Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants
This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species.
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| Subjects: | H20 - Maladies des plantes, U30 - Méthodes de recherche, |
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dig-cirad-fr-5760562022-04-15T09:42:58Z http://agritrop.cirad.fr/576056/ http://agritrop.cirad.fr/576056/ Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants. Filloux Denis, Dallot Sylvie, Delaunay Agnès, Galzi Serge, Jacquot Emmanuel, Roumagnac Philippe. 2015. In : Plant pathology: techniques and protocols. Lacomme Christophe (ed.). New York : Springer [Etats-Unis], 249-257. (Methods in Molecular Biology, 1302) ISBN 978-1-4939-2619-0https://doi.org/10.1007/978-1-4939-2620-6_18 <https://doi.org/10.1007/978-1-4939-2620-6_18> Researchers Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants Filloux, Denis Dallot, Sylvie Delaunay, Agnès Galzi, Serge Jacquot, Emmanuel Roumagnac, Philippe eng 2015 Springer [Etats-Unis] Plant pathology: techniques and protocols H20 - Maladies des plantes U30 - Méthodes de recherche This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species. book_section info:eu-repo/semantics/bookPart Chapter info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/576056/1/ID%20576056.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/978-1-4939-2620-6_18 10.1007/978-1-4939-2620-6_18 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=218301 info:eu-repo/semantics/altIdentifier/doi/10.1007/978-1-4939-2620-6_18 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/978-1-4939-2620-6_18 |
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H20 - Maladies des plantes U30 - Méthodes de recherche H20 - Maladies des plantes U30 - Méthodes de recherche |
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H20 - Maladies des plantes U30 - Méthodes de recherche H20 - Maladies des plantes U30 - Méthodes de recherche Filloux, Denis Dallot, Sylvie Delaunay, Agnès Galzi, Serge Jacquot, Emmanuel Roumagnac, Philippe Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants |
| description |
This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species. |
| format |
book_section |
| topic_facet |
H20 - Maladies des plantes U30 - Méthodes de recherche |
| author |
Filloux, Denis Dallot, Sylvie Delaunay, Agnès Galzi, Serge Jacquot, Emmanuel Roumagnac, Philippe |
| author_facet |
Filloux, Denis Dallot, Sylvie Delaunay, Agnès Galzi, Serge Jacquot, Emmanuel Roumagnac, Philippe |
| author_sort |
Filloux, Denis |
| title |
Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants |
| title_short |
Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants |
| title_full |
Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants |
| title_fullStr |
Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants |
| title_full_unstemmed |
Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants |
| title_sort |
metagenomics approaches based on virion-associated nucleic acids (vana): an innovative tool for assessing without a priori viral diversity of plants |
| publisher |
Springer [Etats-Unis] |
| url |
http://agritrop.cirad.fr/576056/ http://agritrop.cirad.fr/576056/1/ID%20576056.pdf |
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1758024510760550400 |