Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)

Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)

9 pages, 2 figures, 3 tables

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Bibliographic Details
Main Authors: Castro, Jaime, Bouza, Carmen, Sánchez, Laura, Cal, Rosa, Piferrer, Francesc, Martínez, Paulino
Format: artículo biblioteca
Language:English
Published: Springer 2003-10-23
Subjects:Gynogenesis, Microsatellites, Turbot, Nucleolus organizer regions,
Online Access:http://hdl.handle.net/10261/16824
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spelling dig-icm-es-10261-168242020-11-26T12:39:05Z Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus) Castro, Jaime Bouza, Carmen Sánchez, Laura Cal, Rosa Piferrer, Francesc Martínez, Paulino Gynogenesis Microsatellites Turbot Nucleolus organizer regions 9 pages, 2 figures, 3 tables Gynogenesis was assessed by different methods in 2 families of gynogenetic offspring in turbot (Scophthalmus maximus). Karyotype analysis in embryos and larvae demonstrated high accuracy in estimation of ploidy level, but performance was uneven given the low quality and number of plates obtained. The use of silver staining to estimate the number of nucleoli per nucleus resulted in a straightforward and easy method to evaluate the ploidy of the samples studied. However, the existence of a nucleolus organizer region polymorphism in turbot determined a small error in ploidy estimation, important when checking ploidy in specific individuals. The use of a set of 11 highly variable microsatellite loci proved to be a powerful method to confirm the exclusive maternal inheritance to gynogenetic offspring in turbot, with probabilities of detection of putative paternal genetic contribution above 99.99% This work has been supported by FEDER grant 1FD1997-2404 Peer reviewed 2009-09-10T09:48:14Z 2009-09-10T09:48:14Z 2003-10-23 artículo http://purl.org/coar/resource_type/c_6501 Marine Biotechnology 5(6): 584-592 (2003) 1436-2228 http://hdl.handle.net/10261/16824 10.1007/s10126-003-0004-x 1436-2236 en https://doi.org/10.1007/s10126-003-0004-x none 918459 bytes application/pdf Springer
institution ICM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-icm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del ICM España
language English
topic Gynogenesis
Microsatellites
Turbot
Nucleolus organizer regions
Gynogenesis
Microsatellites
Turbot
Nucleolus organizer regions
spellingShingle Gynogenesis
Microsatellites
Turbot
Nucleolus organizer regions
Gynogenesis
Microsatellites
Turbot
Nucleolus organizer regions
Castro, Jaime
Bouza, Carmen
Sánchez, Laura
Cal, Rosa
Piferrer, Francesc
Martínez, Paulino
Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)
description 9 pages, 2 figures, 3 tables
format artículo
topic_facet Gynogenesis
Microsatellites
Turbot
Nucleolus organizer regions
author Castro, Jaime
Bouza, Carmen
Sánchez, Laura
Cal, Rosa
Piferrer, Francesc
Martínez, Paulino
author_facet Castro, Jaime
Bouza, Carmen
Sánchez, Laura
Cal, Rosa
Piferrer, Francesc
Martínez, Paulino
author_sort Castro, Jaime
title Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)
title_short Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)
title_full Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)
title_fullStr Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)
title_full_unstemmed Gynogenesis assessment using microsatellite genetic markers in turbot ( Scophthalmus maximus)
title_sort gynogenesis assessment using microsatellite genetic markers in turbot ( scophthalmus maximus)
publisher Springer
publishDate 2003-10-23
url http://hdl.handle.net/10261/16824
work_keys_str_mv AT castrojaime gynogenesisassessmentusingmicrosatellitegeneticmarkersinturbotscophthalmusmaximus
AT bouzacarmen gynogenesisassessmentusingmicrosatellitegeneticmarkersinturbotscophthalmusmaximus
AT sanchezlaura gynogenesisassessmentusingmicrosatellitegeneticmarkersinturbotscophthalmusmaximus
AT calrosa gynogenesisassessmentusingmicrosatellitegeneticmarkersinturbotscophthalmusmaximus
AT piferrerfrancesc gynogenesisassessmentusingmicrosatellitegeneticmarkersinturbotscophthalmusmaximus
AT martinezpaulino gynogenesisassessmentusingmicrosatellitegeneticmarkersinturbotscophthalmusmaximus
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