Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS)

A fast and low-cost analytical method to determine the concentrations of carotenoids (β-carotene, lutein, and lycopene) from mixed standard solutions or from fruit extracts (kiwi fruits, tomato paste, pink grapefruit juice, kiwi-pineapple smoothie, and apricot nectar) was tested. The methodology was based on UV-Vis spectrophotometry and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS). Results showed that relative concentrations of β-carotene, lutein, and lycopene in solvent were successfully determined by this technique with an error inferior to 6%. In real extracts, the procedure succeeded well in identifying the major carotenoid type of the fruit samples but also a more complex profile of a fruit mixture. The results also showed that accuracy of carotenoids determination by UV-Vis spectrophotometry-MCR-ALS in fruit extracts was conditioned by their spectral characteristics (III/II ratios and λmax), their relative proportion, and the extract purity.

Bibliographic Details

Main Authors:	Achir, Nawel, Servent, Adrien, Soto, Marvin, Dhuique-Mayer, Claudie
Format:	article biblioteca
Language:	eng
Subjects:	Q04 - Composition des produits alimentaires, Q01 - Sciences et technologies alimentaires - Considérations générales, composition des aliments, analyse quantitative, caroténoïde, extrait de fruit, spectrophotométrie, http://aims.fao.org/aos/agrovoc/c_10961, http://aims.fao.org/aos/agrovoc/c_32660, http://aims.fao.org/aos/agrovoc/c_1333, http://aims.fao.org/aos/agrovoc/c_29024, http://aims.fao.org/aos/agrovoc/c_14497,
Online Access:	http://agritrop.cirad.fr/600261/ http://agritrop.cirad.fr/600261/1/MCR%20Nawel-Hindawi.pdf
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