In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats
Novel peptides with antihypertensive effects in SHR rats have previously been identified in lactoferrin (LF) hydrolysates. To investigate their in vivo antihypertensive mechanism, we have assessed the blood pressure lowering effects of two of these LF-derived peptides (RPYL and DPYKLRP) in Wistar rats subjected to either angiotensin I- or angiotensin II-induced hypertension. Blood pressure was measured by the tail-cuff method, hypertension was induced by subcutaneous infusion of angiotensins, and then captopril, valsartan or LF-derived peptides orally administered. Angiotensin I- and angiotensin II-induced hypertension were reversed by captopril and valsartan, respectively. RPYL and DPYKLRP reversed angiotensin I-induced hypertension, while DPYKLRP but not RPYL produced a modest reversion of angiotensin II-elicited hypertension. Neither RPYL nor DPYKLRP modified normotension. Thus, in vivo ACE inhibition is involved in the antihypertensive effects of LF-derived peptides like RPYL and DPYKLRP, while inhibition of AT1 receptor-mediated vasoconstriction plays a less relevant role.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2015-04-11
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Subjects: | Antihypertensive peptides, Lactoferrin-derived peptides, Angiotensin-induced hypertension, Wistar rat, In vivo ACE inhibition, Renin angiotensin system, |
Online Access: | http://hdl.handle.net/10261/148431 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004587 |
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dig-iata-es-10261-1484312017-07-05T11:48:27Z In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats García Tejedor, Aurora Castelló-Ruiz, María Gimeno Alcañiz, José Vicente Manzanares, Paloma Salom, Juan B. Ministerio de Educación y Ciencia (España) European Commission Instituto de Salud Carlos III Antihypertensive peptides Lactoferrin-derived peptides Angiotensin-induced hypertension Wistar rat In vivo ACE inhibition Renin angiotensin system Novel peptides with antihypertensive effects in SHR rats have previously been identified in lactoferrin (LF) hydrolysates. To investigate their in vivo antihypertensive mechanism, we have assessed the blood pressure lowering effects of two of these LF-derived peptides (RPYL and DPYKLRP) in Wistar rats subjected to either angiotensin I- or angiotensin II-induced hypertension. Blood pressure was measured by the tail-cuff method, hypertension was induced by subcutaneous infusion of angiotensins, and then captopril, valsartan or LF-derived peptides orally administered. Angiotensin I- and angiotensin II-induced hypertension were reversed by captopril and valsartan, respectively. RPYL and DPYKLRP reversed angiotensin I-induced hypertension, while DPYKLRP but not RPYL produced a modest reversion of angiotensin II-elicited hypertension. Neither RPYL nor DPYKLRP modified normotension. Thus, in vivo ACE inhibition is involved in the antihypertensive effects of LF-derived peptides like RPYL and DPYKLRP, while inhibition of AT1 receptor-mediated vasoconstriction plays a less relevant role. This work was supported by grant AGL2010-21009 from ‘Ministerio de Educación y Ciencia – FEDER’, Consolider Ingenio 2010, Fun-C-Food, CSD2007-00063 and RETICS INVICTUS RD12/0014/0004 from ‘Instituto de Salud Carlos III’. A. García-Tejedor is recipient of a predoctoral fellowship from ‘Ministerio de Educación y Ciencia’ (BES-2011-044424). Peer reviewed 2017-04-18T10:40:39Z 2017-04-18T10:40:39Z 2015-04-11 artículo http://purl.org/coar/resource_type/c_6501 Journal of Functional Foods 15: 294-300 (2015) 1756-4646 http://hdl.handle.net/10261/148431 10.1016/j.jff.2015.03.039 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004587 en Postprint http://dx.doi.org/10.1016/j.jff.2015.03.039 Sí open Elsevier |
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Antihypertensive peptides Lactoferrin-derived peptides Angiotensin-induced hypertension Wistar rat In vivo ACE inhibition Renin angiotensin system Antihypertensive peptides Lactoferrin-derived peptides Angiotensin-induced hypertension Wistar rat In vivo ACE inhibition Renin angiotensin system |
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Antihypertensive peptides Lactoferrin-derived peptides Angiotensin-induced hypertension Wistar rat In vivo ACE inhibition Renin angiotensin system Antihypertensive peptides Lactoferrin-derived peptides Angiotensin-induced hypertension Wistar rat In vivo ACE inhibition Renin angiotensin system García Tejedor, Aurora Castelló-Ruiz, María Gimeno Alcañiz, José Vicente Manzanares, Paloma Salom, Juan B. In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats |
description |
Novel peptides with antihypertensive effects in SHR rats have previously been identified in lactoferrin (LF) hydrolysates. To investigate their in vivo antihypertensive mechanism, we have assessed the blood pressure lowering effects of two of these LF-derived peptides (RPYL and DPYKLRP) in Wistar rats subjected to either angiotensin I- or angiotensin II-induced hypertension. Blood pressure was measured by the tail-cuff method, hypertension was induced by subcutaneous infusion of angiotensins, and then captopril, valsartan or LF-derived peptides orally administered. Angiotensin I- and angiotensin II-induced hypertension were reversed by captopril and valsartan, respectively. RPYL and DPYKLRP reversed angiotensin I-induced hypertension, while DPYKLRP but not RPYL produced a modest reversion of angiotensin II-elicited hypertension. Neither RPYL nor DPYKLRP modified normotension. Thus, in vivo ACE inhibition is involved in the antihypertensive effects of LF-derived peptides like RPYL and DPYKLRP, while inhibition of AT1 receptor-mediated vasoconstriction plays a less relevant role. |
author2 |
Ministerio de Educación y Ciencia (España) |
author_facet |
Ministerio de Educación y Ciencia (España) García Tejedor, Aurora Castelló-Ruiz, María Gimeno Alcañiz, José Vicente Manzanares, Paloma Salom, Juan B. |
format |
artículo |
topic_facet |
Antihypertensive peptides Lactoferrin-derived peptides Angiotensin-induced hypertension Wistar rat In vivo ACE inhibition Renin angiotensin system |
author |
García Tejedor, Aurora Castelló-Ruiz, María Gimeno Alcañiz, José Vicente Manzanares, Paloma Salom, Juan B. |
author_sort |
García Tejedor, Aurora |
title |
In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats |
title_short |
In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats |
title_full |
In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats |
title_fullStr |
In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats |
title_full_unstemmed |
In vivo antihypertensive mechanism of lactoferrin-derived peptides: Reversion of angiotensin I- and angiotensin II-induced hypertension in Wistar rats |
title_sort |
in vivo antihypertensive mechanism of lactoferrin-derived peptides: reversion of angiotensin i- and angiotensin ii-induced hypertension in wistar rats |
publisher |
Elsevier |
publishDate |
2015-04-11 |
url |
http://hdl.handle.net/10261/148431 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004587 |
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