Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage
The impact of NH3-stabilization of latex on its biochemical and physicochemical indicators at various storage times (0–41 days) was investigated. Liquid latex was characterized by pH, dry rubber content, total solid content, surface tension, lipid and protein contents. In addition, lipid and protein profiles were monitored by TLC and SDS-PAGE, respectively. Thin latex films were studied by AFM to unravel their morphology, stiffness and adhesion, whereas infrared and wettability measurements provided information on composition and wetting properties. NH3-stabilization method was shown to rapidly (12 days) and significantly impact lipid and protein contents resulting in drastic changes in the morphology as well as in the mechanical and physicochemical properties of films. The presence of a layer of very high stiffness (GPa) on the rubber surface was noticed which was hypothesized to contain REF1 and SRPP1, the two major latex proteins. This was later confirmed by imaging a film of dry latex extract, known to contain both REF1 and SRPP1 proteins, which revealed stiffness of the order of GPa. As a protein known for its amyloid-forming capacity, REF1 could be the main component responsible for GPa-range stiffness of NH3-stabilized films and might then play a crucial role on the final properties of natural rubber.
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Subjects: | J11 - Manutention, transport, stockage et conservation des produits d'origine végétale, F60 - Physiologie et biochimie végétale, Hevea brasiliensis, latex, stockage, caoutchouc, propriété physicochimique, protéine, http://aims.fao.org/aos/agrovoc/c_3589, http://aims.fao.org/aos/agrovoc/c_4214, http://aims.fao.org/aos/agrovoc/c_7427, http://aims.fao.org/aos/agrovoc/c_6678, http://aims.fao.org/aos/agrovoc/c_1521, http://aims.fao.org/aos/agrovoc/c_6259, http://aims.fao.org/aos/agrovoc/c_7701, |
Online Access: | http://agritrop.cirad.fr/591710/ http://agritrop.cirad.fr/591710/1/Coll%20Surf%20A_2019.pdf |
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dig-cirad-fr-5917102024-01-29T01:46:01Z http://agritrop.cirad.fr/591710/ http://agritrop.cirad.fr/591710/ Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage. Bottier Céline, Gross Bryan, Wadeesirisak Kanthida, Srisomboon Surattiya, Jantarasunthorn Saowalak, Musigamart Natedao, Roytrakul Sittiruk, Liengprayoon Siriluck, Vaysse Laurent, Kunemann Philippe, Vallat Marie-France, Mougin Karine. 2019. Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 570 : 487-498.https://doi.org/10.1016/j.colsurfa.2019.03.028 <https://doi.org/10.1016/j.colsurfa.2019.03.028> Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage Bottier, Céline Gross, Bryan Wadeesirisak, Kanthida Srisomboon, Surattiya Jantarasunthorn, Saowalak Musigamart, Natedao Roytrakul, Sittiruk Liengprayoon, Siriluck Vaysse, Laurent Kunemann, Philippe Vallat, Marie-France Mougin, Karine eng 2019 Colloids and Surfaces. A, Physicochemical and Engineering Aspects J11 - Manutention, transport, stockage et conservation des produits d'origine végétale F60 - Physiologie et biochimie végétale Hevea brasiliensis latex stockage caoutchouc propriété physicochimique protéine http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7427 http://aims.fao.org/aos/agrovoc/c_6678 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_6259 Thaïlande http://aims.fao.org/aos/agrovoc/c_7701 The impact of NH3-stabilization of latex on its biochemical and physicochemical indicators at various storage times (0–41 days) was investigated. Liquid latex was characterized by pH, dry rubber content, total solid content, surface tension, lipid and protein contents. In addition, lipid and protein profiles were monitored by TLC and SDS-PAGE, respectively. Thin latex films were studied by AFM to unravel their morphology, stiffness and adhesion, whereas infrared and wettability measurements provided information on composition and wetting properties. NH3-stabilization method was shown to rapidly (12 days) and significantly impact lipid and protein contents resulting in drastic changes in the morphology as well as in the mechanical and physicochemical properties of films. The presence of a layer of very high stiffness (GPa) on the rubber surface was noticed which was hypothesized to contain REF1 and SRPP1, the two major latex proteins. This was later confirmed by imaging a film of dry latex extract, known to contain both REF1 and SRPP1 proteins, which revealed stiffness of the order of GPa. As a protein known for its amyloid-forming capacity, REF1 could be the main component responsible for GPa-range stiffness of NH3-stabilized films and might then play a crucial role on the final properties of natural rubber. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/591710/1/Coll%20Surf%20A_2019.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.colsurfa.2019.03.028 10.1016/j.colsurfa.2019.03.028 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.colsurfa.2019.03.028 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.colsurfa.2019.03.028 |
