Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques
The potential for population genomics to elucidate invasion pathways of a species is limited by taxonomic identification issues. The Oriental fruit fly pest, Bactrocera dorsalis (Hendel) belongs to a complex in which several sympatric species are attracted to the same lure used in trapping and are morphologically cryptic and/or reported to hybridize. In this study, we evaluated the taxonomic ambiguity between B. dorsalis and 2 major cryptic species, based on morphological expertise and 289 target specimens sampled across the whole distribution range. Specimens were then subjected to DNA sequence analyses of the COI mitochondrial barcode and the EIF3L nuclear marker to evaluate the potential for molecular identification, in particular for specimens for which morphological identification was inconclusive. To this aim, we produced reference datasets with DNA sequences from target specimens whose morphological identification was unambiguous, which we complemented with 56 new DNA sequences from closest relatives and 76 published and curated DNA sequences of different species in the complex. After the necessary morphological observation, about 3.5% of the target dataset and 47.6% of the specimens from Southeast Asian islands displayed ambiguous character states shared with B. carambolae and/or B. occipitalis. Critical interpretation of DNA sequence data solved morphological ambiguities only when combining both mitochondrial and nuclear markers. COI discriminated B. dorsalis from 5 species; EIF3L and ITS from another species. We recommend this procedure to ensure correct identification of B. dorsalis specimens in population genetics studies and surveillance programs.
Main Authors: | , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H10 - Ravageurs des plantes, identification, Bactrocera dorsalis, Tephritidae, marqueur génétique, taxonomie, Bactrocera, adn, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_36022, http://aims.fao.org/aos/agrovoc/c_3122, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_7631, http://aims.fao.org/aos/agrovoc/c_33628, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_165, http://aims.fao.org/aos/agrovoc/c_1229, |
Online Access: | http://agritrop.cirad.fr/606712/ http://agritrop.cirad.fr/606712/7/606712.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-606712 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-6067122024-02-28T14:29:12Z http://agritrop.cirad.fr/606712/ http://agritrop.cirad.fr/606712/ Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques. Charbonnel Emeline, Chapuis Marie-Pierre, Taddei Andrea, Schutze Mark K., Starkie Melissa L., Benoit Laure, Mouttet Raphëlle, Ouvrard David. 2023. Journal of Economic Entomology, 116 (6) : 2193-2200.https://doi.org/10.1093/jee/toad178 <https://doi.org/10.1093/jee/toad178> Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques Charbonnel, Emeline Chapuis, Marie-Pierre Taddei, Andrea Schutze, Mark K. Starkie, Melissa L. Benoit, Laure Mouttet, Raphëlle Ouvrard, David eng 2023 Journal of Economic Entomology H10 - Ravageurs des plantes identification Bactrocera dorsalis Tephritidae marqueur génétique taxonomie Bactrocera adn http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_36022 http://aims.fao.org/aos/agrovoc/c_3122 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_33628 http://aims.fao.org/aos/agrovoc/c_2347 Afrique Cameroun http://aims.fao.org/aos/agrovoc/c_165 http://aims.fao.org/aos/agrovoc/c_1229 The potential for population genomics to elucidate invasion pathways of a species is limited by taxonomic identification issues. The Oriental fruit fly pest, Bactrocera dorsalis (Hendel) belongs to a complex in which several sympatric species are attracted to the same lure used in trapping and are morphologically cryptic and/or reported to hybridize. In this study, we evaluated the taxonomic ambiguity between B. dorsalis and 2 major cryptic species, based on morphological expertise and 289 target specimens sampled across the whole distribution range. Specimens were then subjected to DNA sequence analyses of the COI mitochondrial barcode and the EIF3L nuclear marker to evaluate the potential for molecular identification, in particular for specimens for which morphological identification was inconclusive. To this aim, we produced reference datasets with DNA sequences from target specimens whose morphological identification was unambiguous, which we complemented with 56 new DNA sequences from closest relatives and 76 published and curated DNA sequences of different species in the complex. After the necessary morphological observation, about 3.5% of the target dataset and 47.6% of the specimens from Southeast Asian islands displayed ambiguous character states shared with B. carambolae and/or B. occipitalis. Critical interpretation of DNA sequence data solved morphological ambiguities only when combining both mitochondrial and nuclear markers. COI discriminated B. dorsalis from 5 species; EIF3L and ITS from another species. We recommend this procedure to ensure correct identification of B. dorsalis specimens in population genetics studies and surveillance programs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/606712/7/606712.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1093/jee/toad178 10.1093/jee/toad178 info:eu-repo/semantics/altIdentifier/doi/10.1093/jee/toad178 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/jee/toad178 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/?term=OR208544 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/?term=OR208544 info:eu-repo/grantAgreement/////(FRA) BACTRACK/ |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H10 - Ravageurs des plantes identification Bactrocera dorsalis Tephritidae marqueur génétique taxonomie Bactrocera adn http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_36022 http://aims.fao.org/aos/agrovoc/c_3122 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_33628 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_165 http://aims.fao.org/aos/agrovoc/c_1229 H10 - Ravageurs des plantes identification Bactrocera dorsalis Tephritidae marqueur génétique taxonomie Bactrocera adn http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_36022 http://aims.fao.org/aos/agrovoc/c_3122 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_33628 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_165 http://aims.fao.org/aos/agrovoc/c_1229 |
