Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers
We explored the Theobroma cacao genome sequence to progress in the knowledge of the T. cacao incompatibility system. Cocoa self-compatibility is an important yield factor and has been described as controlled by a late gameto-sporophytic system involving several locus, and resulting in gametic non-fusion. In this work, we identified two different mechanisms controlling the T. cacao self-incompatibility system at two separate loci, located on chromosome one and four (CH1 and CH4). Both loci are responsible for gametic selection, but only one (the CH4 locus) is involved in the main fruit drop. The CH1 locus acts prior to gamete fusion and independently of CH4 locus. Fine mapping and genome wide association studies focused analyses of restricted regions without recombinant plants where several candidate genes were identified. Their expression analysis showed differential expression during incompatible or compatible reactions for some of them. Highly polymorphic SSR diagnostic markers, designed in the CH4 region identified by fine mapping, allowed the development of efficient diagnostic markers predicting selfcompatibility and fruit setting according to allele or genotype presence. SSR alleles specific to self-compatible Amelonado and Criollo varieties were also identified allowing screening for self-compatible plants in cocoa populations.
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dig-cirad-fr-5866632022-03-31T10:13:38Z http://agritrop.cirad.fr/586663/ http://agritrop.cirad.fr/586663/ Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers. Lanaud Claire, Fouet Olivier, Legavre Thierry, Lopes Uilson Vanderlei, Sounigo Olivier, Eyango Marie Claire, Mermaz Benoit, da Silva Marcos Ramos, Loor Solorzano Rey Gaston, Argout Xavier, Gyapay Gabor, Ebaiarrey Herman Ebai, Colonges Kelly, Sanier Christine, Rivallan Ronan, Mastin Géraldine, Cryer Nicholas, Boccara Michel, Efombagn Mousseni Ives Bruno, Gramacho Karina Peres, Clément Didier. 2017. In : Proceedings of the first International Symposium on Cocoa Research ISCR 2017. ICCO. Lima : ICCO, 11 p. International Symposium on Cocoa Research – ISCR 2017 : Promoting Advances in Research to Enhance the Profitability of Cocoa Farming. 1, Lima, Pérou, 13 Novembre 2017/17 Novembre 2017.https://www.icco.org/icco-documentation/international-cocoa-symposium-2017/international-cocoa-symposium-2017-proceedings/#toggle-id-1 <https://www.icco.org/icco-documentation/international-cocoa-symposium-2017/international-cocoa-symposium-2017-proceedings/#toggle-id-1> Researchers Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers Lanaud, Claire Fouet, Olivier Legavre, Thierry Lopes, Uilson Vanderlei Sounigo, Olivier Eyango, Marie Claire Mermaz, Benoit da Silva, Marcos Ramos Loor Solorzano, Rey Gaston Argout, Xavier Gyapay, Gabor Ebaiarrey, Herman Ebai Colonges, Kelly Sanier, Christine Rivallan, Ronan Mastin, Géraldine Cryer, Nicholas Boccara, Michel Efombagn, Mousseni Ives Bruno Gramacho, Karina Peres Clément, Didier eng 2017 ICCO Proceedings of the first International Symposium on Cocoa Research ISCR 2017 F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction U30 - Méthodes de recherche We explored the Theobroma cacao genome sequence to progress in the knowledge of the T. cacao incompatibility system. Cocoa self-compatibility is an important yield factor and has been described as controlled by a late gameto-sporophytic system involving several locus, and resulting in gametic non-fusion. In this work, we identified two different mechanisms controlling the T. cacao self-incompatibility system at two separate loci, located on chromosome one and four (CH1 and CH4). Both loci are responsible for gametic selection, but only one (the CH4 locus) is involved in the main fruit drop. The CH1 locus acts prior to gamete fusion and independently of CH4 locus. Fine mapping and genome wide association studies focused analyses of restricted regions without recombinant plants where several candidate genes were identified. Their expression analysis showed differential expression during incompatible or compatible reactions for some of them. Highly polymorphic SSR diagnostic markers, designed in the CH4 region identified by fine mapping, allowed the development of efficient diagnostic markers predicting selfcompatibility and fruit setting according to allele or genotype presence. SSR alleles specific to self-compatible Amelonado and Criollo varieties were also identified allowing screening for self-compatible plants in cocoa populations. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586663/8/ID586663_Complet.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://www.icco.org/icco-documentation/international-cocoa-symposium-2017/international-cocoa-symposium-2017-proceedings/#toggle-id-1 info:eu-repo/semantics/altIdentifier/purl/https://www.icco.org/icco-documentation/international-cocoa-symposium-2017/international-cocoa-symposium-2017-proceedings/#toggle-id-1 |
