Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition
Cardiolipin (CL) is a phospholipid at the heart of mitochondrial metabolism, which plays a key role in mitochondrial function and bioenergetics. Among mitochondrial activity regulators, SIRT3 plays a crucial role in controlling the acetylation status of many enzymes participating in the energy metabolism in particular concerning lipid metabolism and fatty acid oxidation. Data suggest that possible connection may exist between SIRT3 and CL status that has not been evaluated in skeletal muscle. In the present study, we have characterized skeletal muscle lipids as well as mitochondrial lipids composition in mice overexpressing long (SIRT3-M1) and short (SIRT3-M3) isoforms of SIRT3. Particular attention has been paid for CL. We reported no alteration in muscle lipids content and fatty acids composition between the two mice SIRT3 strains and the control mice. However, mitochondrial CL content was significantly decreased in SIRT3-M3 mice and associated to an upregulation of tafazzin gene expression. In addition, mitochondrial phospholipids and fatty acids composition was altered with an increase in the PC/PE ratio and arachidonic acid content and a reduction in the MUFA/SFA ratio. These modifications in mitochondrial membrane composition are associated with a reduction in the enzymatic activities of mitochondrial respiratory chain complexes I and IV. In spite of these mitochondrial enzymatic alterations, skeletal muscle mitochondrial respiration remained similar in SIRT3-M3 and control mice. Surprisingly, none of those metabolic alterations were detected in mitochondria from SIRT3-M1 mice. In conclusion, our data indicate a specific action of the shorter SIRT3 isoform on lipid mitochondrial membrane biosynthesis and functioning.
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dig-cirad-fr-5876932021-11-09T09:38:33Z http://agritrop.cirad.fr/587693/ http://agritrop.cirad.fr/587693/ Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition. Chabi Béatrice, Fouret Gilles, Lecomte Jérôme, Cortade Fabienne, Pessemesse Laurence, Baati Narjes, Coudray Charles, Lin Ligen, Tong Qiang, Wrutniak-Cabello Chantal, Casas François, Feillet-Coudray Christine. 2018. Journal of Bioenergetics and Biomembranes, 50 (2) : 131-142.https://doi.org/10.1007/s10863-018-9752-1 <https://doi.org/10.1007/s10863-018-9752-1> Researchers Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition Chabi, Béatrice Fouret, Gilles Lecomte, Jérôme Cortade, Fabienne Pessemesse, Laurence Baati, Narjes Coudray, Charles Lin, Ligen Tong, Qiang Wrutniak-Cabello, Chantal Casas, François Feillet-Coudray, Christine eng 2018 Journal of Bioenergetics and Biomembranes Q02 - Traitement et conservation des produits alimentaires L50 - Physiologie et biochimie animales Cardiolipin (CL) is a phospholipid at the heart of mitochondrial metabolism, which plays a key role in mitochondrial function and bioenergetics. Among mitochondrial activity regulators, SIRT3 plays a crucial role in controlling the acetylation status of many enzymes participating in the energy metabolism in particular concerning lipid metabolism and fatty acid oxidation. Data suggest that possible connection may exist between SIRT3 and CL status that has not been evaluated in skeletal muscle. In the present study, we have characterized skeletal muscle lipids as well as mitochondrial lipids composition in mice overexpressing long (SIRT3-M1) and short (SIRT3-M3) isoforms of SIRT3. Particular attention has been paid for CL. We reported no alteration in muscle lipids content and fatty acids composition between the two mice SIRT3 strains and the control mice. However, mitochondrial CL content was significantly decreased in SIRT3-M3 mice and associated to an upregulation of tafazzin gene expression. In addition, mitochondrial phospholipids and fatty acids composition was altered with an increase in the PC/PE ratio and arachidonic acid content and a reduction in the MUFA/SFA ratio. These modifications in mitochondrial membrane composition are associated with a reduction in the enzymatic activities of mitochondrial respiratory chain complexes I and IV. In spite of these mitochondrial enzymatic alterations, skeletal muscle mitochondrial respiration remained similar in SIRT3-M3 and control mice. Surprisingly, none of those metabolic alterations were detected in mitochondria from SIRT3-M1 mice. In conclusion, our data indicate a specific action of the shorter SIRT3 isoform on lipid mitochondrial membrane biosynthesis and functioning. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/587693/1/s10863-018-9752-1.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s10863-018-9752-1 10.1007/s10863-018-9752-1 info:eu-repo/semantics/altIdentifier/doi/10.1007/s10863-018-9752-1 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s10863-018-9752-1 |
