A phylogenomic resolution for the taxonomy of Aegean green lizards
Lacerta pamphylica and Lacerta trilineata are two currently recognized green lizard species with a historically problematic taxonomy. In cases of tangled phylogenies, next-generation sequencing and double-digest restriction-site-associated DNA protocols can provide a wealth of genomic data and resolve difficult taxonomic issues. Here, we generated genome-wide SNPs and mitochondrial sequences, and applied molecular species delimitation approaches to provide a stable taxonomy for the Aegean green lizards. Mitochondrial gene trees, genetic cluster delimitation and population structure analyses converged into recognizing the populations of (a) L. pamphylica, (b) east Aegean islands, Anatolia and Thrace (diplochondrodes lineage), (c) central Aegean islands (citrovittata), and (d) remaining Balkan populations and islands (trilineata), as separate clusters. Phylogenomic analyses revealed a split into two major clades, east and west of the Aegean Barrier, unambiguously showing a sister–clade relationship between pamphylica and diplochondrodes, rendering L. trilineata paraphyletic. Species delimitation models were tested in a Bayesian framework using the genomic SNPs: lumping all populations into a single ‘species’ had the lowest likelihood but the current taxonomy was also outperformed by all other models. All lines of evidence support the Pamphylian green lizard as a valid species; thus, east Aegean L. trilineata should also be considered a distinct species under the name Lacerta diplochondrodes. Finally, evidence from the mitochondrial and nuclear genomes is overwhelmingly in favour of recognizing the morphologically distinct Cycladian green lizards as a distinct species. We propose their elevation to full species under the name Lacerta citrovittata. All remaining insular and continental populations of the Balkan Peninsula represent the species L. trilineata.
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John Wiley & Sons
2020-01
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Subjects: | ddRAD, Lacertidae, Mediterranean, Molecular systematics, Taxonomy, |
Online Access: | http://hdl.handle.net/10261/207679 http://dx.doi.org/10.13039/100000002 |
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dig-ibe-es-10261-2076792020-12-13T00:10:14Z A phylogenomic resolution for the taxonomy of Aegean green lizards Kornilios, Panagiotis Thanou, Evanthia Lymberakis, Petros Ilgaz, Çetin Kumlutas, Yusuf Leaché, Adam National Institutes of Health (US) ddRAD Lacertidae Mediterranean Molecular systematics Taxonomy Lacerta pamphylica and Lacerta trilineata are two currently recognized green lizard species with a historically problematic taxonomy. In cases of tangled phylogenies, next-generation sequencing and double-digest restriction-site-associated DNA protocols can provide a wealth of genomic data and resolve difficult taxonomic issues. Here, we generated genome-wide SNPs and mitochondrial sequences, and applied molecular species delimitation approaches to provide a stable taxonomy for the Aegean green lizards. Mitochondrial gene trees, genetic cluster delimitation and population structure analyses converged into recognizing the populations of (a) L. pamphylica, (b) east Aegean islands, Anatolia and Thrace (diplochondrodes lineage), (c) central Aegean islands (citrovittata), and (d) remaining Balkan populations and islands (trilineata), as separate clusters. Phylogenomic analyses revealed a split into two major clades, east and west of the Aegean Barrier, unambiguously showing a sister–clade relationship between pamphylica and diplochondrodes, rendering L. trilineata paraphyletic. Species delimitation models were tested in a Bayesian framework using the genomic SNPs: lumping all populations into a single ‘species’ had the lowest likelihood but the current taxonomy was also outperformed by all other models. All lines of evidence support the Pamphylian green lizard as a valid species; thus, east Aegean L. trilineata should also be considered a distinct species under the name Lacerta diplochondrodes. Finally, evidence from the mitochondrial and nuclear genomes is overwhelmingly in favour of recognizing the morphologically distinct Cycladian green lizards as a distinct species. We propose their elevation to full species under the name Lacerta citrovittata. All remaining insular and continental populations of the Balkan Peninsula represent the species L. trilineata. This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 OD018174 Instrumentation Grant. 