Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals
Many emerging infectious diseases originate from wild animals, so there is a profound need for surveillance and monitoring of their pathogens. However, the practical difficulty of sample acquisition from wild animals tends to limit the feasibility and effectiveness of such surveys. Xenosurveillance, using blood-feeding invertebrates to obtain tissue samples from wild animals and then detect their pathogens, is a promising method to do so. Here, we describe the use of tsetse fly blood meals to determine (directly through molecular diagnostic and indirectly through serology), the diversity of circulating blood-borne pathogens (including bacteria, viruses and protozoa) in a natural mammalian community of Tanzania. Molecular analyses of captured tsetse flies (182 pools of flies totalizing 1728 flies) revealed that the blood meals obtained came from 18 different vertebrate species including 16 non-human mammals, representing approximately 25% of the large mammal species present in the study area. Molecular diagnostic demonstrated the presence of different protozoa parasites and bacteria of medical and/or veterinary interest. None of the six virus species searched for by molecular methods were detected but an ELISA test detected antibodies against African swine fever virus among warthogs, indicating that the virus had been circulating in the area. Sampling of blood-feeding insects represents an efficient and practical approach to tracking a diversity of pathogens from multiple mammalian species, directly through molecular diagnostic or indirectly through serology, which could readily expand and enhance our understanding of the ecology and evolution of infectious agents and their interactions with their hosts in wild animal communities.
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Biblioteca del CIRAD Francia |
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transmission des maladies animal sauvage relation hôte pathogène agent pathogène écologie animale surveillance épidémiologique viande de gibier maladie infectieuse maladie des animaux zoonose virus peste porcine africaine identification Glossina sérologie http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_5630 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_3183 http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_11174 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_7608 transmission des maladies animal sauvage relation hôte pathogène agent pathogène écologie animale surveillance épidémiologique viande de gibier maladie infectieuse maladie des animaux zoonose virus peste porcine africaine identification Glossina sérologie http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_5630 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_3183 http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_11174 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_7608 |
spellingShingle |
transmission des maladies animal sauvage relation hôte pathogène agent pathogène écologie animale surveillance épidémiologique viande de gibier maladie infectieuse maladie des animaux zoonose virus peste porcine africaine identification Glossina sérologie http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_5630 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_3183 http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_11174 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_7608 transmission des maladies animal sauvage relation hôte pathogène agent pathogène écologie animale surveillance épidémiologique viande de gibier maladie infectieuse maladie des animaux zoonose virus peste porcine africaine identification Glossina sérologie http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_5630 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_3183 http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_11174 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_7608 Mwakasungula, Solomon Rougeron, Virginie Arnathau, Céline Boundenga, Larson Miguel, Eve Boissiere, Anne Jiolle, Davy Durand, Patrick Msigwa, Alphonce Mswata, Sarah Olotu, Ally Sterkers, Yvon Roche, Benjamin Killeen, Gerard Cerqueira, Frédérique Bitome-Essono, Paul Yannick Bretagnolle, François Masanja, Honorati Paupy, Christophe Sumaye, Robert Prugnolle, Franck Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
description |
Many emerging infectious diseases originate from wild animals, so there is a profound need for surveillance and monitoring of their pathogens. However, the practical difficulty of sample acquisition from wild animals tends to limit the feasibility and effectiveness of such surveys. Xenosurveillance, using blood-feeding invertebrates to obtain tissue samples from wild animals and then detect their pathogens, is a promising method to do so. Here, we describe the use of tsetse fly blood meals to determine (directly through molecular diagnostic and indirectly through serology), the diversity of circulating blood-borne pathogens (including bacteria, viruses and protozoa) in a natural mammalian community of Tanzania. Molecular analyses of captured tsetse flies (182 pools of flies totalizing 1728 flies) revealed that the blood meals obtained came from 18 different vertebrate species including 16 non-human mammals, representing approximately 25% of the large mammal species present in the study area. Molecular diagnostic demonstrated the presence of different protozoa parasites and bacteria of medical and/or veterinary interest. None of the six virus species searched for by molecular methods were detected but an ELISA test detected antibodies against African swine fever virus among warthogs, indicating that the virus had been circulating in the area. Sampling of blood-feeding insects represents an efficient and practical approach to tracking a diversity of pathogens from multiple mammalian species, directly through molecular diagnostic or indirectly through serology, which could readily expand and enhance our understanding of the ecology and evolution of infectious agents and their interactions with their hosts in wild animal communities. |
format |
article |
topic_facet |
