Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes
13 pages, 3 figures, 1 table, supporting information https://doi.org/10.1111/1462-2920.16337.-- Data Availability Statement: The raw, demultiplexed fastq files were deposited at the European Nucleotide Archive (ENA) database under study accession numbers PRJEB47424, PRJEB47446, PRJEB47390, PRJEB47406, and PRJEB47407 for the data sets of amoA of comammox Nitrospira from seasonal samples, nxrB of canonical and comammox Nitrospira from seasonal samples, amoA of comammox Nitrospira from the pH-controlled incubations, nxrB of canonical and comammox Nitrospira from the pH-controlled incubations, and amoA of Nitrososphaerales from the pH-controlled incubations, respectively
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John Wiley & Sons
2023-05
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| Subjects: | Conserve and sustainably use the oceans, seas and marine resources for sustainable development, |
| Online Access: | http://hdl.handle.net/10261/330828 |
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dig-icm-es-10261-3308282023-07-20T11:15:56Z Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes Daebeler, Anne Güell-Bujons, Queralt Mooshammer, Maria Zechmeister, Thomas Herbold, Craig W. Richter, Andreas Wagner, Michael Daims, Holger Austrian Science Fund Czech Science Foundation European Research Council European Commission Agencia Estatal de Investigación (España) Conserve and sustainably use the oceans, seas and marine resources for sustainable development 13 pages, 3 figures, 1 table, supporting information https://doi.org/10.1111/1462-2920.16337.-- Data Availability Statement: The raw, demultiplexed fastq files were deposited at the European Nucleotide Archive (ENA) database under study accession numbers PRJEB47424, PRJEB47446, PRJEB47390, PRJEB47406, and PRJEB47407 for the data sets of amoA of comammox Nitrospira from seasonal samples, nxrB of canonical and comammox Nitrospira from seasonal samples, amoA of comammox Nitrospira from the pH-controlled incubations, nxrB of canonical and comammox Nitrospira from the pH-controlled incubations, and amoA of Nitrososphaerales from the pH-controlled incubations, respectively Nitrite-oxidizing bacteria (NOB) catalyse the second nitrification step and are the main biological source of nitrate. The most diverse and widespread NOB genus is Nitrospira, which also contains complete ammonia oxidizers (comammox) that oxidize ammonia to nitrate. To date, little is known about the occurrence and biology of comammox and canonical nitrite oxidizing Nitrospira in extremely alkaline environments. Here, we studied the seasonal distribution and diversity, and the effect of short-term pH changes on comammox and canonical Nitrospira in sediments of two saline, highly alkaline lakes. We identified diverse canonical and comammox Nitrospira clade A-like phylotypes as the only detectable NOB during more than a year, suggesting their major importance for nitrification in these habitats. Gross nitrification rates measured in microcosm incubations were highest at pH 10 and considerably faster than reported for other natural, aquatic environments. Nitrification could be attributed to canonical and comammox Nitrospira and to Nitrososphaerales ammonia-oxidizing archaea. Furthermore, our data suggested that comammox Nitrospira contributed to ammonia oxidation at an extremely alkaline pH of 11. These results identify saline, highly alkaline lake sediments as environments of uniquely strong nitrification with novel comammox Nitrospira as key microbial players This research was supported by the Austrian Science Fund (FWF) grants T938 (to Anne Daebeler), P25231-B21 and P30570-B29 (both to Holger Daims), and the Czech Science Foundation (GACR) grant 21-17322M (to Anne Daebeler). Queralt Güell-Bujons was supported with a pre-doctoral contract by European Research Council Advanced Grant (ERC-2018-ADG-834162) under the EUs Horizon H2020 research and innovation program. Additionally, Queralt Güell-Bujons acknowledges the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) to the ICM Peer reviewed 2023-07-11T10:08:13Z 2023-07-11T10:08:13Z 2023-05 artículo Environmental Microbiology 25(5): 1055-1067 (2023) 1462-2912 CEX2019-000928-S http://hdl.handle.net/10261/330828 10.1111/1462-2920.16337 1462-2920 en Publisher's version https://doi.org/10.1111/1462-2920.16337 Sí open John Wiley & Sons |
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Conserve and sustainably use the oceans, seas and marine resources for sustainable development Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
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Conserve and sustainably use the oceans, seas and marine resources for sustainable development Conserve and sustainably use the oceans, seas and marine resources for sustainable development Daebeler, Anne Güell-Bujons, Queralt Mooshammer, Maria Zechmeister, Thomas Herbold, Craig W. Richter, Andreas Wagner, Michael Daims, Holger Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes |
| description |
13 pages, 3 figures, 1 table, supporting information https://doi.org/10.1111/1462-2920.16337.-- Data Availability Statement: The raw, demultiplexed fastq files were deposited at the European Nucleotide Archive (ENA) database under study accession numbers PRJEB47424, PRJEB47446, PRJEB47390, PRJEB47406, and PRJEB47407 for the data sets of amoA of comammox Nitrospira from seasonal samples, nxrB of canonical and comammox Nitrospira from seasonal samples, amoA of comammox Nitrospira from the pH-controlled incubations, nxrB of canonical and comammox Nitrospira from the pH-controlled incubations, and amoA of Nitrososphaerales from the pH-controlled incubations, respectively |
| author2 |
Austrian Science Fund |
| author_facet |
Austrian Science Fund Daebeler, Anne Güell-Bujons, Queralt Mooshammer, Maria Zechmeister, Thomas Herbold, Craig W. Richter, Andreas Wagner, Michael Daims, Holger |
| format |
artículo |
| topic_facet |
Conserve and sustainably use the oceans, seas and marine resources for sustainable development |
| author |
Daebeler, Anne Güell-Bujons, Queralt Mooshammer, Maria Zechmeister, Thomas Herbold, Craig W. Richter, Andreas Wagner, Michael Daims, Holger |
| author_sort |
Daebeler, Anne |
| title |
Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes |
| title_short |
Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes |
| title_full |
Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes |
| title_fullStr |
Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes |
| title_full_unstemmed |
Rapid nitrification involving comammox and canonical Nitrospira at extreme pH in saline-alkaline lakes |
| title_sort |
rapid nitrification involving comammox and canonical nitrospira at extreme ph in saline-alkaline lakes |
| publisher |
John Wiley & Sons |
| publishDate |
2023-05 |
| url |
http://hdl.handle.net/10261/330828 |
| work_keys_str_mv |
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| _version_ |
1777667990000500736 |