New rice resistance genes via targeted genome editing
Rice is one of the most important food crop in the world providing the principal source of calories for nearly half of its population. Bacterial Leaf Blight (BLB) of rice, caused by Xanthomonas oryzae pv. oryzae, depends on direct induction of host susceptibility genes by bacterial TAL (Transcription Activator-Like) effectors. So far, efficient control of this disease relies on the use of resistant varieties. Since bacterial ability to induce specific host susceptibility genes is critical for disease development, we wish to create mutations in rice susceptibility gene promoters using the TALEN (TAL Effectore Nuclease) technology, in order to make them unresponsive to bacterial infection. We show that (i) designer TALENs cleave DNA target sites in a predictable manner, (ii) engineered bacteria secrete TALENs into culture supernatants, and (iii) engineered TALEs injected into rice embryogenic tissues are able to induce their target genes efficiently. Based on the DNA recognition code, designer TALENs were created to induce site-specific protective mutations and thus mimic the natural evolution of resistance genes on a much faster scale. With this approach, we hope to provide breeders with new strategies to generate broad and durable resistance to bacterial infections, thus reducing chemical use, providing yield stability, and increasing profitability of rice farming, in particular for smallholder farmers. (Texte intégral)
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Subjects: | F30 - Génétique et amélioration des plantes, H20 - Maladies des plantes, Oryza sativa, résistance aux maladies, méthode de lutte, mutation provoquée, génie génétique, contrôle de maladies, Xanthomonas oryzae, gène, mécanisme de défense, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_5728, http://aims.fao.org/aos/agrovoc/c_3842, http://aims.fao.org/aos/agrovoc/c_15974, http://aims.fao.org/aos/agrovoc/c_2327, http://aims.fao.org/aos/agrovoc/c_24383, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_35269, |
Online Access: | http://agritrop.cirad.fr/587406/ http://agritrop.cirad.fr/587406/1/PHYTO-103-6-S2.1_New%20rice%20resistance.pdf |
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dig-cirad-fr-5874062024-01-29T00:54:56Z http://agritrop.cirad.fr/587406/ http://agritrop.cirad.fr/587406/ New rice resistance genes via targeted genome editing. Baufumé Servane, Meynard Donaldo, Reschke Maik, Solé Montserrat, Cunnac Sébastien, Szurek Boris, Guiderdoni Emmanuel, Boch Jen, Koebnik Ralf. 2013. Phytopathology, 103 (6), suppl. S2 : S2.13. APS-MSA Conference, Austin, États-Unis, 10 Août 2013/14 Août 2013.https://apsjournals.apsnet.org/doi/10.1094/PHYTO-103-6-S2.1 <https://apsjournals.apsnet.org/doi/10.1094/PHYTO-103-6-S2.1> New rice resistance genes via targeted genome editing Baufumé, Servane Meynard, Donaldo Reschke, Maik Solé, Montserrat Cunnac, Sébastien Szurek, Boris Guiderdoni, Emmanuel Boch, Jen Koebnik, Ralf eng 2013 Phytopathology F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa résistance aux maladies méthode de lutte mutation provoquée génie génétique contrôle de maladies Xanthomonas oryzae gène mécanisme de défense http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_5728 http://aims.fao.org/aos/agrovoc/c_3842 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_24383 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35269 Rice is one of the most important food crop in the world providing the principal source of calories for nearly half of its population. Bacterial Leaf Blight (BLB) of rice, caused by Xanthomonas oryzae pv. oryzae, depends on direct induction of host susceptibility genes by bacterial TAL (Transcription Activator-Like) effectors. So far, efficient control of this disease relies on the use of resistant varieties. Since bacterial ability to induce specific host susceptibility genes is critical for disease development, we wish to create mutations in rice susceptibility gene promoters using the TALEN (TAL Effectore Nuclease) technology, in order to make them unresponsive to bacterial infection. We show that (i) designer TALENs cleave DNA target sites in a predictable manner, (ii) engineered bacteria secrete TALENs into culture supernatants, and (iii) engineered TALEs injected into rice embryogenic tissues are able to induce their target genes efficiently. Based on the DNA recognition code, designer TALENs were created to induce site-specific protective mutations and thus mimic the natural evolution of resistance genes on a much faster scale. With this approach, we hope to provide breeders with new strategies to generate broad and durable resistance to bacterial infections, thus reducing chemical use, providing yield stability, and increasing profitability of rice farming, in particular for smallholder farmers. (Texte intégral) article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/587406/1/PHYTO-103-6-S2.1_New%20rice%20resistance.