Optimization of a protein extraction method from natural rubber sheets made of Hevea brasiliensis latex

A method to extract proteins from natural rubber (NR) samples has been developed to characterize the protein composition of NR by SDS-PAGE electrophoresis. While the incubation of NR pieces in aqueous buffers did not provide satisfactory results (no protein bands detected by SDS-PAGE), the solubilization of NR pieces in an organic solvent, prior to centrifugation and protein extraction from the obtained pellet, was found efficient to highlight protein bands by SDS-PAGE. Pure cyclohexane was used to solubilize NR pieces and resulted in a low share of nitrogen in the pellet (29%). The addition of 10% (v/v) ethanol to cyclohexane allowed to significantly increase this share to 73% and to detect 13 protein bands by SDS-PAGE. The molecular weights of the four most intense bands suggest the presence in NR of the following proteins: rubber elongation factor, small rubber particle protein, hevamine, and β-1-3 glucanase.

Bibliographic Details

Main Authors:	Srisomboon, Surattiya, Wadeesirisak, Kanthida, Vaysse, Laurent, Sainte-Beuve, Jérôme, Musigamart, Natedao, Liengprayoon, Siriluck, Bonfils, Frédéric, Rattanaporn, Kittipong, Bottier, Céline
Format:	article biblioteca
Language:	eng
Subjects:	Q60 - Traitement des produits agricoles non alimentaires, U50 - Sciences physiques et chimie, Hevea brasiliensis, extraction, caoutchouc, électrophorèse gel polyacrylamide, qualité protéique, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_36910, http://aims.fao.org/aos/agrovoc/c_6678, http://aims.fao.org/aos/agrovoc/c_34078, http://aims.fao.org/aos/agrovoc/c_6257,
Online Access:	http://agritrop.cirad.fr/597312/ http://agritrop.cirad.fr/597312/7/597312.pdf
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