Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity
The threat of bacterial resistance to antibiotics has created an urgent need to develop new antimicrobials. The aim of this study was to characterize the chemical diversity of Litsea cubeba leaf essential oil (EO) and its impacts on the antibacterial activity against pathogenic bacteria. Essential oils collected from seven provinces in North Vietnam (n = 25) were characterized by their high content in either 1,8‐cineole or linalool. Linalool‐type EOs were more effective against the eight bacterial strains tested than 1,8‐cineole‐type. Oil samples, LC19 (50% 1,8‐cineole) and BV27 (94% linalool), were selected to investigate their antibacterial mechanisms against Escherichia coli. A strong bactericidal effect was observed after 4 and 2 h of exposure respectively. Microscopic analysis of treated E. coli cultures clearly showed that EOs caused changes in cell morphology, loss of integrity and permeability of the cell membrane, as well as DNA loss. However, the effects of both EOs were distinct. LC19 mostly affected cell membrane, led to a significant cell filamentation rate and altered cell width, whereas BV27 damaged cell membrane integrity leading to cell permeabilization and altered nucleoid morphology with the appearance of spot and visibly altered compaction.
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Subjects: | F60 - Physiologie et biochimie végétale, 000 - Autres thèmes, huile essentielle, antimicrobien, feuille, http://aims.fao.org/aos/agrovoc/c_2669, http://aims.fao.org/aos/agrovoc/c_509, http://aims.fao.org/aos/agrovoc/c_4243, http://aims.fao.org/aos/agrovoc/c_8227, |
Online Access: | http://agritrop.cirad.fr/586590/ http://agritrop.cirad.fr/586590/7/Nguyen_et_al-2018-Letters_in_Applied_Microbiology.pdf |
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dig-cirad-fr-5865902024-01-29T00:44:16Z http://agritrop.cirad.fr/586590/ http://agritrop.cirad.fr/586590/ Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity. Nguyen Hai Van, Meile Jean-Christophe, Lebrun Marc, Caruso Domenico, Chu-Ky Son, Sarter Samira. 2018. Letters in Applied Microbiology, 66 (3) : 207-214.https://doi.org/10.1111/lam.12837 <https://doi.org/10.1111/lam.12837> Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity Nguyen, Hai Van Meile, Jean-Christophe Lebrun, Marc Caruso, Domenico Chu-Ky, Son Sarter, Samira eng 2018 Letters in Applied Microbiology F60 - Physiologie et biochimie végétale 000 - Autres thèmes huile essentielle antimicrobien feuille http://aims.fao.org/aos/agrovoc/c_2669 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_4243 Viet Nam http://aims.fao.org/aos/agrovoc/c_8227 The threat of bacterial resistance to antibiotics has created an urgent need to develop new antimicrobials. The aim of this study was to characterize the chemical diversity of Litsea cubeba leaf essential oil (EO) and its impacts on the antibacterial activity against pathogenic bacteria. Essential oils collected from seven provinces in North Vietnam (n = 25) were characterized by their high content in either 1,8‐cineole or linalool. Linalool‐type EOs were more effective against the eight bacterial strains tested than 1,8‐cineole‐type. Oil samples, LC19 (50% 1,8‐cineole) and BV27 (94% linalool), were selected to investigate their antibacterial mechanisms against Escherichia coli. A strong bactericidal effect was observed after 4 and 2 h of exposure respectively. Microscopic analysis of treated E. coli cultures clearly showed that EOs caused changes in cell morphology, loss of integrity and permeability of the cell membrane, as well as DNA loss. However, the effects of both EOs were distinct. LC19 mostly affected cell membrane, led to a significant cell filamentation rate and altered cell width, whereas BV27 damaged cell membrane integrity leading to cell permeabilization and altered nucleoid morphology with the appearance of spot and visibly altered compaction. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/586590/7/Nguyen_et_al-2018-Letters_in_Applied_Microbiology.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/lam.12837 10.1111/lam.12837 info:eu-repo/semantics/altIdentifier/doi/10.1111/lam.12837 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/lam.12837 |
