Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon
7 pages, figures, and tables statistics.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
1988
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| Subjects: | Lolium rigidum gaud, Catalase, Cotyledon leaf, Diamino benzidine (DAB), Enzyme histochemistry, Herbicide, Peroxidase, Peroxisomes, |
| Online Access: | http://hdl.handle.net/10261/35803 |
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dig-ica-es-10261-358032016-05-04T07:20:39Z Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon Felipe, Mª Rosario de Lucas, M. Mercedes Pozuelo, José Manuel Lolium rigidum gaud Catalase Cotyledon leaf Diamino benzidine (DAB) Enzyme histochemistry Herbicide Peroxidase Peroxisomes 7 pages, figures, and tables statistics. A study was made of the number of peroxisomes appearing m the mesophyll of Lolium rigidum in control and isoproturon-treated plants and also, using the diamino benzidme (DAB) technique, the cytochemical localization of catalase and peroxidase enzymes in the same tissues. The number of peroxisomes increased when growing in the presence of a 3.4x 10~5M isoproturon concentration. Isoproturon also somewhat changed the normal position of the peroxisomes. With regard to the cytochemical results, DAB precipítate (osmium black reaction product) was located mainly in the cell wall, vacuole membrane, and in the chloroplasts of the isoproturon-treated mesophyll. However, there was a decrease in DAB m the microbodies of these treated plants. In this study, microbody-like structures associated with a kind of network were observed, and we believe these to be microbodies in development, the Golgi vesicles contributing to their formation. They also presented DAB precipítate in their small internal compartments, probably corresponding to catalase activity. The results are discussed in relation to the senescence of the tissues studied. Wegratefully acknowledge the expert technical assistance of Mrs.Carmen Mesa and M. Luisa Melendo and to the Electron Microscopy Laboratory for their facilities. The research was supported by funds from CAICYT, Spain. Peer reviewed 2011-05-18T11:50:33Z 2011-05-18T11:50:33Z 1988 artículo http://purl.org/coar/resource_type/c_6501 Journal of pplant physiology 132: 67-73 (1988) 0176-1617 http://hdl.handle.net/10261/35803 en none Elsevier |
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English |
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Lolium rigidum gaud Catalase Cotyledon leaf Diamino benzidine (DAB) Enzyme histochemistry Herbicide Peroxidase Peroxisomes Lolium rigidum gaud Catalase Cotyledon leaf Diamino benzidine (DAB) Enzyme histochemistry Herbicide Peroxidase Peroxisomes |
| spellingShingle |
Lolium rigidum gaud Catalase Cotyledon leaf Diamino benzidine (DAB) Enzyme histochemistry Herbicide Peroxidase Peroxisomes Lolium rigidum gaud Catalase Cotyledon leaf Diamino benzidine (DAB) Enzyme histochemistry Herbicide Peroxidase Peroxisomes Felipe, Mª Rosario de Lucas, M. Mercedes Pozuelo, José Manuel Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon |
| description |
7 pages, figures, and tables statistics. |
| format |
artículo |
| topic_facet |
Lolium rigidum gaud Catalase Cotyledon leaf Diamino benzidine (DAB) Enzyme histochemistry Herbicide Peroxidase Peroxisomes |
| author |
Felipe, Mª Rosario de Lucas, M. Mercedes Pozuelo, José Manuel |
| author_facet |
Felipe, Mª Rosario de Lucas, M. Mercedes Pozuelo, José Manuel |
| author_sort |
Felipe, Mª Rosario de |
| title |
Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon |
| title_short |
Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon |
| title_full |
Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon |
| title_fullStr |
Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon |
| title_full_unstemmed |
Cytochemical Study of Catalase and Peroxidase in the Mesophyll of Lolium rigidum Plants Treated with Isoproturon |
| title_sort |
cytochemical study of catalase and peroxidase in the mesophyll of lolium rigidum plants treated with isoproturon |
| publisher |
Elsevier |
| publishDate |
1988 |
| url |
http://hdl.handle.net/10261/35803 |
| work_keys_str_mv |
AT felipemarosariode cytochemicalstudyofcatalaseandperoxidaseinthemesophyllofloliumrigidumplantstreatedwithisoproturon AT lucasmmercedes cytochemicalstudyofcatalaseandperoxidaseinthemesophyllofloliumrigidumplantstreatedwithisoproturon AT pozuelojosemanuel cytochemicalstudyofcatalaseandperoxidaseinthemesophyllofloliumrigidumplantstreatedwithisoproturon |
| _version_ |
1777663593822552064 |