The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth
This study focused on the genetic, symbiotic and phenotypic diversity of a collection of 12 Vachellia seyal and Prosopis juliflora rhizobial strains that were isolated from soils influenced by the roots of the grass Sporobolus robustus. Phylogenetic trees were constructed based on data from the 16S rRNA gene, from a multi-locus sequence analysis (MLSA) of the housekeeping genes recA, atpD, glnA and gyrB, as well as from the symbiotic genes nodA, nodC and nifH. Most of the mesorhizobial strains were clustered with the M. plurifarium type strain ORS 1032T and the 16S rRNA gene was as discriminatory as the other housekeeping genes. The Ensifer collection was closely related to E. alkalisoli YIC 4027T and E. fredii LMG 6217T using 16S rRNA and MLSA. Amplification of the Rhizobium collection was only observed with the 16S rRNA gene, where the strains were clustered with Rhizobium sp. 1B, Rhizobium sp. SEMIA 6411, Rhizobium sp. AS1-101a and Rhizobium sp. ORS 3441. The phylogeny of the nifH gene from Mesorhizobium and Ensifer strains were similar to those of nodulation genes (nodA and nodC). The nodA-nodC and nifH gene phylogenies were not consistent with those of 16S rRNA and the housekeeping genes. Ensifer genospecies tolerated high salinity better than the other genotypes after testing on a salt content gradient up to 49‰. We conclude that the rhizosphere of Sporobolus tussocks harbors a rhizobial microbiota, which could be capable of helping V. seyal and P. juliflora to grow better in saline soils.
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dig-cirad-fr-5993152024-01-29T03:47:11Z http://agritrop.cirad.fr/599315/ http://agritrop.cirad.fr/599315/ The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth. Fall Fatoumata, Le Roux Christine, Bâ Amadou Moustapha, Fall Dioumacor, Bakhoum Niokhor, Faye Mathieu Ndigue, Sadio Oumar, Diouf Diégane. 2021. Symbiosis, 84 : 61-69.https://doi.org/10.1007/s13199-021-00763-7 <https://doi.org/10.1007/s13199-021-00763-7> The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth Fall, Fatoumata Le Roux, Christine Bâ, Amadou Moustapha Fall, Dioumacor Bakhoum, Niokhor Faye, Mathieu Ndigue Sadio, Oumar Diouf, Diégane eng 2021 Symbiosis F61 - Physiologie végétale - Nutrition P34 - Biologie du sol F30 - Génétique et amélioration des plantes Mimosoideae Prosopis juliflora symbiose symbiote Sporobolus Rhizobium biodiversité variation génétique phylogénie tolérance au sel arn messager http://aims.fao.org/aos/agrovoc/c_4846 http://aims.fao.org/aos/agrovoc/c_15891 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_7325 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4765 Sénégal http://aims.fao.org/aos/agrovoc/c_6970 This study focused on the genetic, symbiotic and phenotypic diversity of a collection of 12 Vachellia seyal and Prosopis juliflora rhizobial strains that were isolated from soils influenced by the roots of the grass Sporobolus robustus. Phylogenetic trees were constructed based on data from the 16S rRNA gene, from a multi-locus sequence analysis (MLSA) of the housekeeping genes recA, atpD, glnA and gyrB, as well as from the symbiotic genes nodA, nodC and nifH. Most of the mesorhizobial strains were clustered with the M. plurifarium type strain ORS 1032T and the 16S rRNA gene was as discriminatory as the other housekeeping genes. The Ensifer collection was closely related to E. alkalisoli YIC 4027T and E. fredii LMG 6217T using 16S rRNA and MLSA. Amplification of the Rhizobium collection was only observed with the 16S rRNA gene, where the strains were clustered with Rhizobium sp. 1B, Rhizobium sp. SEMIA 6411, Rhizobium sp. AS1-101a and Rhizobium sp. ORS 3441. The phylogeny of the nifH gene from Mesorhizobium and Ensifer strains were similar to those of nodulation genes (nodA and nodC). The nodA-nodC and nifH gene phylogenies were not consistent with those of 16S rRNA and the housekeeping genes. Ensifer genospecies tolerated high salinity better than the other genotypes after testing on a salt content gradient up to 49‰. We conclude that the rhizosphere of Sporobolus tussocks harbors a rhizobial microbiota, which could be capable of helping V. seyal and P. juliflora to grow better in saline soils. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/599315/1/Fall%202021%20The%20leguminous%20trees%20Vachellia%20seyal%20%28Del.%29%20and%20Prosopis%20juliflora%20%28Swartz%29%20DC%20and%20their%20association%20with%20rhizobial%20strains%20from%20the%20root-influence%20zone%20of%20the%20grass%20Sporobolus%20robustus%20Kunth.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s13199-021-00763-7 10.1007/s13199-021-00763-7 info:eu-repo/semantics/altIdentifier/doi/10.1007/s13199-021-00763-7 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s13199-021-00763-7 |
