Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds
8 pages, figures, and tables statistics.
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| Format: | artículo biblioteca |
| Language: | English |
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Springer
2001
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| Subjects: | Alkaloids, Lupinus spp, Immunocytochemistry, X-ray microanalysis, Protein bodies, |
| Online Access: | http://hdl.handle.net/10261/29696 |
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dig-ica-es-10261-296962021-06-07T07:41:09Z Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds Pozuelo, José Manuel Lucas, M. Mercedes Lorenzo, Cristina de Fernández-Pascual, Mercedes Maldonado, Susana Felipe, Mª Rosario de Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies 8 pages, figures, and tables statistics. Immunolocalization of alkaloids in lupin seeds (Lupinus spp.) has been performed by cryofixation and conventional methods. Alkaloids were localized in the protein bodies of the cotyledon ceils. Some immunogold particles in the walls of these cells were also observed. There were no differences in the sites of localization between the two mentioned methods. X-ray microanalysis of elements showed the presence of R Mg, S, and K in the protein bodies of cotyledon cells in lupin seeds. The role of K + in alkaloids transport is discussed. We greatfully acknowledge Dr. Greirson's kind supply of the antibody for alkaloids localization. The technical assistance of C. de Mesa, M. L. Melendo, and M. I. Mendndez is also greatly appreciated, as well as the assistance from Mr. F. Pinto for the electron microscopy facilities. Peer reviewed 2010-12-01T09:03:56Z 2010-12-01T09:03:56Z 2001 artículo http://purl.org/coar/resource_type/c_6501 Protoplasma 218:104-111 (2001) 0033-183x http://hdl.handle.net/10261/29696 10.1007/BF01288366 en https://doi.org/10.1007/BF01288366 none Springer |
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ES |
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| databasecode |
dig-ica-es |
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biblioteca |
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English |
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Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies |
| spellingShingle |
Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies Pozuelo, José Manuel Lucas, M. Mercedes Lorenzo, Cristina de Fernández-Pascual, Mercedes Maldonado, Susana Felipe, Mª Rosario de Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
| description |
8 pages, figures, and tables statistics. |
| format |
artículo |
| topic_facet |
Alkaloids Lupinus spp Immunocytochemistry X-ray microanalysis Protein bodies |
| author |
Pozuelo, José Manuel Lucas, M. Mercedes Lorenzo, Cristina de Fernández-Pascual, Mercedes Maldonado, Susana Felipe, Mª Rosario de |
| author_facet |
Pozuelo, José Manuel Lucas, M. Mercedes Lorenzo, Cristina de Fernández-Pascual, Mercedes Maldonado, Susana Felipe, Mª Rosario de |
| author_sort |
Pozuelo, José Manuel |
| title |
Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
| title_short |
Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
| title_full |
Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
| title_fullStr |
Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
| title_full_unstemmed |
Immunolocalization of alkaloids and X-ray microanalysis of elements in lupin seeds |
| title_sort |
immunolocalization of alkaloids and x-ray microanalysis of elements in lupin seeds |
| publisher |
Springer |
| publishDate |
2001 |
| url |
http://hdl.handle.net/10261/29696 |
| work_keys_str_mv |
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| _version_ |
1777663585843937280 |