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F60 - Physiologie et biochimie végétale 000 - Autres thèmes huile essentielle antimicrobien feuille http://aims.fao.org/aos/agrovoc/c_2669 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_8227 F60 - Physiologie et biochimie végétale 000 - Autres thèmes huile essentielle antimicrobien feuille http://aims.fao.org/aos/agrovoc/c_2669 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_8227 |
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F60 - Physiologie et biochimie végétale 000 - Autres thèmes huile essentielle antimicrobien feuille http://aims.fao.org/aos/agrovoc/c_2669 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_8227 F60 - Physiologie et biochimie végétale 000 - Autres thèmes huile essentielle antimicrobien feuille http://aims.fao.org/aos/agrovoc/c_2669 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_8227 Nguyen, Hai Van Meile, Jean-Christophe Lebrun, Marc Caruso, Domenico Chu-Ky, Son Sarter, Samira Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity |
description |
The threat of bacterial resistance to antibiotics has created an urgent need to develop new antimicrobials. The aim of this study was to characterize the chemical diversity of Litsea cubeba leaf essential oil (EO) and its impacts on the antibacterial activity against pathogenic bacteria. Essential oils collected from seven provinces in North Vietnam (n = 25) were characterized by their high content in either 1,8‐cineole or linalool. Linalool‐type EOs were more effective against the eight bacterial strains tested than 1,8‐cineole‐type. Oil samples, LC19 (50% 1,8‐cineole) and BV27 (94% linalool), were selected to investigate their antibacterial mechanisms against Escherichia coli. A strong bactericidal effect was observed after 4 and 2 h of exposure respectively. Microscopic analysis of treated E. coli cultures clearly showed that EOs caused changes in cell morphology, loss of integrity and permeability of the cell membrane, as well as DNA loss. However, the effects of both EOs were distinct. LC19 mostly affected cell membrane, led to a significant cell filamentation rate and altered cell width, whereas BV27 damaged cell membrane integrity leading to cell permeabilization and altered nucleoid morphology with the appearance of spot and visibly altered compaction. |
format |
article |
topic_facet |
F60 - Physiologie et biochimie végétale 000 - Autres thèmes huile essentielle antimicrobien feuille http://aims.fao.org/aos/agrovoc/c_2669 http://aims.fao.org/aos/agrovoc/c_509 http://aims.fao.org/aos/agrovoc/c_4243 http://aims.fao.org/aos/agrovoc/c_8227 |
author |
Nguyen, Hai Van Meile, Jean-Christophe Lebrun, Marc Caruso, Domenico Chu-Ky, Son Sarter, Samira |
author_facet |
Nguyen, Hai Van Meile, Jean-Christophe Lebrun, Marc Caruso, Domenico Chu-Ky, Son Sarter, Samira |
author_sort |
Nguyen, Hai Van |
title |
Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity |
title_short |
Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity |
title_full |
Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity |
title_fullStr |
Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity |
title_full_unstemmed |
Litsea cubeba leaf essential oil from Vietnam: chemical diversity and its impacts on antibacterial activity |
title_sort |
litsea cubeba leaf essential oil from vietnam: chemical diversity and its impacts on antibacterial activity |
url |
http://agritrop.cirad.fr/586590/ http://agritrop.cirad.fr/586590/7/Nguyen_et_al-2018-Letters_in_Applied_Microbiology.pdf |
work_keys_str_mv |
AT nguyenhaivan litseacubebaleafessentialoilfromvietnamchemicaldiversityanditsimpactsonantibacterialactivity AT meilejeanchristophe litseacubebaleafessentialoilfromvietnamchemicaldiversityanditsimpactsonantibacterialactivity AT lebrunmarc litseacubebaleafessentialoilfromvietnamchemicaldiversityanditsimpactsonantibacterialactivity AT carusodomenico litseacubebaleafessentialoilfromvietnamchemicaldiversityanditsimpactsonantibacterialactivity AT chukyson litseacubebaleafessentialoilfromvietnamchemicaldiversityanditsimpactsonantibacterialactivity AT sartersamira litseacubebaleafessentialoilfromvietnamchemicaldiversityanditsimpactsonantibacterialactivity |
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1792499407727362048 |