spellingShingle |
H10 - Ravageurs des plantes identification Bactrocera dorsalis Tephritidae marqueur génétique taxonomie Bactrocera adn http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_36022 http://aims.fao.org/aos/agrovoc/c_3122 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_33628 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_165 http://aims.fao.org/aos/agrovoc/c_1229 H10 - Ravageurs des plantes identification Bactrocera dorsalis Tephritidae marqueur génétique taxonomie Bactrocera adn http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_36022 http://aims.fao.org/aos/agrovoc/c_3122 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_33628 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_165 http://aims.fao.org/aos/agrovoc/c_1229 Charbonnel, Emeline Chapuis, Marie-Pierre Taddei, Andrea Schutze, Mark K. Starkie, Melissa L. Benoit, Laure Mouttet, Raphëlle Ouvrard, David Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques |
description |
The potential for population genomics to elucidate invasion pathways of a species is limited by taxonomic identification issues. The Oriental fruit fly pest, Bactrocera dorsalis (Hendel) belongs to a complex in which several sympatric species are attracted to the same lure used in trapping and are morphologically cryptic and/or reported to hybridize. In this study, we evaluated the taxonomic ambiguity between B. dorsalis and 2 major cryptic species, based on morphological expertise and 289 target specimens sampled across the whole distribution range. Specimens were then subjected to DNA sequence analyses of the COI mitochondrial barcode and the EIF3L nuclear marker to evaluate the potential for molecular identification, in particular for specimens for which morphological identification was inconclusive. To this aim, we produced reference datasets with DNA sequences from target specimens whose morphological identification was unambiguous, which we complemented with 56 new DNA sequences from closest relatives and 76 published and curated DNA sequences of different species in the complex. After the necessary morphological observation, about 3.5% of the target dataset and 47.6% of the specimens from Southeast Asian islands displayed ambiguous character states shared with B. carambolae and/or B. occipitalis. Critical interpretation of DNA sequence data solved morphological ambiguities only when combining both mitochondrial and nuclear markers. COI discriminated B. dorsalis from 5 species; EIF3L and ITS from another species. We recommend this procedure to ensure correct identification of B. dorsalis specimens in population genetics studies and surveillance programs. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes identification Bactrocera dorsalis Tephritidae marqueur génétique taxonomie Bactrocera adn http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_36022 http://aims.fao.org/aos/agrovoc/c_3122 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_33628 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_165 http://aims.fao.org/aos/agrovoc/c_1229 |
author |
Charbonnel, Emeline Chapuis, Marie-Pierre Taddei, Andrea Schutze, Mark K. Starkie, Melissa L. Benoit, Laure Mouttet, Raphëlle Ouvrard, David |
author_facet |
Charbonnel, Emeline Chapuis, Marie-Pierre Taddei, Andrea Schutze, Mark K. Starkie, Melissa L. Benoit, Laure Mouttet, Raphëlle Ouvrard, David |
author_sort |
Charbonnel, Emeline |
title |
Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques |
title_short |
Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques |
title_full |
Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques |
title_fullStr |
Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques |
title_full_unstemmed |
Evaluation of identification methods for cryptic Bactrocera dorsalis (Diptera: Tephritidae) specimens: Combining morphological and molecular techniques |
title_sort |
evaluation of identification methods for cryptic bactrocera dorsalis (diptera: tephritidae) specimens: combining morphological and molecular techniques |
url |
http://agritrop.cirad.fr/606712/ http://agritrop.cirad.fr/606712/7/606712.pdf |
work_keys_str_mv |
AT charbonnelemeline evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT chapuismariepierre evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT taddeiandrea evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT schutzemarkk evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT starkiemelissal evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT benoitlaure evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT mouttetraphelle evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques AT ouvrarddavid evaluationofidentificationmethodsforcrypticbactroceradorsalisdipteratephritidaespecimenscombiningmorphologicalandmoleculartechniques |
_version_ |
1792500650090692608 |