Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet
This study explored the effects of Chenopodium quinoa's ingredients on the major lipids' hepatic profile and the functional selective differentiation of monocyte-derived macrophages and innate lymphoid cells in mice on a high-fat diet. Six-week-old Rag2-/- and Rag2-/-Il2-/- mice received (12 days) a low-molecular-weight protein fraction (LWPF) or the lipid fraction (qLF) obtained from the cold pressing of C. quinoa's germen. At the end of the experiment, mouse serum and liver tissue were collected. The differences in triglycerides, phospholipids, and the major lipids profile were analyzed. Infiltrated monocyte-derived macrophages and innate lymphoid cells (ILCs) and the expression of liver metabolic stress-related mRNA were measured. In the Rag2-/- mice, feeding them LWPF appeared to improve, to a larger extent, their hepatic capacity to utilize fatty acids in comparison to the qLF by preventing the overwhelming of triglycerides (TGs), despite both reducing the hepatic lipid accumulation. An analysis of the hepatic major lipids profile revealed significant increased variations in the PUFAs and phospholipid composition in the Rag2-/- mice fed with the LWPF or LF. The Rag2-/-Il2-/- mice, lacking innate and adaptive lymphocytes, seemed resistant to mobilizing hepatic TGs and unresponsive to lipid accumulation when fed with the LF. Notably, only the Rag2-/- mice fed with the LWPF showed an increased proportion of hepatic CD68+F4/80+ cells population, with a better controlled expression of the innate immune 'Toll-like' receptor (TLR)-4. These changes were associated with an oriented expansion of pluripotential CD117+ cells towards ILC2s (CD117+KLRG1+). Thus, C. quinoa's ingredients resulted in being advantageous for improving the mechanisms for controlling the hepatic lipotoxicity derived from a high-fat diet, promoting liver macrophage and ILCs expansion to a selective functional differentiation for the control of HFD-driven immune and metabolic disturbances.
Main Authors: | , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2023-09-04
|
Subjects: | High-fat-diet-induced obesity, Innate lymphoid cells, Lipid homeostasis, Macrophage, Quinoa, |
Online Access: | http://hdl.handle.net/10261/337596 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85170269486 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-iata-es-10261-337596 |
---|---|
record_format |
koha |
spelling |
dig-iata-es-10261-3375962024-05-15T20:37:34Z Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet Garcia Tejedor, Aurora Haros, Monika Laparra Llopis, José Moisés Agencia Estatal de Investigación (España) Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo 0000-0002-9790-0659 0000-0001-7904-0109 0000-0002-9378-2552 High-fat-diet-induced obesity Innate lymphoid cells Lipid homeostasis Macrophage Quinoa This study explored the effects of Chenopodium quinoa's ingredients on the major lipids' hepatic profile and the functional selective differentiation of monocyte-derived macrophages and innate lymphoid cells in mice on a high-fat diet. Six-week-old Rag2-/- and Rag2-/-Il2-/- mice received (12 days) a low-molecular-weight protein fraction (LWPF) or the lipid fraction (qLF) obtained from the cold pressing of C. quinoa's germen. At the end of the experiment, mouse serum and liver tissue were collected. The differences in triglycerides, phospholipids, and the major lipids profile were analyzed. Infiltrated monocyte-derived macrophages and innate lymphoid cells (ILCs) and the expression of liver metabolic stress-related mRNA were measured. In the Rag2-/- mice, feeding them LWPF appeared to improve, to a larger extent, their hepatic capacity to utilize fatty acids in comparison to the qLF by preventing the overwhelming of triglycerides (TGs), despite both reducing the hepatic lipid accumulation. An analysis of the hepatic major lipids profile revealed significant increased variations in the PUFAs and phospholipid composition in the Rag2-/- mice fed with the LWPF or LF. The Rag2-/-Il2-/- mice, lacking innate and adaptive lymphocytes, seemed resistant to mobilizing hepatic TGs and unresponsive to lipid accumulation when fed with the LF. Notably, only the Rag2-/- mice fed with the LWPF showed an increased proportion of hepatic CD68+F4/80+ cells population, with a better controlled expression of the innate immune 'Toll-like' receptor (TLR)-4. These changes were associated with an oriented expansion of pluripotential CD117+ cells towards ILC2s (CD117+KLRG1+). Thus, C. quinoa's ingredients resulted in being advantageous for improving the mechanisms for controlling the hepatic lipotoxicity derived from a high-fat diet, promoting liver macrophage and ILCs expansion to a selective functional differentiation for the control of HFD-driven immune and metabolic disturbances. This work was financially supported by Grant PID2019-107650RB-C22 funded by MCIN/AEI/10.13039/501100011033 