Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes
The measurement of virus-specific neutralising antibodies represents the “gold-standard” for diagnostic serology. For animal morbilliviruses, such as peste des petits ruminants (PPRV) or rinderpest virus (RPV), live virus-based neutralisation tests require high-level biocontainment to prevent the accidental escape of the infectious agents. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSVΔG) based pseudotyping system for the measurement of neutralising antibodies against animal morbilliviruses. By expressing the haemagglutinin (H) and fusion (F) proteins of PPRV on VSVΔG pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Serological responses against the four distinct lineages of PPRV could be measured simultaneously and cross-neutralising responses against other morbilliviruses compared. Using this approach, we observed that titres of neutralising antibodies induced by vaccination with live attenuated PPRV were lower than those induced by wild type virus infection and the level of cross-lineage neutralisation varied between vaccinates. By comparing neutralising responses from animals infected with either PPRV or RPV, we found that responses were highest against the homologous virus, indicating that retrospective analyses of serum samples could be used to confirm the nature of the original pathogen to which an animal had been exposed. Accordingly, when screening sera from domestic livestock and wild ruminants in Tanzania, we detected evidence of cross-species infection with PPRV, canine distemper virus (CDV) and a RPV-related bovine morbillivirus, suggesting that exposure to animal morbilliviruses may be more widespread than indicated previously using existing diagnostic techniques.
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Language: | eng |
Subjects: | L73 - Maladies des animaux, L70 - Sciences et hygiène vétérinaires - Considérations générales, morbillivirus, immunologie, anticorps, virus stomatite vésiculeuse, immunopathologie, virus peste petits ruminants, virus peste bovine, sérologie, immunodiagnostic, http://aims.fao.org/aos/agrovoc/c_4937, http://aims.fao.org/aos/agrovoc/c_3808, http://aims.fao.org/aos/agrovoc/c_493, http://aims.fao.org/aos/agrovoc/c_15158, http://aims.fao.org/aos/agrovoc/c_29628, http://aims.fao.org/aos/agrovoc/c_16581, http://aims.fao.org/aos/agrovoc/c_13928, http://aims.fao.org/aos/agrovoc/c_27081, http://aims.fao.org/aos/agrovoc/c_27555, |
Online Access: | http://agritrop.cirad.fr/597694/ http://agritrop.cirad.fr/597694/1/597694.pdf |
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dig-cirad-fr-5976942024-01-29T03:21:34Z http://agritrop.cirad.fr/597694/ http://agritrop.cirad.fr/597694/ Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes. Logan Nicola, Dundon William G., Diallo Adama, Baron Michael D., Nyarobi M. James, Cleaveland Sarah, Keyyu Julius, Fyumagwa Robert, Hosie Margaret J., Willett Brian J.. 2016. Vaccine, 34 (47) : 5736-5743.https://doi.org/10.1016/j.vaccine.2016.10.010 <https://doi.org/10.1016/j.vaccine.2016.10.010> Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes Logan, Nicola Dundon, William G. Diallo, Adama Baron, Michael D. Nyarobi, M. James Cleaveland, Sarah Keyyu, Julius Fyumagwa, Robert Hosie, Margaret J. Willett, Brian J. eng 2016 Vaccine L73 - Maladies des animaux L70 - Sciences et hygiène vétérinaires - Considérations générales morbillivirus immunologie anticorps virus stomatite vésiculeuse immunopathologie virus peste petits ruminants virus peste bovine sérologie immunodiagnostic http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_15158 http://aims.fao.org/aos/agrovoc/c_29628 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_13928 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_27555 The measurement of virus-specific neutralising antibodies represents the “gold-standard” for diagnostic serology. For animal morbilliviruses, such as peste des petits ruminants (PPRV) or rinderpest virus (RPV), live virus-based neutralisation tests require high-level biocontainment to prevent the accidental escape of the infectious agents. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSVΔG) based pseudotyping system for the measurement of neutralising antibodies against animal morbilliviruses. By expressing the haemagglutinin (H) and fusion (F) proteins of PPRV on VSVΔG pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Serological responses against the four distinct lineages of PPRV could be measured simultaneously and cross-neutralising responses against other morbilliviruses compared. Using this approach, we observed that titres of neutralising antibodies induced by vaccination with live attenuated PPRV were lower than those induced by wild type virus infection and the level of cross-lineage neutralisation varied between vaccinates. By comparing neutralising responses from animals infected with either PPRV or RPV, we found that responses were highest against the homologous virus, indicating that retrospective analyses of serum samples could be used to confirm the nature of the original pathogen to which an animal had been exposed. Accordingly, when screening sera from domestic livestock and wild ruminants in Tanzania, we detected evidence of cross-species infection with PPRV, canine distemper virus (CDV) and a RPV-related bovine morbillivirus, suggesting that exposure to animal morbilliviruses may be more widespread than indicated previously using existing diagnostic techniques. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/597694/1/597694.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1016/j.vaccine.2016.10.010 10.1016/j.vaccine.2016.10.010 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2016.10.010 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.vaccine.2016.10.010 |
