Genomic profiling of plastid DNA variation in the Mediterranean olive tree
[Background] Characterisation of plastid genome (or cpDNA) polymorphisms is commonly used for phylogeographic, population genetic and forensic analyses in plants, but detecting cpDNA variation is sometimes challenging, limiting the applications of such an approach. In the present study, we screened cpDNA polymorphism in the olive tree (Olea europaea L.) by sequencing the complete plastid genome of trees with a distinct cpDNA lineage. Our objective was to develop new markers for a rapid genomic profiling (by Multiplex PCRs) of cpDNA haplotypes in the Mediterranean olive tree.
Main Authors: | , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
BioMed Central
2011-05-10
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Subjects: | Olive Tree, Plastid Genome, Microsatellite Motif, cpDNA haplotypes, cpDNA Variation, |
Online Access: | http://hdl.handle.net/10261/39023 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100000275 http://dx.doi.org/10.13039/501100000704 http://dx.doi.org/10.13039/501100000781 http://dx.doi.org/10.13039/501100000270 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011011 |
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Summary: | [Background] Characterisation of plastid genome (or cpDNA) polymorphisms is commonly used for phylogeographic, population genetic and forensic analyses in plants, but detecting cpDNA variation is sometimes challenging, limiting the applications of such an approach. In the present study, we screened cpDNA polymorphism in the olive tree (Olea europaea L.) by sequencing the complete plastid genome of trees with a distinct cpDNA lineage. Our objective was to develop new markers for a rapid genomic profiling (by Multiplex PCRs) of cpDNA haplotypes in the Mediterranean olive tree. |
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