and CYTED [La ValSe-Food, 119RT0S67]. Peer reviewed 2023-10-23T12:02:18Z 2023-10-23T12:02:18Z 2023-09-04 artículo http://purl.org/coar/resource_type/c_6501 Foods 12 (17): 3321 (2023) http://hdl.handle.net/10261/337596 10.3390/foods12173321 2304-8158 http://dx.doi.org/10.13039/501100011033 37685253 2-s2.0-85170269486 https://api.elsevier.com/content/abstract/scopus_id/85170269486 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107650RB-C22/ES/COMPRENSION DE LA INTERACCION DINAMICA DE LAS FORMULACIONES ALIMENTARIAS MEJORADAS CON BENEFICIOS INMUNONUTRICIONALES EN LA PREVENCION Y APARICION DE DISFUNCION HEPATICA/ Foods (Basel, Switzerland) Publisher's version https://doi.org/10.3390/foods12173321 Sí open Multidisciplinary Digital Publishing Institute |
institution |
IATA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-iata-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IATA España |
language |
English |
topic |
High-fat-diet-induced obesity Innate lymphoid cells Lipid homeostasis Macrophage Quinoa High-fat-diet-induced obesity Innate lymphoid cells Lipid homeostasis Macrophage Quinoa |
spellingShingle |
High-fat-diet-induced obesity Innate lymphoid cells Lipid homeostasis Macrophage Quinoa High-fat-diet-induced obesity Innate lymphoid cells Lipid homeostasis Macrophage Quinoa Garcia Tejedor, Aurora Haros, Monika Laparra Llopis, José Moisés Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet |
description |
This study explored the effects of Chenopodium quinoa's ingredients on the major lipids' hepatic profile and the functional selective differentiation of monocyte-derived macrophages and innate lymphoid cells in mice on a high-fat diet. Six-week-old Rag2-/- and Rag2-/-Il2-/- mice received (12 days) a low-molecular-weight protein fraction (LWPF) or the lipid fraction (qLF) obtained from the cold pressing of C. quinoa's germen. At the end of the experiment, mouse serum and liver tissue were collected. The differences in triglycerides, phospholipids, and the major lipids profile were analyzed. Infiltrated monocyte-derived macrophages and innate lymphoid cells (ILCs) and the expression of liver metabolic stress-related mRNA were measured. In the Rag2-/- mice, feeding them LWPF appeared to improve, to a larger extent, their hepatic capacity to utilize fatty acids in comparison to the qLF by preventing the overwhelming of triglycerides (TGs), despite both reducing the hepatic lipid accumulation. An analysis of the hepatic major lipids profile revealed significant increased variations in the PUFAs and phospholipid composition in the Rag2-/- mice fed with the LWPF or LF. The Rag2-/-Il2-/- mice, lacking innate and adaptive lymphocytes, seemed resistant to mobilizing hepatic TGs and unresponsive to lipid accumulation when fed with the LF. Notably, only the Rag2-/- mice fed with the LWPF showed an increased proportion of hepatic CD68+F4/80+ cells population, with a better controlled expression of the innate immune 'Toll-like' receptor (TLR)-4. These changes were associated with an oriented expansion of pluripotential CD117+ cells towards ILC2s (CD117+KLRG1+). Thus, C. quinoa's ingredients resulted in being advantageous for improving the mechanisms for controlling the hepatic lipotoxicity derived from a high-fat diet, promoting liver macrophage and ILCs expansion to a selective functional differentiation for the control of HFD-driven immune and metabolic disturbances. |
author2 |
Agencia Estatal de Investigación (España) |
author_facet |
Agencia Estatal de Investigación (España) Garcia Tejedor, Aurora Haros, Monika Laparra Llopis, José Moisés |
format |
artículo |
topic_facet |
High-fat-diet-induced obesity Innate lymphoid cells Lipid homeostasis Macrophage Quinoa |
author |
Garcia Tejedor, Aurora Haros, Monika Laparra Llopis, José Moisés |
author_sort |
Garcia Tejedor, Aurora |
title |
Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet |
title_short |
Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet |
title_full |
Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet |
title_fullStr |
Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet |
title_full_unstemmed |
Chenopodium quinoa's Ingredients Improve Control of the Hepatic Lipid Disturbances Derived from a High-Fat Diet |
title_sort |
chenopodium quinoa's ingredients improve control of the hepatic lipid disturbances derived from a high-fat diet |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023-09-04 |
url |
http://hdl.handle.net/10261/337596 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85170269486 |
work_keys_str_mv |
AT garciatejedoraurora chenopodiumquinoasingredientsimprovecontrolofthehepaticlipiddisturbancesderivedfromahighfatdiet AT harosmonika chenopodiumquinoasingredientsimprovecontrolofthehepaticlipiddisturbancesderivedfromahighfatdiet AT laparrallopisjosemoises chenopodiumquinoasingredientsimprovecontrolofthehepaticlipiddisturbancesderivedfromahighfatdiet |
_version_ |
1802820543723864064 |