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F61 - Physiologie végétale - Nutrition P34 - Biologie du sol F30 - Génétique et amélioration des plantes Mimosoideae Prosopis juliflora symbiose symbiote Sporobolus Rhizobium biodiversité variation génétique phylogénie tolérance au sel arn messager http://aims.fao.org/aos/agrovoc/c_4846 http://aims.fao.org/aos/agrovoc/c_15891 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_7325 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_6970 F61 - Physiologie végétale - Nutrition P34 - Biologie du sol F30 - Génétique et amélioration des plantes Mimosoideae Prosopis juliflora symbiose symbiote Sporobolus Rhizobium biodiversité variation génétique phylogénie tolérance au sel arn messager http://aims.fao.org/aos/agrovoc/c_4846 http://aims.fao.org/aos/agrovoc/c_15891 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_7325 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_6970 |
spellingShingle |
F61 - Physiologie végétale - Nutrition P34 - Biologie du sol F30 - Génétique et amélioration des plantes Mimosoideae Prosopis juliflora symbiose symbiote Sporobolus Rhizobium biodiversité variation génétique phylogénie tolérance au sel arn messager http://aims.fao.org/aos/agrovoc/c_4846 http://aims.fao.org/aos/agrovoc/c_15891 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_7325 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_6970 F61 - Physiologie végétale - Nutrition P34 - Biologie du sol F30 - Génétique et amélioration des plantes Mimosoideae Prosopis juliflora symbiose symbiote Sporobolus Rhizobium biodiversité variation génétique phylogénie tolérance au sel arn messager http://aims.fao.org/aos/agrovoc/c_4846 http://aims.fao.org/aos/agrovoc/c_15891 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_7325 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_6970 Fall, Fatoumata Le Roux, Christine Bâ, Amadou Moustapha Fall, Dioumacor Bakhoum, Niokhor Faye, Mathieu Ndigue Sadio, Oumar Diouf, Diégane The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth |
description |
This study focused on the genetic, symbiotic and phenotypic diversity of a collection of 12 Vachellia seyal and Prosopis juliflora rhizobial strains that were isolated from soils influenced by the roots of the grass Sporobolus robustus. Phylogenetic trees were constructed based on data from the 16S rRNA gene, from a multi-locus sequence analysis (MLSA) of the housekeeping genes recA, atpD, glnA and gyrB, as well as from the symbiotic genes nodA, nodC and nifH. Most of the mesorhizobial strains were clustered with the M. plurifarium type strain ORS 1032T and the 16S rRNA gene was as discriminatory as the other housekeeping genes. The Ensifer collection was closely related to E. alkalisoli YIC 4027T and E. fredii LMG 6217T using 16S rRNA and MLSA. Amplification of the Rhizobium collection was only observed with the 16S rRNA gene, where the strains were clustered with Rhizobium sp. 1B, Rhizobium sp. SEMIA 6411, Rhizobium sp. AS1-101a and Rhizobium sp. ORS 3441. The phylogeny of the nifH gene from Mesorhizobium and Ensifer strains were similar to those of nodulation genes (nodA and nodC). The nodA-nodC and nifH gene phylogenies were not consistent with those of 16S rRNA and the housekeeping genes. Ensifer genospecies tolerated high salinity better than the other genotypes after testing on a salt content gradient up to 49‰. We conclude that the rhizosphere of Sporobolus tussocks harbors a rhizobial microbiota, which could be capable of helping V. seyal and P. juliflora to grow better in saline soils. |
format |
article |
topic_facet |
F61 - Physiologie végétale - Nutrition P34 - Biologie du sol F30 - Génétique et amélioration des plantes Mimosoideae Prosopis juliflora symbiose symbiote Sporobolus Rhizobium biodiversité variation génétique phylogénie tolérance au sel arn messager http://aims.fao.org/aos/agrovoc/c_4846 http://aims.fao.org/aos/agrovoc/c_15891 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_7325 http://aims.fao.org/aos/agrovoc/c_6563 http://aims.fao.org/aos/agrovoc/c_33949 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_6970 |
author |
Fall, Fatoumata Le Roux, Christine Bâ, Amadou Moustapha Fall, Dioumacor Bakhoum, Niokhor Faye, Mathieu Ndigue Sadio, Oumar Diouf, Diégane |
author_facet |
Fall, Fatoumata Le Roux, Christine Bâ, Amadou Moustapha Fall, Dioumacor Bakhoum, Niokhor Faye, Mathieu Ndigue Sadio, Oumar Diouf, Diégane |
author_sort |
Fall, Fatoumata |
title |
The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth |
title_short |
The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth |
title_full |
The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth |
title_fullStr |
The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth |
title_full_unstemmed |
The leguminous trees Vachellia seyal (Del.) and Prosopis juliflora (Swartz) DC and their association with rhizobial strains from the root-influence zone of the grass Sporobolus robustus Kunth |
title_sort |
leguminous trees vachellia seyal (del.) and prosopis juliflora (swartz) dc and their association with rhizobial strains from the root-influence zone of the grass sporobolus robustus kunth |
url |
http://agritrop.cirad.fr/599315/ http://agritrop.cirad.fr/599315/1/Fall%202021%20The%20leguminous%20trees%20Vachellia%20seyal%20%28Del.%29%20and%20Prosopis%20juliflora%20%28Swartz%29%20DC%20and%20their%20association%20with%20rhizobial%20strains%20from%20the%20root-influence%20zone%20of%20the%20grass%20Sporobolus%20robustus%20Kunth.pdf |
work_keys_str_mv |
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