Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm
Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the major constraints of pea worldwide. Its control is difficult and is mainly based on the use of resistant cultivars. This study aimed to identify and characterize resistance mechanisms interfering with Fop spore germination, as an additional pre-penetration resistance mechanism little explored so far. For this, root exudates were collected from 12 pea accessions with differential responses to the disease, from resistant to susceptible, and their effects on Fop germination and growth were determined. While root exudates from most accessions stimulated Fop germination, the root exudates of three accessions, JI 1412, JI 2480 and P42, did not stimulate, or even inhibited, Fop germination. Although some additional compounds might be involved, the analysis showed that the most active metabolite was the pea phytoalexin pisatin. Pisatin was identified in the active fraction of pea root exudate extracts and its amount in the root exudates was negatively correlated with the extent of Fop germination. This suggests an important role of pisatin in the constitutive defence of pea against F. oxysporum.
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Format: | artículo biblioteca |
Language: | English |
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John Wiley & Sons
2018-06
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Subjects: | Root exudates, Constitutive defence reaction, Fusarium wilt, Pisatin, Pisum sativum, Resistance mechanisms, |
Online Access: | http://hdl.handle.net/10261/351794 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85041124200 |
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dig-ias-es-10261-3517942024-05-14T21:19:07Z Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm Bani, Moustapha Cimmino, Alessio Evidente, Antonio Rubiales, Diego Rispail, Nicolas European Commission Ministerio de Economía y Competitividad (España) Root exudates Constitutive defence reaction Fusarium wilt Pisatin Pisum sativum Resistance mechanisms Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the major constraints of pea worldwide. Its control is difficult and is mainly based on the use of resistant cultivars. This study aimed to identify and characterize resistance mechanisms interfering with Fop spore germination, as an additional pre-penetration resistance mechanism little explored so far. For this, root exudates were collected from 12 pea accessions with differential responses to the disease, from resistant to susceptible, and their effects on Fop germination and growth were determined. While root exudates from most accessions stimulated Fop germination, the root exudates of three accessions, JI 1412, JI 2480 and P42, did not stimulate, or even inhibited, Fop germination. Although some additional compounds might be involved, the analysis showed that the most active metabolite was the pea phytoalexin pisatin. Pisatin was identified in the active fraction of pea root exudate extracts and its amount in the root exudates was negatively correlated with the extent of Fop germination. This suggests an important role of pisatin in the constitutive defence of pea against F. oxysporum. This work was supported by the European KBBE project LEGATO (FP7-KBBE2013.1.2-02-613551), and the Spanish national project AGL2014-52871-R from the Spanish Ministry of Economy and Competitiveness (MINECO) co-financed by the European fund for regional development (FEDER). N.R. is the holder of a Ramón y Cajal postdoctoral position from the MINECO. Peer reviewed 2024-03-25T15:33:23Z 2024-03-25T15:33:23Z 2018-06 artículo http://purl.org/coar/resource_type/c_6501 Plant Pathology 67(5): 1046-1054 (2018) 0032-0862 http://hdl.handle.net/10261/351794 10.1111/ppa.12813 1365-3059 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 2-s2.0-85041124200 https://api.elsevier.com/content/abstract/scopus_id/85041124200 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/613551 info:eu-repo/grantAgreement/MINECO//AGL2014-52871-R/ES/MEJORA GENETICA DEL GUISANTE POR RESISTENCIA A ENFERMEDADES/ Postprint https://doi.org/10.1111/ppa.12813 Sí open application/pdf John Wiley & Sons |
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Root exudates Constitutive defence reaction Fusarium wilt Pisatin Pisum sativum Resistance mechanisms Root exudates Constitutive defence reaction Fusarium wilt Pisatin Pisum sativum Resistance mechanisms |
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Root exudates Constitutive defence reaction Fusarium wilt Pisatin Pisum sativum Resistance mechanisms Root exudates Constitutive defence reaction Fusarium wilt Pisatin Pisum sativum Resistance mechanisms Bani, Moustapha Cimmino, Alessio Evidente, Antonio Rubiales, Diego Rispail, Nicolas Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm |
description |
Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the major constraints of pea worldwide. Its control is difficult and is mainly based on the use of resistant cultivars. This study aimed to identify and characterize resistance mechanisms interfering with Fop spore germination, as an additional pre-penetration resistance mechanism little explored so far. For this, root exudates were collected from 12 pea accessions with differential responses to the disease, from resistant to susceptible, and their effects on Fop germination and growth were determined. While root exudates from most accessions stimulated Fop germination, the root exudates of three accessions, JI 1412, JI 2480 and P42, did not stimulate, or even inhibited, Fop germination. Although some additional compounds might be involved, the analysis showed that the most active metabolite was the pea phytoalexin pisatin. Pisatin was identified in the active fraction of pea root exudate extracts and its amount in the root exudates was negatively correlated with the extent of Fop germination. This suggests an important role of pisatin in the constitutive defence of pea against F. oxysporum. |
author2 |
European Commission |
author_facet |
European Commission Bani, Moustapha Cimmino, Alessio Evidente, Antonio Rubiales, Diego Rispail, Nicolas |
format |
artículo |
topic_facet |
Root exudates Constitutive defence reaction Fusarium wilt Pisatin Pisum sativum Resistance mechanisms |
author |
Bani, Moustapha Cimmino, Alessio Evidente, Antonio Rubiales, Diego Rispail, Nicolas |
author_sort |
Bani, Moustapha |
title |
Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm |
title_short |
Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm |
title_full |
Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm |
title_fullStr |
Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm |
title_full_unstemmed |
Pisatin involvement in the variation of inhibition of Fusarium oxysporum f. sp. pisi spore germination by root exudates of Pisum spp. germplasm |
title_sort |
pisatin involvement in the variation of inhibition of fusarium oxysporum f. sp. pisi spore germination by root exudates of pisum spp. germplasm |
publisher |
John Wiley & Sons |
publishDate |
2018-06 |
url |
http://hdl.handle.net/10261/351794 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85041124200 |
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