Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features
There is increasing interest in the heritable bacteria of invertebrate vectors of disease as they present novel targets for control initiatives. Previous studies on biting midges (Culicoides spp.), known to transmit several RNA viruses of veterinary importance, have revealed infections with the endosymbiotic bacteria, Wolbachia and Cardinium. However, rickettsial symbionts in these vectors are underexplored. Here, we present the genome of a previously uncharacterized Rickettsia endosymbiont from Culicoides newsteadi (RiCNE). This genome presents unique features potentially associated with host invasion and adaptation, including genes for the complete non-oxidative phase of the pentose phosphate pathway, and others predicted to mediate LPS and cell wall modification. Screening of 414 Culicoides individuals from 29 Palearctic or Afrotropical species revealed Rickettsia represent a widespread but previously overlooked association, reaching high frequencies in midge populations and present in 38% of the species tested. Sequence typing clusters the Rickettsia within the Torix group of the genus, a group known to infect several aquatic and hematophagous taxa. FISH analysis indicated the presence of Rickettsia bacteria in ovary tissue, indicating their maternal inheritance. Given the importance of biting midges as vectors, a key area of future research is to establish the impact of this endosymbiont on vector competence. This article is protected by copyright. All rights reserved.
id |
dig-cirad-fr-585234 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5852342024-01-29T00:28:53Z http://agritrop.cirad.fr/585234/ http://agritrop.cirad.fr/585234/ Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features. Pilgrim Jack, Ander Mats, Garros Claire, Baylis Matthew, Hurst Gregory D.D., Siozos Stefanos. 2017. Environmental Microbiology, 19 (10), n.spéc. Pathogen and Drug Resistance Ecology : 4238-4255.https://doi.org/10.1111/1462-2920.13887 <https://doi.org/10.1111/1462-2920.13887> Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features Pilgrim, Jack Ander, Mats Garros, Claire Baylis, Matthew Hurst, Gregory D.D. Siozos, Stefanos eng 2017 Environmental Microbiology L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Rickettsia Culicoides symbiote génome flore bactérienne infection symbiose vecteur de maladie transmission des maladies interactions biologiques relation hôte parasite Cecidomyiidae http://aims.fao.org/aos/agrovoc/c_13925 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_1402 There is increasing interest in the heritable bacteria of invertebrate vectors of disease as they present novel targets for control initiatives. Previous studies on biting midges (Culicoides spp.), known to transmit several RNA viruses of veterinary importance, have revealed infections with the endosymbiotic bacteria, Wolbachia and Cardinium. However, rickettsial symbionts in these vectors are underexplored. Here, we present the genome of a previously uncharacterized Rickettsia endosymbiont from Culicoides newsteadi (RiCNE). This genome presents unique features potentially associated with host invasion and adaptation, including genes for the complete non-oxidative phase of the pentose phosphate pathway, and others predicted to mediate LPS and cell wall modification. Screening of 414 Culicoides individuals from 29 Palearctic or Afrotropical species revealed Rickettsia represent a widespread but previously overlooked association, reaching high frequencies in midge populations and present in 38% of the species tested. Sequence typing clusters the Rickettsia within the Torix group of the genus, a group known to infect several aquatic and hematophagous taxa. FISH analysis indicated the presence of Rickettsia bacteria in ovary tissue, indicating their maternal inheritance. Given the importance of biting midges as vectors, a key area of future research is to establish the impact of this endosymbiont on vector competence. This article is protected by copyright. All rights reserved. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/585234/7/Pilgrim_et_al-4238-Environmental_Microbiology.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/1462-2920.13887 10.1111/1462-2920.13887 info:eu-repo/semantics/altIdentifier/doi/10.1111/1462-2920.13887 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/1462-2920.13887 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Rickettsia Culicoides symbiote génome flore bactérienne infection symbiose vecteur de maladie transmission des maladies interactions biologiques relation hôte parasite Cecidomyiidae http://aims.fao.org/aos/agrovoc/c_13925 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_1402 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Rickettsia Culicoides symbiote génome flore bactérienne infection symbiose vecteur de maladie transmission des maladies interactions biologiques relation hôte parasite Cecidomyiidae http://aims.fao.org/aos/agrovoc/c_13925 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_1402 |
