Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration

Pandiscia, Annamaria; Lorusso, Patrizio; Manfredi, Alessio; Sánchez Moragas, Gloria; Terio, Valentina; Randazzo, Walter; Ministerio de Ciencia, Innovación y Universidades (España)

Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration

© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Saved in:

Bibliographic Details
Main Authors:	Pandiscia, Annamaria, Lorusso, Patrizio, Manfredi, Alessio, Sánchez Moragas, Gloria, Terio, Valentina, Randazzo, Walter
Other Authors:	Ministerio de Ciencia, Innovación y Universidades (España)
Format:	artículo biblioteca
Language:	English
Published:	Multidisciplinary Digital Publishing Institute 2024-03-02
Subjects:	Norovirus, Vibrio, Bivalve mollusks, Inactivation, Food safety, Bacteriophages, Food processing technology, Water quality, Contamination,
Online Access:	http://hdl.handle.net/10261/352787 http://dx.doi.org/10.13039/501100005362 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837
Tags:	Add Tag No Tags, Be the first to tag this record!

id	dig-iata-es-10261-352787
record_format	koha
spelling	dig-iata-es-10261-3527872024-05-16T06:27:45Z Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration Pandiscia, Annamaria Lorusso, Patrizio Manfredi, Alessio Sánchez Moragas, Gloria Terio, Valentina Randazzo, Walter Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Università degli Studi di Bari Aldo Moro Ministerio de Ciencia e Innovación (España) Norovirus Vibrio Bivalve mollusks Inactivation Food safety Bacteriophages Food processing technology Water quality Contamination © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Cold plasma is a promising alternative for water treatment owing to pathogen control and a plethora of issues in the agriculture and food sectors. Shellfish pose a serious risk to public health and are linked to large viral and bacterial outbreaks. Hence, current European regulations mandate a depuration step for shellfish on the basis of their geographical growth area. This study investigated the inactivation of relevant viral and bacterial pathogens of three plasma-activated seawaters (PASWs), and their reactive oxygen and nitrogen species (RONS) composition, as being primarily responsible for microbial inactivation. Specifically, F-specific (MS2) and somatic (φ174) bacteriophage, cultivable surrogate (murine norovirus, MNV, and Tulane virus, TV), and human norovirus (HuNoV GII.4) inactivation was determined using plaque counts and infectivity assays, including the novel human intestinal enteroid (HIE) model for HuNoV. Moreover, the kinetic decay of Escherichia coli, Salmonella spp., and Vibrio parahaemolyticus was characterized. The results showed the complete inactivation of phages (6–8 log), surrogates (5–6 log), HuNoV (6 log), and bacterial (6–7 log) pathogens within 24 h while preventing cytotoxicity effects and preserving mussel viability. Nitrites (NO2−) were found to be mostly correlated with microbial decay. This research shows that PASWs are a suitable option to depurate bivalve mollusks and control the biohazard risk linked to their microbiological contamination, either viral or bacterial. This research was supported by the “PREVISION” (PID2019-105509RJ-I00, MCIN/AEI/10.13039/501100011033) project funded to W.R. A.M. was supported by a predoctoral research grant funded by the University of Bari “A. Moro” (Italy). IATA-CSIC is a Centre of Excellence Severo Ochoa (CEX2021-001189-S MCIN/AEI/10.13039/501100011033). With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S). Peer reviewed 2024-04-04T09:16:14Z 2024-04-04T09:16:14Z 2024-03-02 2024-04-04T09:16:14Z artículo http://purl.org/coar/resource_type/c_6501 Foods 13(6): 850 (2024) CEX2021-001189-S http://hdl.handle.net/10261/352787 10.3390/foods13060850 2304-8158 http://dx.doi.org/10.13039/501100005362 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105509RJ-I00/ES/ESTRATEGIA PARA PREVENIR LA CONTAMINACION DE NOROVIRUS EN LA CADENA ALIMENTARIA/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-S Publisher's version https://doi.org/10.3390/foods13060850 Sí open application/pdf Multidisciplinary Digital Publishing Institute
institution	IATA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-iata-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IATA España
language	English
topic	Norovirus Vibrio Bivalve mollusks Inactivation Food safety Bacteriophages Food processing technology Water quality Contamination Norovirus Vibrio Bivalve mollusks Inactivation Food safety Bacteriophages Food processing technology Water quality Contamination
spellingShingle	Norovirus Vibrio Bivalve mollusks Inactivation Food safety Bacteriophages Food processing technology Water quality Contamination Norovirus Vibrio Bivalve mollusks Inactivation Food safety Bacteriophages Food processing technology Water quality Contamination Pandiscia, Annamaria Lorusso, Patrizio Manfredi, Alessio Sánchez Moragas, Gloria Terio, Valentina Randazzo, Walter Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
description	© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
author2	Ministerio de Ciencia, Innovación y Universidades (España)
author_facet	Ministerio de Ciencia, Innovación y Universidades (España) Pandiscia, Annamaria Lorusso, Patrizio Manfredi, Alessio Sánchez Moragas, Gloria Terio, Valentina Randazzo, Walter
format	artículo
topic_facet	Norovirus Vibrio Bivalve mollusks Inactivation Food safety Bacteriophages Food processing technology Water quality Contamination
author	Pandiscia, Annamaria Lorusso, Patrizio Manfredi, Alessio Sánchez Moragas, Gloria Terio, Valentina Randazzo, Walter
author_sort	Pandiscia, Annamaria
title	Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
title_short	Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
title_full	Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
title_fullStr	Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
title_full_unstemmed	Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
title_sort	leveraging plasma-activated seawater for the control of human norovirus and bacterial pathogens in shellfish depuration
publisher	Multidisciplinary Digital Publishing Institute
publishDate	2024-03-02
url	http://hdl.handle.net/10261/352787 http://dx.doi.org/10.13039/501100005362 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004837
work_keys_str_mv	AT pandisciaannamaria leveragingplasmaactivatedseawaterforthecontrolofhumannorovirusandbacterialpathogensinshellfishdepuration AT lorussopatrizio leveragingplasmaactivatedseawaterforthecontrolofhumannorovirusandbacterialpathogensinshellfishdepuration AT manfredialessio leveragingplasmaactivatedseawaterforthecontrolofhumannorovirusandbacterialpathogensinshellfishdepuration AT sanchezmoragasgloria leveragingplasmaactivatedseawaterforthecontrolofhumannorovirusandbacterialpathogensinshellfishdepuration AT teriovalentina leveragingplasmaactivatedseawaterforthecontrolofhumannorovirusandbacterialpathogensinshellfishdepuration AT randazzowalter leveragingplasmaactivatedseawaterforthecontrolofhumannorovirusandbacterialpathogensinshellfishdepuration
_version_	1802820558186872832

Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration

Similar Items

Resource Map