An update on Awka wilt disease of coconut caused by Phytoplasma In Nigeria : P14.004
Lethal yellowing disease (LYD) caused by phytoplasma is the most damaging threat to coconut in Africa. Nigeria is the first African country where the disease (Awka wilt) was reported in 1917. The symptoms of the disease are similar to those reported in other West and East African countries. In West Africa, LYD is caused by a phytoplasma belonging to the 16SrXXII group, and the disease is still very active in Nigeria and Ghana. In early 1990s, sequencing of the 16SrDNA of one Ghanaian and one Nigerian isolate confirmed the implication of a very similar but not identical phytoplasma strains in the two countries. In 2012, a new survey for coconut LYD covering most of the coastal Nigerian States was done. Symptomatic coconut trees were observed in all the States surveyed. Stem samples were collected from symptomatic trees from three Eastern States around Awka. From each sample, DNA was extracted and the 16SrDNA was amplified by PCR using P1/P7 primers. The PCR products were sequenced and compared with each other and with the LDN sequence published 20 years ago. A homology of 100% was observed between the 16SrDNA sequence previously published and the sequences of our samples. Based on the known conserved 16SrDNA sequences, this result suggests a relative stability of the LYD phytoplasma populations in Eastern Region of Nigeria and absence of introduction of the "Ghanaian strain". A similar investigation for symptomatic coconut from the Western Region and by using more variable genes is necessary.
| Main Authors: | , , |
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| Format: | conference_item biblioteca |
| Language: | eng |
| Published: |
Chinese Society for Plant Pathology
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| Subjects: | H20 - Maladies des plantes, |
| Online Access: | http://agritrop.cirad.fr/571586/ http://agritrop.cirad.fr/571586/1/document_571586.pdf |
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