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L73 - Maladies des animaux L70 - Sciences et hygiène vétérinaires - Considérations générales morbillivirus immunologie anticorps virus stomatite vésiculeuse immunopathologie virus peste petits ruminants virus peste bovine sérologie immunodiagnostic http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_15158 http://aims.fao.org/aos/agrovoc/c_29628 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_13928 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_27555 L73 - Maladies des animaux L70 - Sciences et hygiène vétérinaires - Considérations générales morbillivirus immunologie anticorps virus stomatite vésiculeuse immunopathologie virus peste petits ruminants virus peste bovine sérologie immunodiagnostic http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_15158 http://aims.fao.org/aos/agrovoc/c_29628 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_13928 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_27555 |
spellingShingle |
L73 - Maladies des animaux L70 - Sciences et hygiène vétérinaires - Considérations générales morbillivirus immunologie anticorps virus stomatite vésiculeuse immunopathologie virus peste petits ruminants virus peste bovine sérologie immunodiagnostic http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_15158 http://aims.fao.org/aos/agrovoc/c_29628 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_13928 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_27555 L73 - Maladies des animaux L70 - Sciences et hygiène vétérinaires - Considérations générales morbillivirus immunologie anticorps virus stomatite vésiculeuse immunopathologie virus peste petits ruminants virus peste bovine sérologie immunodiagnostic http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_15158 http://aims.fao.org/aos/agrovoc/c_29628 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_13928 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_27555 Logan, Nicola Dundon, William G. Diallo, Adama Baron, Michael D. Nyarobi, M. James Cleaveland, Sarah Keyyu, Julius Fyumagwa, Robert Hosie, Margaret J. Willett, Brian J. Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes |
description |
The measurement of virus-specific neutralising antibodies represents the “gold-standard” for diagnostic serology. For animal morbilliviruses, such as peste des petits ruminants (PPRV) or rinderpest virus (RPV), live virus-based neutralisation tests require high-level biocontainment to prevent the accidental escape of the infectious agents. In this study, we describe the adaptation of a replication-defective vesicular stomatitis virus (VSVΔG) based pseudotyping system for the measurement of neutralising antibodies against animal morbilliviruses. By expressing the haemagglutinin (H) and fusion (F) proteins of PPRV on VSVΔG pseudotypes bearing a luciferase marker gene, neutralising antibody titres could be measured rapidly and with high sensitivity. Serological responses against the four distinct lineages of PPRV could be measured simultaneously and cross-neutralising responses against other morbilliviruses compared. Using this approach, we observed that titres of neutralising antibodies induced by vaccination with live attenuated PPRV were lower than those induced by wild type virus infection and the level of cross-lineage neutralisation varied between vaccinates. By comparing neutralising responses from animals infected with either PPRV or RPV, we found that responses were highest against the homologous virus, indicating that retrospective analyses of serum samples could be used to confirm the nature of the original pathogen to which an animal had been exposed. Accordingly, when screening sera from domestic livestock and wild ruminants in Tanzania, we detected evidence of cross-species infection with PPRV, canine distemper virus (CDV) and a RPV-related bovine morbillivirus, suggesting that exposure to animal morbilliviruses may be more widespread than indicated previously using existing diagnostic techniques. |
format |
article |
topic_facet |
L73 - Maladies des animaux L70 - Sciences et hygiène vétérinaires - Considérations générales morbillivirus immunologie anticorps virus stomatite vésiculeuse immunopathologie virus peste petits ruminants virus peste bovine sérologie immunodiagnostic http://aims.fao.org/aos/agrovoc/c_4937 http://aims.fao.org/aos/agrovoc/c_3808 http://aims.fao.org/aos/agrovoc/c_493 http://aims.fao.org/aos/agrovoc/c_15158 http://aims.fao.org/aos/agrovoc/c_29628 http://aims.fao.org/aos/agrovoc/c_16581 http://aims.fao.org/aos/agrovoc/c_13928 http://aims.fao.org/aos/agrovoc/c_27081 http://aims.fao.org/aos/agrovoc/c_27555 |
author |
Logan, Nicola Dundon, William G. Diallo, Adama Baron, Michael D. Nyarobi, M. James Cleaveland, Sarah Keyyu, Julius Fyumagwa, Robert Hosie, Margaret J. Willett, Brian J. |
author_facet |
Logan, Nicola Dundon, William G. Diallo, Adama Baron, Michael D. Nyarobi, M. James Cleaveland, Sarah Keyyu, Julius Fyumagwa, Robert Hosie, Margaret J. Willett, Brian J. |
author_sort |
Logan, Nicola |
title |
Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes |
title_short |
Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes |
title_full |
Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes |
title_fullStr |
Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes |
title_full_unstemmed |
Enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (VSV) pseudotypes |
title_sort |
enhanced immunosurveillance for animal morbilliviruses using vesicular stomatitis virus (vsv) pseudotypes |
url |
http://agritrop.cirad.fr/597694/ http://agritrop.cirad.fr/597694/1/597694.pdf |
work_keys_str_mv |
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