Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904)
Melanaphis sacchari (Zehntner, 1897) and Melanaphis sorghi (Theobald, 1904) are major worldwide crop pests causing direct feeding damage on sorghum and transmitting viruses to sugarcane. It is common in the scientific literature to consider these two species as synonyms, referred to as the 'sugarcane aphid', although no formal study has validated this synonymy. In this study, based on the comparison of samples collected from their whole distribution area, we use both morphometric and molecular data to better characterize the discrimination between M. sacchari and M. sorghi. An unsupervised multivariate analysis of morphometric data clearly confirmed the separation of the two species. The best discriminating characters separating these species were length of the antenna processus terminalis relative to length of hind tibia, siphunculus or cauda. However, those criteria sometimes do not allow an unambiguous identification. Bayesian clustering based on microsatellite data delimited two clusters, which corresponded to the morphological species separation. The DNA sequencing of three nuclear and three mitochondrial regions revealed slight divergence between species. In particular, the COI barcode region proved to be uninformative for species separation because one haplotype is shared by both species. In contrast, one SNP located on the nuclear EF1-α gene was diagnostic for species separation. Based on morphological and molecular evidence, the invasive genotype damaging to sorghum in the US, Mexico and the Caribbean since 2013 is found to be M. sorghi.
Main Authors: | , , , , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H10 - Ravageurs des plantes, H50 - Troubles divers des plantes, ravageur des plantes, taxonomie, insecte nuisible, morphologie animale, distribution des populations, Aphididae, Saccharum officinarum, Sorghum, analyse discriminante, http://aims.fao.org/aos/agrovoc/c_16196, http://aims.fao.org/aos/agrovoc/c_7631, http://aims.fao.org/aos/agrovoc/c_5729, http://aims.fao.org/aos/agrovoc/c_421, http://aims.fao.org/aos/agrovoc/c_6113, http://aims.fao.org/aos/agrovoc/c_525, http://aims.fao.org/aos/agrovoc/c_6727, http://aims.fao.org/aos/agrovoc/c_7244, http://aims.fao.org/aos/agrovoc/c_28859, |
Online Access: | http://agritrop.cirad.fr/597964/ http://agritrop.cirad.fr/597964/1/Nibouche%20et%20al.%202021.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-597964 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5979642024-04-24T11:50:48Z http://agritrop.cirad.fr/597964/ http://agritrop.cirad.fr/597964/ Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904). Nibouche Samuel, Costet Laurent, Medina Raul F., Holt Jocelyn R., Sadeyen Joëlle, Zoogones Anne-Sophie, Brown Paul, Blackman Roger L.. 2021. PloS One, 16 (3):e0241881, 17 p.https://doi.org/10.1371/journal.pone.0241881 <https://doi.org/10.1371/journal.pone.0241881> Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) Nibouche, Samuel Costet, Laurent Medina, Raul F. Holt, Jocelyn R. Sadeyen, Joëlle Zoogones, Anne-Sophie Brown, Paul Blackman, Roger L. eng 2021 PloS One H10 - Ravageurs des plantes H50 - Troubles divers des plantes ravageur des plantes taxonomie insecte nuisible morphologie animale distribution des populations Aphididae Saccharum officinarum Sorghum analyse discriminante http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_5729 http://aims.fao.org/aos/agrovoc/c_421 http://aims.fao.org/aos/agrovoc/c_6113 http://aims.fao.org/aos/agrovoc/c_525 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_28859 Melanaphis sacchari (Zehntner, 1897) and Melanaphis sorghi (Theobald, 1904) are major worldwide crop pests causing direct feeding damage on sorghum and transmitting viruses to sugarcane. It is common in the scientific literature to consider these two species as synonyms, referred to as the 'sugarcane aphid', although no formal study has validated this synonymy. In this study, based on the comparison of samples collected from their whole distribution area, we use both morphometric and molecular data to better characterize the discrimination between M. sacchari and M. sorghi. An unsupervised multivariate analysis of morphometric data clearly confirmed the separation of the two species. The best discriminating characters separating these species were length of the antenna processus terminalis relative to length of hind tibia, siphunculus or cauda. However, those criteria sometimes do not allow an unambiguous identification. Bayesian clustering based on microsatellite data delimited two clusters, which corresponded to the morphological species separation. The DNA sequencing of three nuclear and three mitochondrial regions revealed slight divergence between species. In particular, the COI barcode region proved to be uninformative for species separation because one haplotype is shared by both species. In contrast, one SNP located on the nuclear EF1-α gene was diagnostic for species separation. Based on morphological and molecular evidence, the invasive genotype damaging to sorghum in the US, Mexico and the Caribbean since 2013 is found to be M. sorghi. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/597964/1/Nibouche%20et%20al.%202021.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1371/journal.pone.0241881 10.1371/journal.pone.0241881 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0241881 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0241881 info:eu-repo/semantics/reference/purl/https://doi.org/10.18167/DVN1/PDPDS4 info:eu-repo/semantics/reference/purl/https://github.com/SamNibouche/Melanaphis_taxonomy info:eu-repo/semantics/dataset/purl/http://arthemisdb.supagro.inra.fr/ info:eu-repo/grantAgreement/EC///// |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H10 - Ravageurs des plantes H50 - Troubles divers des plantes ravageur des plantes taxonomie insecte nuisible morphologie animale distribution des populations Aphididae Saccharum officinarum Sorghum analyse discriminante http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_5729 http://aims.fao.org/aos/agrovoc/c_421 http://aims.fao.org/aos/agrovoc/c_6113 http://aims.fao.org/aos/agrovoc/c_525 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_28859 H10 - Ravageurs des plantes H50 - Troubles divers des plantes ravageur des plantes taxonomie insecte nuisible morphologie animale distribution des populations Aphididae Saccharum officinarum Sorghum analyse discriminante http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_5729 http://aims.fao.org/aos/agrovoc/c_421 http://aims.fao.org/aos/agrovoc/c_6113 http://aims.fao.org/aos/agrovoc/c_525 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_28859 |
