Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus
10 pages, 6 figures, 2 tables
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Society for the Study of Reproduction
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| Subjects: | Cathepsins, Developmental biology, Free amino acids, Gamete biology, Gametogenesis, Hydration, Killifish, Maturation-inducing steroid, Oocyte development, Proteolysis, |
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dig-icm-es-10261-211622020-11-10T09:59:54Z Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus LaFleur, Gary J. Raldúa, Demetrio Fabra, Mercedes Carnevali, Oliana Denslow, Nancy Wallace, Robin A. Cerdà, Joan Cathepsins Developmental biology Free amino acids Gamete biology Gametogenesis Hydration Killifish Maturation-inducing steroid Oocyte development Proteolysis 10 pages, 6 figures, 2 tables Various Coomassie blue-staining yolk proteins (YPs) present in oocytes and eggs of Fundulus heteroclitus, a teleost that produces low hydrated, demersal eggs (benthophil species), were subjected to N-terminal microsequencing. Four YPs were N-terminally blocked, while five yielded sequence information. Of the latter, four corresponded to internal sequences of vitellogenin 1 (Vg1), whereas a fifth band corresponded to the N-terminal sequence of Vg2. Phosphorylated YPs (phosvitins and phosvettes) derived from the polyserine domain of Vg were not successfully sequenced. The major N-terminally blocked 122- and 103-kDa YPs both represented the lipovitellin heavy chain of Vg1 (LvH1), and thus most of the oocyte YPs were derived from Vg1. During oocyte maturation in vivo and in vitro, the LvH1 122 is degraded, concomitant with an increased enzymatic activity of cathepsin B, while the 45-kDa YP is converted to a 42-kDa YP. The LvH1 122 was found to contain a consensus site for proteolytic degradation (PEST) near its C-terminus, which is missing from its stable, but truncated twin sequence, LvH1 103. We suggest that this site becomes exposed to cathepsin B during the hydration process that accompanies oocyte maturation and renders the LvH1 122 susceptible to proteolysis. PEST sites are found in Vg sequences from other benthophil fish, whereas, interestingly, they are missing in marine teleosts that spawn highly hydrated, pelagic eggs (pelagophil species), displaying a different pattern of Vg incorporation into YPs and LvH1 and LvH2 processing to that found in F. heteroclitus. Thus, different models of Vg/YP precursor/product relationship and further processing during oocyte maturation and hydration are proposed for pelagophil and benthophil teleosts. Supported by the Division of Sponsored Research, University of Florida, through National Science Foundation grant IBN-9306123 to R.A.W., and by grant AGL2001-0364/ACU from the Spanish Ministry of Science and Technology to J.C., who was also supported by the Reference Center in Aquaculture (Generalitat de Catalunya), Spain. M.F. was recipient of a fellowship from the Catalan Government (DURSI). Peer reviewed 2005-10 artículo http://purl.org/coar/resource_type/c_6501 Biology of Reproduction 73(4): 815-824 (2005) 0006-3363 10261/21162 10.1095/biolreprod.105.041335 en https://doi.org/10.1095/biolreprod.105.041335 none Society for the Study of Reproduction |
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Cathepsins Developmental biology Free amino acids Gamete biology Gametogenesis Hydration Killifish Maturation-inducing steroid Oocyte development Proteolysis Cathepsins Developmental biology Free amino acids Gamete biology Gametogenesis Hydration Killifish Maturation-inducing steroid Oocyte development Proteolysis |
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Cathepsins Developmental biology Free amino acids Gamete biology Gametogenesis Hydration Killifish Maturation-inducing steroid Oocyte development Proteolysis Cathepsins Developmental biology Free amino acids Gamete biology Gametogenesis Hydration Killifish Maturation-inducing steroid Oocyte development Proteolysis LaFleur, Gary J. Raldúa, Demetrio Fabra, Mercedes Carnevali, Oliana Denslow, Nancy Wallace, Robin A. Cerdà, Joan Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus |
| description |
10 pages, 6 figures, 2 tables |
| format |
artículo |
| topic_facet |
Cathepsins Developmental biology Free amino acids Gamete biology Gametogenesis Hydration Killifish Maturation-inducing steroid Oocyte development Proteolysis |
| author |
LaFleur, Gary J. Raldúa, Demetrio Fabra, Mercedes Carnevali, Oliana Denslow, Nancy Wallace, Robin A. Cerdà, Joan |
| author_facet |
LaFleur, Gary J. Raldúa, Demetrio Fabra, Mercedes Carnevali, Oliana Denslow, Nancy Wallace, Robin A. Cerdà, Joan |
| author_sort |
LaFleur, Gary J. |
| title |
Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus |
| title_short |
Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus |
| title_full |
Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus |
| title_fullStr |
Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus |
| title_full_unstemmed |
Derivation of Major Yolk Proteins from Parental Vitellogenins and Alternative Processing During Oocyte Maturation in Fundulus heteroclitus |
| title_sort |
derivation of major yolk proteins from parental vitellogenins and alternative processing during oocyte maturation in fundulus heteroclitus |
| publisher |
Society for the Study of Reproduction |
| work_keys_str_mv |
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