Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365
Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production.
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Language: | English |
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Elsevier
2024-05-01
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Subjects: | AmyR, Aspergillus vadensis, Gene regulation, Transcriptional activator, Genetic swaps, PrtT, Aspergillus, |
Online Access: | http://hdl.handle.net/10261/360502 https://api.elsevier.com/content/abstract/scopus_id/85192003746 |
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dig-iata-es-10261-3605022024-06-17T20:38:57Z Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 Liu, Dujuan Garrigues, Sandra Culleton, Helena McKie, Vincent A. de Vries, Ronald P. China Scholarship Council Dutch Research Council Garrigues, Sandra [0000-0002-4104-6246] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] AmyR Aspergillus vadensis Gene regulation Transcriptional activator Genetic swaps PrtT Aspergillus Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production. This research was supported by the China Scholarship Council for financial support (grant no. 202107720032). SG. was supported by a grant of the Applied Science Division (TTW) of NWO and the Biotechnology and Safety Program of the Ministry of Infrastructure and Water Management 15807 to RPdV. SG currently holds a Juan de la Cierva Incorporación grant (IJC 2020–042749-I) funded by MCIN/AEI/10.13039/501100011033 and the European Union Next Generation EU/PRTR. Peer reviewed 2024-06-17T06:26:55Z 2024-06-17T06:26:55Z 2024-05-01 artículo New Biotechnology 82: 25-32 (2024) 1871-6784 http://hdl.handle.net/10261/360502 10.1016/j.nbt.2024.04.003 38697469 2-s2.0-85192003746 https://api.elsevier.com/content/abstract/scopus_id/85192003746 en New biotechnology Publisher's version https://doi.org/10.1016/j.nbt.2024.04.003 Sí open Elsevier |
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AmyR Aspergillus vadensis Gene regulation Transcriptional activator Genetic swaps PrtT Aspergillus AmyR Aspergillus vadensis Gene regulation Transcriptional activator Genetic swaps PrtT Aspergillus |
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AmyR Aspergillus vadensis Gene regulation Transcriptional activator Genetic swaps PrtT Aspergillus AmyR Aspergillus vadensis Gene regulation Transcriptional activator Genetic swaps PrtT Aspergillus Liu, Dujuan Garrigues, Sandra Culleton, Helena McKie, Vincent A. de Vries, Ronald P. Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 |
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Aspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is primarily attributed to the lack of functionality of its gene's promoter sequence. In contrast, a different mechanism is likely causing the lack of PrtT activity, which is the main transcriptional regulator of protease production. The findings presented here not only expand our understanding of the genetic basis behind the distinct characteristics of A. vadensis CBS 113365, but also underscore its potential as a favourable alternative for recombinant protein production. |
author2 |
China Scholarship Council |
author_facet |
China Scholarship Council Liu, Dujuan Garrigues, Sandra Culleton, Helena McKie, Vincent A. de Vries, Ronald P. |
format |
artículo |
topic_facet |
AmyR Aspergillus vadensis Gene regulation Transcriptional activator Genetic swaps PrtT Aspergillus |
author |
Liu, Dujuan Garrigues, Sandra Culleton, Helena McKie, Vincent A. de Vries, Ronald P. |
author_sort |
Liu, Dujuan |
title |
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 |
title_short |
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 |
title_full |
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 |
title_fullStr |
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 |
title_full_unstemmed |
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365 |
title_sort |
analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of aspergillus vadensis cbs 113365 |
publisher |
Elsevier |
publishDate |
2024-05-01 |
url |
http://hdl.handle.net/10261/360502 https://api.elsevier.com/content/abstract/scopus_id/85192003746 |
work_keys_str_mv |
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