Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils
Insecticidal Cry, or Bt, proteins are produced by the soil-endemic bacterium, Bacillus thuringiensis and some genetically modified crops. Their environmental fate depends on interactions with soil. Little is known about the toxicity of adsorbed proteins and the change in toxicity over time. We incubated Cry1Ac and Cry2A in contrasting soils subjected to different treatments to inhibit microbial activity. The toxin was chemically extracted and immunoassayed. Manduca sexta was the target insect for biotests. Extractable toxin decreased during incubation for up to four weeks. Toxicity of Cry1Ac was maintained in the adsorbed state, but lost after 2 weeks incubation at 25 °C. The decline in extractable protein and toxicity were much slower at 4 °C with no significant effect of soil sterilization. The major driving force for decline may be time-dependent fixation of adsorbed protein, leading to a decrease in the extraction yield in vitro, paralleled by decreasing solubilisation in the larval gut.
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Subjects: | H02 - Pesticides, P02 - Pollution, P33 - Chimie et physique du sol, Bacillus thuringiensis, biopesticide, persistance des pesticides, sol, adsorption, toxicité du sol, pollution du sol, pollution par l'agriculture, protéine bactérienne, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_27467, http://aims.fao.org/aos/agrovoc/c_5735, http://aims.fao.org/aos/agrovoc/c_7156, http://aims.fao.org/aos/agrovoc/c_137, http://aims.fao.org/aos/agrovoc/c_7201, http://aims.fao.org/aos/agrovoc/c_7183, http://aims.fao.org/aos/agrovoc/c_28744, http://aims.fao.org/aos/agrovoc/c_24141, http://aims.fao.org/aos/agrovoc/c_4188, http://aims.fao.org/aos/agrovoc/c_3081, |
Online Access: | http://agritrop.cirad.fr/580066/ http://agritrop.cirad.fr/580066/1/580066.pdf |
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dig-cirad-fr-5800662024-01-28T23:24:12Z http://agritrop.cirad.fr/580066/ http://agritrop.cirad.fr/580066/ Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils. Hung Truong Phuc, Truong Le Van, Binh Ngo Dinh, Frutos Roger, Quiquampoix Hervé, Staunton Siobhán. 2016. Environmental Pollution, 208 (Part B) : 318-325.https://doi.org/10.1016/j.envpol.2015.09.046 <https://doi.org/10.1016/j.envpol.2015.09.046> Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán eng 2016 Environmental Pollution H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide persistance des pesticides sol adsorption toxicité du sol pollution du sol pollution par l'agriculture protéine bactérienne http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7156 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_7201 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_24141 Languedoc-Roussillon France http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 Insecticidal Cry, or Bt, proteins are produced by the soil-endemic bacterium, Bacillus thuringiensis and some genetically modified crops. Their environmental fate depends on interactions with soil. Little is known about the toxicity of adsorbed proteins and the change in toxicity over time. We incubated Cry1Ac and Cry2A in contrasting soils subjected to different treatments to inhibit microbial activity. The toxin was chemically extracted and immunoassayed. Manduca sexta was the target insect for biotests. Extractable toxin decreased during incubation for up to four weeks. Toxicity of Cry1Ac was maintained in the adsorbed state, but lost after 2 weeks incubation at 25 °C. The decline in extractable protein and toxicity were much slower at 4 °C with no significant effect of soil sterilization. The major driving force for decline may be time-dependent fixation of adsorbed protein, leading to a decrease in the extraction yield in vitro, paralleled by decreasing solubilisation in the larval gut. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/580066/1/580066.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.envpol.2015.09.046 10.1016/j.envpol.2015.09.046 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2015.09.046 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.envpol.2015.09.046 |
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H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide persistance des pesticides sol adsorption toxicité du sol pollution du sol pollution par l'agriculture protéine bactérienne http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7156 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_7201 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide persistance des pesticides sol adsorption toxicité du sol pollution du sol pollution par l'agriculture protéine bactérienne http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7156 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_7201 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 |
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H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide persistance des pesticides sol adsorption toxicité du sol pollution du sol pollution par l'agriculture protéine bactérienne http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7156 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_7201 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide persistance des pesticides sol adsorption toxicité du sol pollution du sol pollution par l'agriculture protéine bactérienne http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7156 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_7201 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils |
description |
Insecticidal Cry, or Bt, proteins are produced by the soil-endemic bacterium, Bacillus thuringiensis and some genetically modified crops. Their environmental fate depends on interactions with soil. Little is known about the toxicity of adsorbed proteins and the change in toxicity over time. We incubated Cry1Ac and Cry2A in contrasting soils subjected to different treatments to inhibit microbial activity. The toxin was chemically extracted and immunoassayed. Manduca sexta was the target insect for biotests. Extractable toxin decreased during incubation for up to four weeks. Toxicity of Cry1Ac was maintained in the adsorbed state, but lost after 2 weeks incubation at 25 °C. The decline in extractable protein and toxicity were much slower at 4 °C with no significant effect of soil sterilization. The major driving force for decline may be time-dependent fixation of adsorbed protein, leading to a decrease in the extraction yield in vitro, paralleled by decreasing solubilisation in the larval gut. |
format |
article |
topic_facet |
H02 - Pesticides P02 - Pollution P33 - Chimie et physique du sol Bacillus thuringiensis biopesticide persistance des pesticides sol adsorption toxicité du sol pollution du sol pollution par l'agriculture protéine bactérienne http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27467 http://aims.fao.org/aos/agrovoc/c_5735 http://aims.fao.org/aos/agrovoc/c_7156 http://aims.fao.org/aos/agrovoc/c_137 http://aims.fao.org/aos/agrovoc/c_7201 http://aims.fao.org/aos/agrovoc/c_7183 http://aims.fao.org/aos/agrovoc/c_28744 http://aims.fao.org/aos/agrovoc/c_24141 http://aims.fao.org/aos/agrovoc/c_4188 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán |
author_facet |
Hung, Truong Phuc Truong, Le Van Binh, Ngo Dinh Frutos, Roger Quiquampoix, Hervé Staunton, Siobhán |
author_sort |
Hung, Truong Phuc |
title |
Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils |
title_short |
Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils |
title_full |
Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils |
title_fullStr |
Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils |
title_full_unstemmed |
Persistence of detectable insecticidal proteins from Bacillus thuringiensis (Cry) and toxicity after adsorption on contrasting soils |
title_sort |
persistence of detectable insecticidal proteins from bacillus thuringiensis (cry) and toxicity after adsorption on contrasting soils |
url |
http://agritrop.cirad.fr/580066/ http://agritrop.cirad.fr/580066/1/580066.pdf |
work_keys_str_mv |
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_version_ |
1792499027573473280 |