The albicidin resistance factor AlbD is a serine endopeptidase that hydrolyzes unusual oligoaromatic-type peptides

The para-aminobenzoic acid-containing peptide albicidin is a pathogenicity factor synthesized by Xanthomonas albilineans in infections of sugar cane. Albicidin is a nanomolar inhibitor of the bacterial DNA gyrase with a strong activity against various Gram-negative bacteria. The bacterium Pantoea dispersa expresses the hydrolase AlbD, conferring natural resistance against albicidin. We show that AlbD is a novel type of endopeptidase that catalyzes the cleavage of albicidin at a peptide backbone amide bond, thus abolishing its antimicrobial activity. Additionally, we determined the minimal cleavage motif of AlbD with substrates derived by chemical synthesis. Our results clearly identify AlbD as a unique endopeptidase that is the first member of a new subfamily of peptidases. Our findings provide the molecular basis for a natural detoxification mechanism, potentially rendering a new tool in biological chemistry approaches.

Bibliographic Details

Main Authors:	Vieweg, Laura, Kretz, Julian, Pesic, Alexander, Kerwat, Dennis, Grätz, Stefan, Royer, Monique, Cociancich, Stéphane, Mainz, Andi, Süssmuth, Roderich
Format:	article biblioteca
Language:	eng
Subjects:	000 - Autres thèmes, H20 - Maladies des plantes, Xanthomonas albilineans, isomérase, peptide, composé aromatique, antimicrobien, acide benzoïque, peptidase, inhibiteur d'enzyme, propriété antimicrobienne, bactérie gram négatif, détoxification métabolique, activité enzymatique, Saccharum officinarum, biologie moléculaire, http://aims.fao.org/aos/agrovoc/c_27422, http://aims.fao.org/aos/agrovoc/c_3967, http://aims.fao.org/aos/agrovoc/c_5691, http://aims.fao.org/aos/agrovoc/c_622, http://aims.fao.org/aos/agrovoc/c_509, http://aims.fao.org/aos/agrovoc/c_881, http://aims.fao.org/aos/agrovoc/c_5690, http://aims.fao.org/aos/agrovoc/c_2599, http://aims.fao.org/aos/agrovoc/c_26756, http://aims.fao.org/aos/agrovoc/c_3352, http://aims.fao.org/aos/agrovoc/c_34050, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_6727, http://aims.fao.org/aos/agrovoc/c_4891,
Online Access:	http://agritrop.cirad.fr/577983/ http://agritrop.cirad.fr/577983/1/2015_JACS_Vieweg_AlbD.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!