spellingShingle |
H10 - Ravageurs des plantes H50 - Troubles divers des plantes ravageur des plantes taxonomie insecte nuisible morphologie animale distribution des populations Aphididae Saccharum officinarum Sorghum analyse discriminante http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_5729 http://aims.fao.org/aos/agrovoc/c_421 http://aims.fao.org/aos/agrovoc/c_6113 http://aims.fao.org/aos/agrovoc/c_525 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_28859 H10 - Ravageurs des plantes H50 - Troubles divers des plantes ravageur des plantes taxonomie insecte nuisible morphologie animale distribution des populations Aphididae Saccharum officinarum Sorghum analyse discriminante http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_5729 http://aims.fao.org/aos/agrovoc/c_421 http://aims.fao.org/aos/agrovoc/c_6113 http://aims.fao.org/aos/agrovoc/c_525 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_28859 Nibouche, Samuel Costet, Laurent Medina, Raul F. Holt, Jocelyn R. Sadeyen, Joëlle Zoogones, Anne-Sophie Brown, Paul Blackman, Roger L. Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) |
description |
Melanaphis sacchari (Zehntner, 1897) and Melanaphis sorghi (Theobald, 1904) are major worldwide crop pests causing direct feeding damage on sorghum and transmitting viruses to sugarcane. It is common in the scientific literature to consider these two species as synonyms, referred to as the 'sugarcane aphid', although no formal study has validated this synonymy. In this study, based on the comparison of samples collected from their whole distribution area, we use both morphometric and molecular data to better characterize the discrimination between M. sacchari and M. sorghi. An unsupervised multivariate analysis of morphometric data clearly confirmed the separation of the two species. The best discriminating characters separating these species were length of the antenna processus terminalis relative to length of hind tibia, siphunculus or cauda. However, those criteria sometimes do not allow an unambiguous identification. Bayesian clustering based on microsatellite data delimited two clusters, which corresponded to the morphological species separation. The DNA sequencing of three nuclear and three mitochondrial regions revealed slight divergence between species. In particular, the COI barcode region proved to be uninformative for species separation because one haplotype is shared by both species. In contrast, one SNP located on the nuclear EF1-α gene was diagnostic for species separation. Based on morphological and molecular evidence, the invasive genotype damaging to sorghum in the US, Mexico and the Caribbean since 2013 is found to be M. sorghi. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes H50 - Troubles divers des plantes ravageur des plantes taxonomie insecte nuisible morphologie animale distribution des populations Aphididae Saccharum officinarum Sorghum analyse discriminante http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_5729 http://aims.fao.org/aos/agrovoc/c_421 http://aims.fao.org/aos/agrovoc/c_6113 http://aims.fao.org/aos/agrovoc/c_525 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_7244 http://aims.fao.org/aos/agrovoc/c_28859 |
author |
Nibouche, Samuel Costet, Laurent Medina, Raul F. Holt, Jocelyn R. Sadeyen, Joëlle Zoogones, Anne-Sophie Brown, Paul Blackman, Roger L. |
author_facet |
Nibouche, Samuel Costet, Laurent Medina, Raul F. Holt, Jocelyn R. Sadeyen, Joëlle Zoogones, Anne-Sophie Brown, Paul Blackman, Roger L. |
author_sort |
Nibouche, Samuel |
title |
Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) |
title_short |
Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) |
title_full |
Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) |
title_fullStr |
Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) |
title_full_unstemmed |
Morphometric and molecular discrimination of the sugarcane aphid, Melanaphis sacchari, (Zehntner, 1897) and the sorghum aphid Melanaphis sorghi (Theobald, 1904) |
title_sort |
morphometric and molecular discrimination of the sugarcane aphid, melanaphis sacchari, (zehntner, 1897) and the sorghum aphid melanaphis sorghi (theobald, 1904) |
url |
http://agritrop.cirad.fr/597964/ http://agritrop.cirad.fr/597964/1/Nibouche%20et%20al.%202021.pdf |
work_keys_str_mv |
AT nibouchesamuel morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT costetlaurent morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT medinaraulf morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT holtjocelynr morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT sadeyenjoelle morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT zoogonesannesophie morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT brownpaul morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 AT blackmanrogerl morphometricandmoleculardiscriminationofthesugarcaneaphidmelanaphissaccharizehntner1897andthesorghumaphidmelanaphissorghitheobald1904 |
_version_ |
1798165061049516032 |