune pathways necessary to fight bacterial infections. However, it is not known whether spider mites can mount an immune response and survive bacterial infection. In other chelicerates, bacterial infection elicits a response mediated by immune effectors leading to the survival of infected organisms. In T. urticae, infection by either Escherichia coli or Bacillus megaterium did not elicit a response as assessed through genome-wide transcriptomic analysis. In line with this, spider mites died within days even upon injection with low doses of bacteria that are non-pathogenic to Drosophila. Moreover, bacterial populations grew exponentially inside the infected spider mites. By contrast, Sancassania berlesei, a litter-dwelling mite, controlled bacterial proliferation and resisted infections with both Gram-negative and Gram-positive bacteria lethal to T. urticae. This differential mortality between mite species was absent when mites were infected with heat-killed bacteria. Also, we found that spider mites harbour in their gut 1000-fold less bacteria than S. berlesei. We show that T. urticae has lost the capacity to mount an induced immune response against bacteria, in contrast to other mites and chelicerates but similarly to the phloem feeding aphid Acyrthosiphon pisum. Hence, our results reinforce the putative evolutionary link between ecological conditions regarding exposure to bacteria and the architecture of the immune response. N.W. was supported by a Marie Sklodowska-Curie Action (MSCA) Individual fellowship (658795-DOGMITE) of Horizon 2020. T.V.L. acknowledges funding from the Fund for Scientific Research Flanders (FWO) (grant nos. G009312N and G053815N), the European Commission (EC contract 618105) via FACCE ERA-NET Plus and FACCE-JP (Genomite, project ID 137 via NWO). This work was supported by Instituto Gulbenkian de Ciencia/Fundacao Calouste Gulbenkian to E.S. and by Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) grant nos. ANR/BIA-EVF/0013/2012 to S.M. and Isabelle Olivieri and FCT-TUBITAK/0001/2014 to S.M. and Ibrahim Cakmak. M.G. acknowledges funding from the Government ofCanada through Genome Canada and the Ontario Genomics Institute (OGI-046), Ontario Research Fund – Global Leadership in Genomics and Life Sciences GL2-01-035, NSERC Strategic Grant STPGP322206-05 and JGI Community Sequencing Program grant no. 777506. Peer reviewed 2019-10-28T13:22:26Z 2019-10-28T13:22:26Z 2017-06-14 artículo http://purl.org/coar/resource_type/c_6501 Proceedings of the Royal Society B: Biological Sciences 284(1856): 20170401 (2017) 0962-8452 http://hdl.handle.net/10261/193608 10.1098/rspb.2017.0401 1471-2954 http://dx.doi.org/10.13039/501100000023 http://dx.doi.org/10.13039/501100000092 http://dx.doi.org/10.13039/100008762 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003130 http://dx.doi.org/10.13039/501100005635 http://dx.doi.org/10.13039/501100001871 28592670 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/658795 info:eu-repo/grantAgreement/EC/FP7/618105 http://dx.doi.org/10.1098/rspb.2017.0401 Sí none Royal Society (Great Britain) |
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Host-parasite interactions Tetranychus urticae Sancassania berlesei, Microbiota Immunity Host-parasite interactions Tetranychus urticae Sancassania berlesei, Microbiota Immunity |
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Host-parasite interactions Tetranychus urticae Sancassania berlesei, Microbiota Immunity Host-parasite interactions Tetranychus urticae Sancassania berlesei, Microbiota Immunity Santos-Matos, G. Wybouw, Nicky Martins, Nelson E. Zelé, Flore Riga, Maria Leitão, A.B. Vontas, John Grbić, Miodrag Van Leeuwen, Thomas Magalhães, Sara Sucena, Élio Tetranychus urticae mites do not mount an induced immune response against bacteria |
description |
The genome of the spider mite Tetranychus urticae, a herbivore, is missing
important elements of the canonical Drosophila immune pathways necessary
to fight bacterial infections. However, it is not known whether spider mites
can mount an immune response and survive bacterial infection. In other chelicerates, bacterial infection elicits a response mediated by immune effectors
leading to the survival of infected organisms. In T. urticae, infection by either
Escherichia coli or Bacillus megaterium did not elicit a response as assessed
through genome-wide transcriptomic analysis. In line with this, spider
mites died within days even upon injection with low doses of bacteria
that are non-pathogenic to Drosophila. Moreover, bacterial populations
grew exponentially inside the infected spider mites. By contrast, Sancassania
berlesei, a litter-dwelling mite, controlled bacterial proliferation and resisted
infections with both Gram-negative and Gram-positive bacteria lethal to
T. urticae. This differential mortality between mite species was absent when
mites were infected with heat-killed bacteria. Also, we found that spider
mites harbour in their gut 1000-fold less bacteria than S. berlesei. We show
that T. urticae has lost the capacity to mount an induced immune response
against bacteria, in contrast to other mites and chelicerates but similarly to
the phloem feeding aphid Acyrthosiphon pisum. Hence, our results reinforce
the putative evolutionary link between ecological conditions regarding
exposure to bacteria and the architecture of the immune response. |
author2 |
European Commission |
author_facet |
European Commission Santos-Matos, G. Wybouw, Nicky Martins, Nelson E. Zelé, Flore Riga, Maria Leitão, A.B. Vontas, John Grbić, Miodrag Van Leeuwen, Thomas Magalhães, Sara Sucena, Élio |
format |
artículo |
topic_facet |
Host-parasite interactions Tetranychus urticae Sancassania berlesei, Microbiota Immunity |
author |
Santos-Matos, G. Wybouw, Nicky Martins, Nelson E. Zelé, Flore Riga, Maria Leitão, A.B. Vontas, John Grbić, Miodrag Van Leeuwen, Thomas Magalhães, Sara Sucena, Élio |
author_sort |
Santos-Matos, G. |
title |
Tetranychus urticae mites do not mount an induced immune response against bacteria |
title_short |
Tetranychus urticae mites do not mount an induced immune response against bacteria |
title_full |
Tetranychus urticae mites do not mount an induced immune response against bacteria |
title_fullStr |
Tetranychus urticae mites do not mount an induced immune response against bacteria |
title_full_unstemmed |
Tetranychus urticae mites do not mount an induced immune response against bacteria |
title_sort |
tetranychus urticae mites do not mount an induced immune response against bacteria |
publisher |
Royal Society (Great Britain) |
publishDate |
2017-06-14 |
url |
http://hdl.handle.net/10261/193608 http://dx.doi.org/10.13039/501100000023 http://dx.doi.org/10.13039/501100000092 http://dx.doi.org/10.13039/100008762 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003130 http://dx.doi.org/10.13039/501100005635 http://dx.doi.org/10.13039/501100001871 |
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