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://apsjournals.apsnet.org/doi/10.1094/PHYTO-103-6-S2.1 info:eu-repo/semantics/altIdentifier/purl/https://apsjournals.apsnet.org/doi/10.1094/PHYTO-103-6-S2.1 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa résistance aux maladies méthode de lutte mutation provoquée génie génétique contrôle de maladies Xanthomonas oryzae gène mécanisme de défense http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_5728 http://aims.fao.org/aos/agrovoc/c_3842 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_24383 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35269 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa résistance aux maladies méthode de lutte mutation provoquée génie génétique contrôle de maladies Xanthomonas oryzae gène mécanisme de défense http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_5728 http://aims.fao.org/aos/agrovoc/c_3842 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_24383 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35269 |
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F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa résistance aux maladies méthode de lutte mutation provoquée génie génétique contrôle de maladies Xanthomonas oryzae gène mécanisme de défense http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_5728 http://aims.fao.org/aos/agrovoc/c_3842 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_24383 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35269 F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa résistance aux maladies méthode de lutte mutation provoquée génie génétique contrôle de maladies Xanthomonas oryzae gène mécanisme de défense http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_5728 http://aims.fao.org/aos/agrovoc/c_3842 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_24383 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35269 Baufumé, Servane Meynard, Donaldo Reschke, Maik Solé, Montserrat Cunnac, Sébastien Szurek, Boris Guiderdoni, Emmanuel Boch, Jen Koebnik, Ralf New rice resistance genes via targeted genome editing |
description |
Rice is one of the most important food crop in the world providing the principal source of calories for nearly half of its population. Bacterial Leaf Blight (BLB) of rice, caused by Xanthomonas oryzae pv. oryzae, depends on direct induction of host susceptibility genes by bacterial TAL (Transcription Activator-Like) effectors. So far, efficient control of this disease relies on the use of resistant varieties. Since bacterial ability to induce specific host susceptibility genes is critical for disease development, we wish to create mutations in rice susceptibility gene promoters using the TALEN (TAL Effectore Nuclease) technology, in order to make them unresponsive to bacterial infection. We show that (i) designer TALENs cleave DNA target sites in a predictable manner, (ii) engineered bacteria secrete TALENs into culture supernatants, and (iii) engineered TALEs injected into rice embryogenic tissues are able to induce their target genes efficiently. Based on the DNA recognition code, designer TALENs were created to induce site-specific protective mutations and thus mimic the natural evolution of resistance genes on a much faster scale. With this approach, we hope to provide breeders with new strategies to generate broad and durable resistance to bacterial infections, thus reducing chemical use, providing yield stability, and increasing profitability of rice farming, in particular for smallholder farmers. (Texte intégral) |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes H20 - Maladies des plantes Oryza sativa résistance aux maladies méthode de lutte mutation provoquée génie génétique contrôle de maladies Xanthomonas oryzae gène mécanisme de défense http://aims.fao.org/aos/agrovoc/c_5438 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_5728 http://aims.fao.org/aos/agrovoc/c_3842 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_2327 http://aims.fao.org/aos/agrovoc/c_24383 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_35269 |
author |
Baufumé, Servane Meynard, Donaldo Reschke, Maik Solé, Montserrat Cunnac, Sébastien Szurek, Boris Guiderdoni, Emmanuel Boch, Jen Koebnik, Ralf |
author_facet |
Baufumé, Servane Meynard, Donaldo Reschke, Maik Solé, Montserrat Cunnac, Sébastien Szurek, Boris Guiderdoni, Emmanuel Boch, Jen Koebnik, Ralf |
author_sort |
Baufumé, Servane |
title |
New rice resistance genes via targeted genome editing |
title_short |
New rice resistance genes via targeted genome editing |
title_full |
New rice resistance genes via targeted genome editing |
title_fullStr |
New rice resistance genes via targeted genome editing |
title_full_unstemmed |
New rice resistance genes via targeted genome editing |
title_sort |
new rice resistance genes via targeted genome editing |
url |
http://agritrop.cirad.fr/587406/ http://agritrop.cirad.fr/587406/1/PHYTO-103-6-S2.1_New%20rice%20resistance.pdf |
work_keys_str_mv |
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1792499458579103744 |