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J11 - Manutention, transport, stockage et conservation des produits d'origine végétale F60 - Physiologie et biochimie végétale Hevea brasiliensis latex stockage caoutchouc propriété physicochimique protéine http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7427 http://aims.fao.org/aos/agrovoc/c_6678 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_7701 J11 - Manutention, transport, stockage et conservation des produits d'origine végétale F60 - Physiologie et biochimie végétale Hevea brasiliensis latex stockage caoutchouc propriété physicochimique protéine http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7427 http://aims.fao.org/aos/agrovoc/c_6678 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_7701 |
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J11 - Manutention, transport, stockage et conservation des produits d'origine végétale F60 - Physiologie et biochimie végétale Hevea brasiliensis latex stockage caoutchouc propriété physicochimique protéine http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7427 http://aims.fao.org/aos/agrovoc/c_6678 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_7701 J11 - Manutention, transport, stockage et conservation des produits d'origine végétale F60 - Physiologie et biochimie végétale Hevea brasiliensis latex stockage caoutchouc propriété physicochimique protéine http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7427 http://aims.fao.org/aos/agrovoc/c_6678 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_7701 Bottier, Céline Gross, Bryan Wadeesirisak, Kanthida Srisomboon, Surattiya Jantarasunthorn, Saowalak Musigamart, Natedao Roytrakul, Sittiruk Liengprayoon, Siriluck Vaysse, Laurent Kunemann, Philippe Vallat, Marie-France Mougin, Karine Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage |
description |
The impact of NH3-stabilization of latex on its biochemical and physicochemical indicators at various storage times (0–41 days) was investigated. Liquid latex was characterized by pH, dry rubber content, total solid content, surface tension, lipid and protein contents. In addition, lipid and protein profiles were monitored by TLC and SDS-PAGE, respectively. Thin latex films were studied by AFM to unravel their morphology, stiffness and adhesion, whereas infrared and wettability measurements provided information on composition and wetting properties. NH3-stabilization method was shown to rapidly (12 days) and significantly impact lipid and protein contents resulting in drastic changes in the morphology as well as in the mechanical and physicochemical properties of films. The presence of a layer of very high stiffness (GPa) on the rubber surface was noticed which was hypothesized to contain REF1 and SRPP1, the two major latex proteins. This was later confirmed by imaging a film of dry latex extract, known to contain both REF1 and SRPP1 proteins, which revealed stiffness of the order of GPa. As a protein known for its amyloid-forming capacity, REF1 could be the main component responsible for GPa-range stiffness of NH3-stabilized films and might then play a crucial role on the final properties of natural rubber. |
format |
article |
topic_facet |
J11 - Manutention, transport, stockage et conservation des produits d'origine végétale F60 - Physiologie et biochimie végétale Hevea brasiliensis latex stockage caoutchouc propriété physicochimique protéine http://aims.fao.org/aos/agrovoc/c_3589 http://aims.fao.org/aos/agrovoc/c_4214 http://aims.fao.org/aos/agrovoc/c_7427 http://aims.fao.org/aos/agrovoc/c_6678 http://aims.fao.org/aos/agrovoc/c_1521 http://aims.fao.org/aos/agrovoc/c_6259 http://aims.fao.org/aos/agrovoc/c_7701 |
author |
Bottier, Céline Gross, Bryan Wadeesirisak, Kanthida Srisomboon, Surattiya Jantarasunthorn, Saowalak Musigamart, Natedao Roytrakul, Sittiruk Liengprayoon, Siriluck Vaysse, Laurent Kunemann, Philippe Vallat, Marie-France Mougin, Karine |
author_facet |
Bottier, Céline Gross, Bryan Wadeesirisak, Kanthida Srisomboon, Surattiya Jantarasunthorn, Saowalak Musigamart, Natedao Roytrakul, Sittiruk Liengprayoon, Siriluck Vaysse, Laurent Kunemann, Philippe Vallat, Marie-France Mougin, Karine |
author_sort |
Bottier, Céline |
title |
Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage |
title_short |
Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage |
title_full |
Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage |
title_fullStr |
Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage |
title_full_unstemmed |
Rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized Hevea latex during the first twelve days of storage |
title_sort |
rapid evolution of biochemical and physicochemical indicators of ammonia- stabilized hevea latex during the first twelve days of storage |
url |
http://agritrop.cirad.fr/591710/ http://agritrop.cirad.fr/591710/1/Coll%20Surf%20A_2019.pdf |
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