Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells
During insect oogenesis, the follicular epithelium undergoes both cell proliferation and apoptosis, thus modulating ovarian follicle growth. The Hippo pathway is key in these processes, and has been thoroughly studied in the meroistic ovaries of Drosophila melanogaster. However, nothing is known about the role of the Hippo pathway in primitive panoistic ovaries. This work examines the mRNA expression levels of the main components of the Hippo pathway in the panoistic ovary of the basal insect species Blattella germanica, and demonstrates the function of Hippo through RNAi. In Hippo-depleted specimens, the follicular cells of the basal ovarian follicles proliferate without arresting cytokinesis; the epithelium therefore becomes bilayered, impairing ovarian follicle growth. This phenotype is accompanied by long stalks between the ovarian follicles. In D. melanogaster loss of function of Notch determines that the stalk is not developed. With this in mind, we tested whether Hippo and Notch pathways are related in B. germanica. In Notch (only)-depleted females, no stalks were formed between the ovarian follicles. Simultaneous depletion of Hippo and Notch rescued partially the stalk to wild-type. Unlike in the meroistic ovaries of D. melanogaster, in panoistic ovaries the Hippo pathway appears to regulate follicular cell proliferation by acting as a repressor of Notch, triggering the switch from mitosis to the endocycle in the follicular cells. The phylogenetically basal position of B. germanica suggests that this might be the ancestral function of Hippo in insect ovaries. Copyright: © 2014 Irles, Piulachs.
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dig-ibe-es-10261-1157472021-12-28T16:50:46Z Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells Irles, Paula Piulachs, Maria-Dolors During insect oogenesis, the follicular epithelium undergoes both cell proliferation and apoptosis, thus modulating ovarian follicle growth. The Hippo pathway is key in these processes, and has been thoroughly studied in the meroistic ovaries of Drosophila melanogaster. However, nothing is known about the role of the Hippo pathway in primitive panoistic ovaries. This work examines the mRNA expression levels of the main components of the Hippo pathway in the panoistic ovary of the basal insect species Blattella germanica, and demonstrates the function of Hippo through RNAi. In Hippo-depleted specimens, the follicular cells of the basal ovarian follicles proliferate without arresting cytokinesis; the epithelium therefore becomes bilayered, impairing ovarian follicle growth. This phenotype is accompanied by long stalks between the ovarian follicles. In D. melanogaster loss of function of Notch determines that the stalk is not developed. With this in mind, we tested whether Hippo and Notch pathways are related in B. germanica. In Notch (only)-depleted females, no stalks were formed between the ovarian follicles. Simultaneous depletion of Hippo and Notch rescued partially the stalk to wild-type. Unlike in the meroistic ovaries of D. melanogaster, in panoistic ovaries the Hippo pathway appears to regulate follicular cell proliferation by acting as a repressor of Notch, triggering the switch from mitosis to the endocycle in the follicular cells. The phylogenetically basal position of B. germanica suggests that this might be the ancestral function of Hippo in insect ovaries. Copyright: © 2014 Irles, Piulachs. Peer reviewed 2015-05-26T09:22:29Z 2015-05-26T09:22:29Z 2014-11-26 artículo http://purl.org/coar/resource_type/c_6501 PLoS ONE 9(11): e113850 (2014) 1932-6203 http://hdl.handle.net/10261/115747 10.1371/journal.pone.0113850 25426635 en Publisher's version http://dx.doi.org/10.1371/journal.pone.0113850 Sí open Public Library of Science |
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During insect oogenesis, the follicular epithelium undergoes both cell proliferation and apoptosis, thus modulating ovarian follicle growth. The Hippo pathway is key in these processes, and has been thoroughly studied in the meroistic ovaries of Drosophila melanogaster. However, nothing is known about the role of the Hippo pathway in primitive panoistic ovaries. This work examines the mRNA expression levels of the main components of the Hippo pathway in the panoistic ovary of the basal insect species Blattella germanica, and demonstrates the function of Hippo through RNAi. In Hippo-depleted specimens, the follicular cells of the basal ovarian follicles proliferate without arresting cytokinesis; the epithelium therefore becomes bilayered, impairing ovarian follicle growth. This phenotype is accompanied by long stalks between the ovarian follicles. In D. melanogaster loss of function of Notch determines that the stalk is not developed. With this in mind, we tested whether Hippo and Notch pathways are related in B. germanica. In Notch (only)-depleted females, no stalks were formed between the ovarian follicles. Simultaneous depletion of Hippo and Notch rescued partially the stalk to wild-type. Unlike in the meroistic ovaries of D. melanogaster, in panoistic ovaries the Hippo pathway appears to regulate follicular cell proliferation by acting as a repressor of Notch, triggering the switch from mitosis to the endocycle in the follicular cells. The phylogenetically basal position of B. germanica suggests that this might be the ancestral function of Hippo in insect ovaries. Copyright: © 2014 Irles, Piulachs. |
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Irles, Paula Piulachs, Maria-Dolors |
spellingShingle |
Irles, Paula Piulachs, Maria-Dolors Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
author_facet |
Irles, Paula Piulachs, Maria-Dolors |
author_sort |
Irles, Paula |
title |
Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
title_short |
Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
title_full |
Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
title_fullStr |
Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
title_full_unstemmed |
Unlike in Drosophila meroistic ovaries, Hippo represses Notch in Blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
title_sort |
unlike in drosophila meroistic ovaries, hippo represses notch in blattella germanica panoistic ovaries, triggering the mitosis-endocycle switch in the follicular cells |
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Public Library of Science |
publishDate |
2014-11-26 |
url |
http://hdl.handle.net/10261/115747 |
work_keys_str_mv |
AT irlespaula unlikeindrosophilameroisticovarieshipporepressesnotchinblattellagermanicapanoisticovariestriggeringthemitosisendocycleswitchinthefollicularcells AT piulachsmariadolors unlikeindrosophilameroisticovarieshipporepressesnotchinblattellagermanicapanoisticovariestriggeringthemitosisendocycleswitchinthefollicularcells |
_version_ |
1777668616438677504 |