spellingShingle |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Rickettsia Culicoides symbiote génome flore bactérienne infection symbiose vecteur de maladie transmission des maladies interactions biologiques relation hôte parasite Cecidomyiidae http://aims.fao.org/aos/agrovoc/c_13925 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_1402 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Rickettsia Culicoides symbiote génome flore bactérienne infection symbiose vecteur de maladie transmission des maladies interactions biologiques relation hôte parasite Cecidomyiidae http://aims.fao.org/aos/agrovoc/c_13925 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_1402 Pilgrim, Jack Ander, Mats Garros, Claire Baylis, Matthew Hurst, Gregory D.D. Siozos, Stefanos Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features |
description |
There is increasing interest in the heritable bacteria of invertebrate vectors of disease as they present novel targets for control initiatives. Previous studies on biting midges (Culicoides spp.), known to transmit several RNA viruses of veterinary importance, have revealed infections with the endosymbiotic bacteria, Wolbachia and Cardinium. However, rickettsial symbionts in these vectors are underexplored. Here, we present the genome of a previously uncharacterized Rickettsia endosymbiont from Culicoides newsteadi (RiCNE). This genome presents unique features potentially associated with host invasion and adaptation, including genes for the complete non-oxidative phase of the pentose phosphate pathway, and others predicted to mediate LPS and cell wall modification. Screening of 414 Culicoides individuals from 29 Palearctic or Afrotropical species revealed Rickettsia represent a widespread but previously overlooked association, reaching high frequencies in midge populations and present in 38% of the species tested. Sequence typing clusters the Rickettsia within the Torix group of the genus, a group known to infect several aquatic and hematophagous taxa. FISH analysis indicated the presence of Rickettsia bacteria in ovary tissue, indicating their maternal inheritance. Given the importance of biting midges as vectors, a key area of future research is to establish the impact of this endosymbiont on vector competence. This article is protected by copyright. All rights reserved. |
format |
article |
topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux Rickettsia Culicoides symbiote génome flore bactérienne infection symbiose vecteur de maladie transmission des maladies interactions biologiques relation hôte parasite Cecidomyiidae http://aims.fao.org/aos/agrovoc/c_13925 http://aims.fao.org/aos/agrovoc/c_10196 http://aims.fao.org/aos/agrovoc/c_7562 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_10916 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_49896 http://aims.fao.org/aos/agrovoc/c_11620 http://aims.fao.org/aos/agrovoc/c_1402 |
author |
Pilgrim, Jack Ander, Mats Garros, Claire Baylis, Matthew Hurst, Gregory D.D. Siozos, Stefanos |
author_facet |
Pilgrim, Jack Ander, Mats Garros, Claire Baylis, Matthew Hurst, Gregory D.D. Siozos, Stefanos |
author_sort |
Pilgrim, Jack |
title |
Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features |
title_short |
Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features |
title_full |
Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features |
title_fullStr |
Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features |
title_full_unstemmed |
Torix group Rickettsia are widespread in Culicoides biting midges (Diptera: Ceratopogonidae), reach high frequency and carry unique genomic features |
title_sort |
torix group rickettsia are widespread in culicoides biting midges (diptera: ceratopogonidae), reach high frequency and carry unique genomic features |
url |
http://agritrop.cirad.fr/585234/ http://agritrop.cirad.fr/585234/7/Pilgrim_et_al-4238-Environmental_Microbiology.pdf |
work_keys_str_mv |
AT pilgrimjack torixgrouprickettsiaarewidespreadinculicoidesbitingmidgesdipteraceratopogonidaereachhighfrequencyandcarryuniquegenomicfeatures AT andermats torixgrouprickettsiaarewidespreadinculicoidesbitingmidgesdipteraceratopogonidaereachhighfrequencyandcarryuniquegenomicfeatures AT garrosclaire torixgrouprickettsiaarewidespreadinculicoidesbitingmidgesdipteraceratopogonidaereachhighfrequencyandcarryuniquegenomicfeatures AT baylismatthew torixgrouprickettsiaarewidespreadinculicoidesbitingmidgesdipteraceratopogonidaereachhighfrequencyandcarryuniquegenomicfeatures AT hurstgregorydd torixgrouprickettsiaarewidespreadinculicoidesbitingmidgesdipteraceratopogonidaereachhighfrequencyandcarryuniquegenomicfeatures AT siozosstefanos torixgrouprickettsiaarewidespreadinculicoidesbitingmidgesdipteraceratopogonidaereachhighfrequencyandcarryuniquegenomicfeatures |
_version_ |
1792499331308191744 |