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F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction U30 - Méthodes de recherche |
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F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction U30 - Méthodes de recherche F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction U30 - Méthodes de recherche Lanaud, Claire Fouet, Olivier Legavre, Thierry Lopes, Uilson Vanderlei Sounigo, Olivier Eyango, Marie Claire Mermaz, Benoit da Silva, Marcos Ramos Loor Solorzano, Rey Gaston Argout, Xavier Gyapay, Gabor Ebaiarrey, Herman Ebai Colonges, Kelly Sanier, Christine Rivallan, Ronan Mastin, Géraldine Cryer, Nicholas Boccara, Michel Efombagn, Mousseni Ives Bruno Gramacho, Karina Peres Clément, Didier Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers |
description |
We explored the Theobroma cacao genome sequence to progress in the knowledge of the T. cacao incompatibility system. Cocoa self-compatibility is an important yield factor and has been described as controlled by a late gameto-sporophytic system involving several locus, and resulting in gametic non-fusion. In this work, we identified two different mechanisms controlling the T. cacao self-incompatibility system at two separate loci, located on chromosome one and four (CH1 and CH4). Both loci are responsible for gametic selection, but only one (the CH4 locus) is involved in the main fruit drop. The CH1 locus acts prior to gamete fusion and independently of CH4 locus. Fine mapping and genome wide association studies focused analyses of restricted regions without recombinant plants where several candidate genes were identified. Their expression analysis showed differential expression during incompatible or compatible reactions for some of them. Highly polymorphic SSR diagnostic markers, designed in the CH4 region identified by fine mapping, allowed the development of efficient diagnostic markers predicting selfcompatibility and fruit setting according to allele or genotype presence. SSR alleles specific to self-compatible Amelonado and Criollo varieties were also identified allowing screening for self-compatible plants in cocoa populations. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes F63 - Physiologie végétale - Reproduction U30 - Méthodes de recherche |
author |
Lanaud, Claire Fouet, Olivier Legavre, Thierry Lopes, Uilson Vanderlei Sounigo, Olivier Eyango, Marie Claire Mermaz, Benoit da Silva, Marcos Ramos Loor Solorzano, Rey Gaston Argout, Xavier Gyapay, Gabor Ebaiarrey, Herman Ebai Colonges, Kelly Sanier, Christine Rivallan, Ronan Mastin, Géraldine Cryer, Nicholas Boccara, Michel Efombagn, Mousseni Ives Bruno Gramacho, Karina Peres Clément, Didier |
author_facet |
Lanaud, Claire Fouet, Olivier Legavre, Thierry Lopes, Uilson Vanderlei Sounigo, Olivier Eyango, Marie Claire Mermaz, Benoit da Silva, Marcos Ramos Loor Solorzano, Rey Gaston Argout, Xavier Gyapay, Gabor Ebaiarrey, Herman Ebai Colonges, Kelly Sanier, Christine Rivallan, Ronan Mastin, Géraldine Cryer, Nicholas Boccara, Michel Efombagn, Mousseni Ives Bruno Gramacho, Karina Peres Clément, Didier |
author_sort |
Lanaud, Claire |
title |
Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers |
title_short |
Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers |
title_full |
Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers |
title_fullStr |
Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers |
title_full_unstemmed |
Exploration of the T. cacao genome sequence to decipher the incompatibility system of Theobroma cacao and to identify diagnostic markers |
title_sort |
exploration of the t. cacao genome sequence to decipher the incompatibility system of theobroma cacao and to identify diagnostic markers |
publisher |
ICCO |
url |
http://agritrop.cirad.fr/586663/ http://agritrop.cirad.fr/586663/8/ID586663_Complet.pdf |
work_keys_str_mv |
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