Extraction and characterization of Argentine red shrimp (Pleoticus muelleri) phospholipids as raw material for liposome production
Phospholipids rich in omega-3 fatty acids from Argentine red shrimp waste were explored as a source to produce food-grade liposomes. Partially purified phospholipids (PL-AS), hexane-soluble (HxSE) and acetone-soluble (Ac-SE) lipid co-extracts, were characterized in terms of extraction yield (2.0%, 1.46% and 4.51%, respectively), chemical composition (fatty acids, tocopherols, sterols, astaxanthin) and thermal stability. Based on lipid fractionation, PL-AS presented 85% phospholipids, while neutral lipids were mostly present in HxSE (75%) and free FA in AcSE (34%), the latter suggesting significant fat hydrolysis. Palmitic, oleic, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids predominated in the phospholipid fraction of PL-AS, mainly constituted by phosphatidylcholine (PC) (96%). The most abundant phospholipid was identified at m/z 760.59, composed of PC, with C16:0/C18:1 as the most probable FA combination. Unilamellar spherical liposomes were successfully made of PL-AS (≈140 nm, 0.248 PDI, −68.5 mV ζ potential), showing high stability for 28 days at 4 °C.
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2022
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| Subjects: | Liposomes, Phospholipids, Shrimp waste, Omega-3 fatty acids, DSC, Morphology, |
| Online Access: | http://hdl.handle.net/10261/255812 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100003339 |
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