High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci

High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci

© 2020 by the authors.

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Bibliographic Details
Main Authors: Mérida-García, Rosa, Gálvez, Sergio, Paux, Etienne, Dorado, Gabriel, Pascual, Laura, Giraldo, Patricia, Hernández Molina, Pilar
Other Authors: Ministerio de Ciencia, Innovación y Universidades (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020-09-01
Subjects:High resolution melting, ISBP markers, Drought, Candidate genes, QTL, MQTL, Wheat variability,
Online Access:http://hdl.handle.net/10261/220275
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100011011
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spelling dig-ias-es-10261-2202752021-01-15T11:26:01Z High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci Mérida-García, Rosa Gálvez, Sergio Paux, Etienne Dorado, Gabriel Pascual, Laura Giraldo, Patricia Hernández Molina, Pilar Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Junta de Andalucía High resolution melting ISBP markers Drought Candidate genes QTL MQTL Wheat variability © 2020 by the authors. The practical use of molecular markers is facilitated by cost-effective detection techniques. In this work, wheat insertion site-based polymorphisms (ISBP) markers were set up for genotyping using high-resolution melting analysis (HRM). Polymorphic HRM-ISBP assays were developed for wheat chromosomes 4A and 3B and used for wheat variability assessment. The marker sequences were mapped against the wheat genome reference sequence, targeting interesting genes. Those genes were located within or in proximity to previously described quantitative trait loci (QTL) or meta-quantitative trait loci (MQTL) for drought and heat stress tolerance, and also yield and yield related traits. Eighteen of the markers used tagged drought related genes and, interestingly, eight of the genes were differentially expressed under different abiotic stress conditions. These results confirmed HRM as a cost-effective and efficient tool for wheat breeding programs. This research was funded by project P18-RT-992 from Junta de Andalucía (Andalusian Regional Government), Spain (Co-funded by FEDER), and by the Spanish Ministry of Science and Innovation project PID2019-109089RB-C32. Peer reviewed 2020-09-25T13:32:38Z 2020-09-25T13:32:38Z 2020-09-01 2020-09-25T13:32:38Z artículo http://purl.org/coar/resource_type/c_6501 Agronomy 10(9): 1294 (2020) http://hdl.handle.net/10261/220275 10.3390/agronomy10091294 2073-4395 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100011011 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109089RB-C32 PID2019-109089RB-C32/AEI/10.13039/501100011033 Publisher’s version https://doi.org/10.3390/agronomy10091294 Sí open Multidisciplinary Digital Publishing Institute
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
topic High resolution melting
ISBP markers
Drought
Candidate genes
QTL
MQTL
Wheat variability
High resolution melting
ISBP markers
Drought
Candidate genes
QTL
MQTL
Wheat variability
spellingShingle High resolution melting
ISBP markers
Drought
Candidate genes
QTL
MQTL
Wheat variability
High resolution melting
ISBP markers
Drought
Candidate genes
QTL
MQTL
Wheat variability
Mérida-García, Rosa
Gálvez, Sergio
Paux, Etienne
Dorado, Gabriel
Pascual, Laura
Giraldo, Patricia
Hernández Molina, Pilar
High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
description © 2020 by the authors.
author2 Ministerio de Ciencia, Innovación y Universidades (España)
author_facet Ministerio de Ciencia, Innovación y Universidades (España)
Mérida-García, Rosa
Gálvez, Sergio
Paux, Etienne
Dorado, Gabriel
Pascual, Laura
Giraldo, Patricia
Hernández Molina, Pilar
format artículo
topic_facet High resolution melting
ISBP markers
Drought
Candidate genes
QTL
MQTL
Wheat variability
author Mérida-García, Rosa
Gálvez, Sergio
Paux, Etienne
Dorado, Gabriel
Pascual, Laura
Giraldo, Patricia
Hernández Molina, Pilar
author_sort Mérida-García, Rosa
title High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
title_short High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
title_full High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
title_fullStr High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
title_full_unstemmed High Resolution Melting and Insertion Site-Based Polymorphism Markers for Wheat Variability Analysis and Candidate Genes Selection at Drought and Heat MQTL Loci
title_sort high resolution melting and insertion site-based polymorphism markers for wheat variability analysis and candidate genes selection at drought and heat mqtl loci
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020-09-01
url http://hdl.handle.net/10261/220275
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100011011
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