transmission des maladies animal sauvage relation hôte pathogène agent pathogène écologie animale surveillance épidémiologique viande de gibier maladie infectieuse maladie des animaux zoonose virus peste porcine africaine identification Glossina sérologie http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_5630 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_3183 http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_11174 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_7608 |
author |
Mwakasungula, Solomon Rougeron, Virginie Arnathau, Céline Boundenga, Larson Miguel, Eve Boissiere, Anne Jiolle, Davy Durand, Patrick Msigwa, Alphonce Mswata, Sarah Olotu, Ally Sterkers, Yvon Roche, Benjamin Killeen, Gerard Cerqueira, Frédérique Bitome-Essono, Paul Yannick Bretagnolle, François Masanja, Honorati Paupy, Christophe Sumaye, Robert Prugnolle, Franck |
author_facet |
Mwakasungula, Solomon Rougeron, Virginie Arnathau, Céline Boundenga, Larson Miguel, Eve Boissiere, Anne Jiolle, Davy Durand, Patrick Msigwa, Alphonce Mswata, Sarah Olotu, Ally Sterkers, Yvon Roche, Benjamin Killeen, Gerard Cerqueira, Frédérique Bitome-Essono, Paul Yannick Bretagnolle, François Masanja, Honorati Paupy, Christophe Sumaye, Robert Prugnolle, Franck |
author_sort |
Mwakasungula, Solomon |
title |
Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
title_short |
Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
title_full |
Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
title_fullStr |
Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
title_full_unstemmed |
Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
title_sort |
using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals |
url |
http://agritrop.cirad.fr/605750/ http://agritrop.cirad.fr/605750/1/Mwankasungula%20et%20al%2C%202022.pdf |
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dig-cirad-fr-6057502024-08-27T15:21:46Z http://agritrop.cirad.fr/605750/ http://agritrop.cirad.fr/605750/ Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals. Mwakasungula Solomon, Rougeron Virginie, Arnathau Céline, Boundenga Larson, Miguel Eve, Boissiere Anne, Jiolle Davy, Durand Patrick, Msigwa Alphonce, Mswata Sarah, Olotu Ally, Sterkers Yvon, Roche Benjamin, Killeen Gerard, Cerqueira Frédérique, Bitome-Essono Paul Yannick, Bretagnolle François, Masanja Honorati, Paupy Christophe, Sumaye Robert, Prugnolle Franck. 2022. Molecular Ecology Resources, 22 (8) : 2915-2927.https://doi.org/10.1111/1755-0998.13670 <https://doi.org/10.1111/1755-0998.13670> Using haematophagous fly blood meals to study the diversity of blood-borne pathogens infecting wild mammals Mwakasungula, Solomon Rougeron, Virginie Arnathau, Céline Boundenga, Larson Miguel, Eve Boissiere, Anne Jiolle, Davy Durand, Patrick Msigwa, Alphonce Mswata, Sarah Olotu, Ally Sterkers, Yvon Roche, Benjamin Killeen, Gerard Cerqueira, Frédérique Bitome-Essono, Paul Yannick Bretagnolle, François Masanja, Honorati Paupy, Christophe Sumaye, Robert Prugnolle, Franck eng 2022 Molecular Ecology Resources transmission des maladies animal sauvage relation hôte pathogène agent pathogène écologie animale surveillance épidémiologique viande de gibier maladie infectieuse maladie des animaux zoonose virus peste porcine africaine identification Glossina sérologie http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_34017 http://aims.fao.org/aos/agrovoc/c_5630 http://aims.fao.org/aos/agrovoc/c_427 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_3183 http://aims.fao.org/aos/agrovoc/c_34024 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_8649 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_11174 http://aims.fao.org/aos/agrovoc/c_27081 République-Unie de Tanzanie http://aims.fao.org/aos/agrovoc/c_7608 Many emerging infectious diseases originate from wild animals, so there is a profound need for surveillance and monitoring of their pathogens. However, the practical difficulty of sample acquisition from wild animals tends to limit the feasibility and effectiveness of such surveys. Xenosurveillance, using blood-feeding invertebrates to obtain tissue samples from wild animals and then detect their pathogens, is a promising method to do so. Here, we describe the use of tsetse fly blood meals to determine (directly through molecular diagnostic and indirectly through serology), the diversity of circulating blood-borne pathogens (including bacteria, viruses and protozoa) in a natural mammalian community of Tanzania. Molecular analyses of captured tsetse flies (182 pools of flies totalizing 1728 flies) revealed that the blood meals obtained came from 18 different vertebrate species including 16 non-human mammals, representing approximately 25% of the large mammal species present in the study area. Molecular diagnostic demonstrated the presence of different protozoa parasites and bacteria of medical and/or veterinary interest. None of the six virus species searched for by molecular methods were detected but an ELISA test detected antibodies against African swine fever virus among warthogs, indicating that the virus had been circulating in the area. Sampling of blood-feeding insects represents an efficient and practical approach to tracking a diversity of pathogens from multiple mammalian species, directly through molecular diagnostic or indirectly through serology, which could readily expand and enhance our understanding of the ecology and evolution of infectious agents and their interactions with their hosts in wild animal communities. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/605750/1/Mwankasungula%20et%20al%2C%202022.pdf text cc_by_nc_nd info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ https://doi.org/10.1111/1755-0998.13670 10.1111/1755-0998.13670 info:eu-repo/semantics/altIdentifier/doi/10.1111/1755-0998.13670 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/1755-0998.13670 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA830652 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/ON652617 info:eu-repo/grantAgreement/EC/H2020/734548//(EU) A global alliance for Zika virus control and prevention/ZIKAlliance info:eu-repo/grantAgreement/EC/H2020/ANR-10-LABX-0004//(FRA) Mediterranean Center for Environment and Biodiversity/CeMEB info:eu-repo/grantAgreement/EC/H2020/ANR-11-LABX-0024//(FRA) Alliance française contre les maladies parasitaires/ParaFrap |