2020-04-15T12:19:24Z 2020-04-15T12:19:24Z 2020-01 2020-04-15T12:19:25Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1111/zsc.12385 e-issn: 1463-6409 issn: 0300-3256 Zoologica Scripta 49(1): 14-27 (2019) http://hdl.handle.net/10261/207679 10.1111/zsc.12385 http://dx.doi.org/10.13039/100000002 http://doi.org/10.1111/zsc.12385 Sí none John Wiley & Sons |
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ddRAD Lacertidae Mediterranean Molecular systematics Taxonomy ddRAD Lacertidae Mediterranean Molecular systematics Taxonomy Kornilios, Panagiotis Thanou, Evanthia Lymberakis, Petros Ilgaz, Çetin Kumlutas, Yusuf Leaché, Adam A phylogenomic resolution for the taxonomy of Aegean green lizards |
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Lacerta pamphylica and Lacerta trilineata are two currently recognized green lizard species with a historically problematic taxonomy. In cases of tangled phylogenies, next-generation sequencing and double-digest restriction-site-associated DNA protocols can provide a wealth of genomic data and resolve difficult taxonomic issues. Here, we generated genome-wide SNPs and mitochondrial sequences, and applied molecular species delimitation approaches to provide a stable taxonomy for the Aegean green lizards. Mitochondrial gene trees, genetic cluster delimitation and population structure analyses converged into recognizing the populations of (a) L. pamphylica, (b) east Aegean islands, Anatolia and Thrace (diplochondrodes lineage), (c) central Aegean islands (citrovittata), and (d) remaining Balkan populations and islands (trilineata), as separate clusters. Phylogenomic analyses revealed a split into two major clades, east and west of the Aegean Barrier, unambiguously showing a sister–clade relationship between pamphylica and diplochondrodes, rendering L. trilineata paraphyletic. Species delimitation models were tested in a Bayesian framework using the genomic SNPs: lumping all populations into a single ‘species’ had the lowest likelihood but the current taxonomy was also outperformed by all other models. All lines of evidence support the Pamphylian green lizard as a valid species; thus, east Aegean L. trilineata should also be considered a distinct species under the name Lacerta diplochondrodes. Finally, evidence from the mitochondrial and nuclear genomes is overwhelmingly in favour of recognizing the morphologically distinct Cycladian green lizards as a distinct species. We propose their elevation to full species under the name Lacerta citrovittata. All remaining insular and continental populations of the Balkan Peninsula represent the species L. trilineata. |
author2 |
National Institutes of Health (US) |
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National Institutes of Health (US) Kornilios, Panagiotis Thanou, Evanthia Lymberakis, Petros Ilgaz, Çetin Kumlutas, Yusuf Leaché, Adam |
format |
artículo |
topic_facet |
ddRAD Lacertidae Mediterranean Molecular systematics Taxonomy |
author |
Kornilios, Panagiotis Thanou, Evanthia Lymberakis, Petros Ilgaz, Çetin Kumlutas, Yusuf Leaché, Adam |
author_sort |
Kornilios, Panagiotis |
title |
A phylogenomic resolution for the taxonomy of Aegean green lizards |
title_short |
A phylogenomic resolution for the taxonomy of Aegean green lizards |
title_full |
A phylogenomic resolution for the taxonomy of Aegean green lizards |
title_fullStr |
A phylogenomic resolution for the taxonomy of Aegean green lizards |
title_full_unstemmed |
A phylogenomic resolution for the taxonomy of Aegean green lizards |
title_sort |
phylogenomic resolution for the taxonomy of aegean green lizards |
publisher |
John Wiley & Sons |
publishDate |
2020-01 |
url |
http://hdl.handle.net/10261/207679 http://dx.doi.org/10.13039/100000002 |
work_keys_str_mv |
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