A new method for microwave assisted ethanolic extraction of Mentha rotundifolia bioactive terpenoids
A new microwave-assisted extraction (MAE) method using ethanol as solvent has been optimized by means of a Box-Behnken experimental design for the enhanced extraction of bioactive terpenoids from Mentha rotundifolia leaves; 100°C, 5 min, 1.125 g dry sample: 10 mL solvent and a single extraction cycle were selected as optimal conditions. Improved performance of MAE method in terms of extraction yield and/or reproducibility over conventional solid-liquid extraction and ultrasound assisted extraction was also previously assessed. A comprehensive characterization of MAE extracts was carried out by GC-MS. A total of 46 compounds, mostly terpenoids, were identified; piperitenone oxide and piperitenone were the major compounds determined. Several neophytadiene isomers were also detected for the first time in MAE extracts. Different procedures (solid-phase extraction and activated charcoal (AC) treatment) were also evaluated for clean-up of MAE extracts, with AC providing the highest enrichment in bioactive terpenoids. Finally, the MAE method here developed is shown as a green, fast, efficient and reproducible liquid extraction methodology to obtain M. rotundifolia bioactive extracts for further application, among others, as food preservatives.
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Published: |
John Wiley & Sons
2018
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| Subjects: | Gas chromatography-mass spectrometry, Mentha rotundifolia, Microwave assisted extraction, Terpenoids, |
| Online Access: | http://hdl.handle.net/10261/172419 http://dx.doi.org/10.13039/100008054 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100010198 |
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