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Q02 - Traitement et conservation des produits alimentaires L50 - Physiologie et biochimie animales Q02 - Traitement et conservation des produits alimentaires L50 - Physiologie et biochimie animales |
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Q02 - Traitement et conservation des produits alimentaires L50 - Physiologie et biochimie animales Q02 - Traitement et conservation des produits alimentaires L50 - Physiologie et biochimie animales Chabi, Béatrice Fouret, Gilles Lecomte, Jérôme Cortade, Fabienne Pessemesse, Laurence Baati, Narjes Coudray, Charles Lin, Ligen Tong, Qiang Wrutniak-Cabello, Chantal Casas, François Feillet-Coudray, Christine Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
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Cardiolipin (CL) is a phospholipid at the heart of mitochondrial metabolism, which plays a key role in mitochondrial function and bioenergetics. Among mitochondrial activity regulators, SIRT3 plays a crucial role in controlling the acetylation status of many enzymes participating in the energy metabolism in particular concerning lipid metabolism and fatty acid oxidation. Data suggest that possible connection may exist between SIRT3 and CL status that has not been evaluated in skeletal muscle. In the present study, we have characterized skeletal muscle lipids as well as mitochondrial lipids composition in mice overexpressing long (SIRT3-M1) and short (SIRT3-M3) isoforms of SIRT3. Particular attention has been paid for CL. We reported no alteration in muscle lipids content and fatty acids composition between the two mice SIRT3 strains and the control mice. However, mitochondrial CL content was significantly decreased in SIRT3-M3 mice and associated to an upregulation of tafazzin gene expression. In addition, mitochondrial phospholipids and fatty acids composition was altered with an increase in the PC/PE ratio and arachidonic acid content and a reduction in the MUFA/SFA ratio. These modifications in mitochondrial membrane composition are associated with a reduction in the enzymatic activities of mitochondrial respiratory chain complexes I and IV. In spite of these mitochondrial enzymatic alterations, skeletal muscle mitochondrial respiration remained similar in SIRT3-M3 and control mice. Surprisingly, none of those metabolic alterations were detected in mitochondria from SIRT3-M1 mice. In conclusion, our data indicate a specific action of the shorter SIRT3 isoform on lipid mitochondrial membrane biosynthesis and functioning. |
format |
article |
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Q02 - Traitement et conservation des produits alimentaires L50 - Physiologie et biochimie animales |
author |
Chabi, Béatrice Fouret, Gilles Lecomte, Jérôme Cortade, Fabienne Pessemesse, Laurence Baati, Narjes Coudray, Charles Lin, Ligen Tong, Qiang Wrutniak-Cabello, Chantal Casas, François Feillet-Coudray, Christine |
author_facet |
Chabi, Béatrice Fouret, Gilles Lecomte, Jérôme Cortade, Fabienne Pessemesse, Laurence Baati, Narjes Coudray, Charles Lin, Ligen Tong, Qiang Wrutniak-Cabello, Chantal Casas, François Feillet-Coudray, Christine |
author_sort |
Chabi, Béatrice |
title |
Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
title_short |
Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
title_full |
Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
title_fullStr |
Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
title_full_unstemmed |
Skeletal muscle overexpression of short isoform Sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
title_sort |
skeletal muscle overexpression of short isoform sirt3 altered mitochondrial cardiolipin content and fatty acid composition |
url |
http://agritrop.cirad.fr/587693/ http://agritrop.cirad.fr/587693/1/s10863-018-9752-1.pdf |
work_keys_str_mv |
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