Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography
13 pages, 6 figures, 4 tables
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| Format: | artículo biblioteca |
| Language: | English |
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Inter Research
2006-12-21
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| Subjects: | Reservoir bacteria, Resource availability, Bacterivory, Leucine incorporation, Bacterioplankton structure, Bacterioplankton function, |
| Online Access: | http://hdl.handle.net/10261/27412 |
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dig-icm-es-10261-274122020-11-12T09:28:13Z Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography Hornák, Karel Jezbera, Jan Nedoma, Jirí Gasol, Josep M. Šimek, Karel Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function 13 pages, 6 figures, 4 tables We examined changes in the phylogenetic structure and leucine incorporation of bacterioplankton assemblages exposed to variable levels of available resources and bacterivory in a freshwater reservoir. Fluorescence in situ hybridization (FISH) combined with microautoradiography was used to determine bacterial phylogenetic affiliations and the proportions of cells incorporating leucine. Grazing-free and grazing-enhanced treatments were incubated in both P-limited and P-rich areas of the reservoir using dialysis bags, and bottle treatments with and without P enrichment. β-Proteobacteria, mainly the R-BT065 cluster (subcluster of Rhodoferax sp. BAL47), became the dominant leucine-active bacteria in all treatments. Proportions of active cells belonging to R-BT065 phylotypes considerably increased under conditions of enhanced protistan grazing and phosphorus availability. In contrast, proportions of cells from the Sphingobacteria/Flavobacteria group usually decreased and exhibited very low rates of leucine uptake. γ-Proteobacteria was the least abundant but nevertheless a highly active part of the assemblage. Phosphorus addition supported incorporation of leucine by all bacterial groups. Although highly vulnerable to predation, only R-BT065 cells exhibited marked increases in both relative abundance within the bacterial community and in proportions of active cells. The phylogenetic composition and leucine-uptake of bacterioplankton was more markedly affected by resource availability than by grazing pressure, which only had a strong effect on the dynamics of R-BT065 bacteria within the P-limited area of the reservoir This study was supported by the Grant Agency of the Czech Republic (research grant 206/05/0007 awarded to K.2., and FRV2 grant 1062/2004 awarded to K.H.), and by the Academy of Sciences of the Czech Republic project AV0Z 60170517 Peer reviewed 2010-09-03T09:11:27Z 2010-09-03T09:11:27Z 2006-12-21 artículo http://purl.org/coar/resource_type/c_6501 Aquatic Microbial Ecology 45(3): 277-289 (2006) 0948-3055 http://hdl.handle.net/10261/27412 10.3354/ame045277 1616-1564 en https://doi.org/10.3354/ame045277 open 5867 bytes application/pdf Inter Research |
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biblioteca |
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Biblioteca del ICM España |
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English |
| topic |
Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function |
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Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Hornák, Karel Jezbera, Jan Nedoma, Jirí Gasol, Josep M. Šimek, Karel Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| description |
13 pages, 6 figures, 4 tables |
| format |
artículo |
| topic_facet |
Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function Reservoir bacteria Resource availability Bacterivory Leucine incorporation Bacterioplankton structure Bacterioplankton function |
| author |
Hornák, Karel Jezbera, Jan Nedoma, Jirí Gasol, Josep M. Šimek, Karel |
| author_facet |
Hornák, Karel Jezbera, Jan Nedoma, Jirí Gasol, Josep M. Šimek, Karel |
| author_sort |
Hornák, Karel |
| title |
Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| title_short |
Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| title_full |
Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| title_fullStr |
Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| title_full_unstemmed |
Effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| title_sort |
effects of resource availability and bacterivory on leucine incorporation in different groups of freshwater bacterioplankton, assessed using microautoradiography |
| publisher |
Inter Research |
| publishDate |
2006-12-21 |
| url |
http://hdl.handle.net/10261/27412 |
| work_keys_str_mv |
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| _version_ |
1777665765814566912 |