Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition
Oil palm (Elaeis guineensis) produces two oils of major economic importance, commonly referred to as palm oil and palm kernel oil, extracted from the mesocarp and the endosperm, respectively. While lauric acid predominates in endosperm oil, the major fatty acids (FAs) of mesocarp oil are palmitic and oleic acids. The oil palm embryo also stores oil, which contains a significant proportion of linoleic acid. In addition, the three tissues display high variation for oil content at maturity. To gain insight into the mechanisms that govern such differences in oil content and FA composition, tissue transcriptome and lipid composition were compared during development. The contribution of the cytosolic and plastidial glycolytic routes differed markedly between the mesocarp and seed tissues, but transcriptional patterns of genes involved in the conversion of sucrose to pyruvate were not related to variations for oil content. Accumulation of lauric acid relied on the dramatic up-regulation of a specialized acyl-acyl carrier protein thioesterase paralog and the concerted recruitment of specific isoforms of triacylglycerol assembly enzymes. Three paralogs of the WRINKLED1 (WRI1) transcription factor were identified, of which EgWRI1-1 and EgWRI1-2 were massively transcribed during oil deposition in the mesocarp and the endosperm, respectively. None of the three WRI1 paralogs were detected in the embryo. The transcription level of FA synthesis genes correlated with the amount of WRI1 transcripts and oil content. Changes in triacylglycerol content and FA composition of Nicotiana benthamiana leaves infiltrated with various combinations of WRI1 and FatB paralogs from oil palm validated functions inferred from transcriptome analysis.
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dig-cirad-fr-5697632024-01-28T21:31:18Z http://agritrop.cirad.fr/569763/ http://agritrop.cirad.fr/569763/ Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition. Dussert Stéphane, Guérin Chloé, Andersson Mariette, Joët Thierry, Tranbarger Timothy John, Pizot Maxime, Gautier Sarah, Omoré Alphonse, Durand-Gasselin Tristan, Morcillo Fabienne. 2013. Plant Physiology, 162 (3) : 1337-1358.https://doi.org/10.1104/pp.113.220525 <https://doi.org/10.1104/pp.113.220525> Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition Dussert, Stéphane Guérin, Chloé Andersson, Mariette Joët, Thierry Tranbarger, Timothy John Pizot, Maxime Gautier, Sarah Omoré, Alphonse Durand-Gasselin, Tristan Morcillo, Fabienne eng 2013 Plant Physiology F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes Q04 - Composition des produits alimentaires Elaeis guineensis composition chimique teneur en lipides acide gras acide laurique acide oléique acide linoléique biosynthèse physiologie végétale transcription génétique graine oléagineuse fruit (botanique) huile de palmiste huile de palme http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_4360 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_4226 http://aims.fao.org/aos/agrovoc/c_5341 http://aims.fao.org/aos/agrovoc/c_4352 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_25490 http://aims.fao.org/aos/agrovoc/c_3119 http://aims.fao.org/aos/agrovoc/c_5515 http://aims.fao.org/aos/agrovoc/c_5514 Bénin http://aims.fao.org/aos/agrovoc/c_875 Oil palm (Elaeis guineensis) produces two oils of major economic importance, commonly referred to as palm oil and palm kernel oil, extracted from the mesocarp and the endosperm, respectively. While lauric acid predominates in endosperm oil, the major fatty acids (FAs) of mesocarp oil are palmitic and oleic acids. The oil palm embryo also stores oil, which contains a significant proportion of linoleic acid. In addition, the three tissues display high variation for oil content at maturity. To gain insight into the mechanisms that govern such differences in oil content and FA composition, tissue transcriptome and lipid composition were compared during development. The contribution of the cytosolic and plastidial glycolytic routes differed markedly between the mesocarp and seed tissues, but transcriptional patterns of genes involved in the conversion of sucrose to pyruvate were not related to variations for oil content. Accumulation of lauric acid relied on the dramatic up-regulation of a specialized acyl-acyl carrier protein thioesterase paralog and the concerted recruitment of specific isoforms of triacylglycerol assembly enzymes. Three paralogs of the WRINKLED1 (WRI1) transcription factor were identified, of which EgWRI1-1 and EgWRI1-2 were massively transcribed during oil deposition in the mesocarp and the endosperm, respectively. None of the three WRI1 paralogs were detected in the embryo. The transcription level of FA synthesis genes correlated with the amount of WRI1 transcripts and oil content. Changes in triacylglycerol content and FA composition of Nicotiana benthamiana leaves infiltrated with various combinations of WRI1 and FatB paralogs from oil palm validated functions inferred from transcriptome analysis. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/569763/1/document_569763.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1104/pp.113.220525 10.1104/pp.113.220525 info:eu-repo/semantics/altIdentifier/doi/10.1104/pp.113.220525 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1104/pp.113.220525 |
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F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes Q04 - Composition des produits alimentaires Elaeis guineensis composition chimique teneur en lipides acide gras acide laurique acide oléique acide linoléique biosynthèse physiologie végétale transcription génétique graine oléagineuse fruit (botanique) huile de palmiste huile de palme http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_4360 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_4226 http://aims.fao.org/aos/agrovoc/c_5341 http://aims.fao.org/aos/agrovoc/c_4352 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_25490 http://aims.fao.org/aos/agrovoc/c_3119 http://aims.fao.org/aos/agrovoc/c_5515 http://aims.fao.org/aos/agrovoc/c_5514 http://aims.fao.org/aos/agrovoc/c_875 F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes Q04 - Composition des produits alimentaires Elaeis guineensis composition chimique teneur en lipides acide gras acide laurique acide oléique acide linoléique biosynthèse physiologie végétale transcription génétique graine oléagineuse fruit (botanique) huile de palmiste huile de palme http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_4360 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_4226 http://aims.fao.org/aos/agrovoc/c_5341 http://aims.fao.org/aos/agrovoc/c_4352 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_25490 http://aims.fao.org/aos/agrovoc/c_3119 http://aims.fao.org/aos/agrovoc/c_5515 http://aims.fao.org/aos/agrovoc/c_5514 http://aims.fao.org/aos/agrovoc/c_875 |
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F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes Q04 - Composition des produits alimentaires Elaeis guineensis composition chimique teneur en lipides acide gras acide laurique acide oléique acide linoléique biosynthèse physiologie végétale transcription génétique graine oléagineuse fruit (botanique) huile de palmiste huile de palme http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_4360 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_4226 http://aims.fao.org/aos/agrovoc/c_5341 http://aims.fao.org/aos/agrovoc/c_4352 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_25490 http://aims.fao.org/aos/agrovoc/c_3119 http://aims.fao.org/aos/agrovoc/c_5515 http://aims.fao.org/aos/agrovoc/c_5514 http://aims.fao.org/aos/agrovoc/c_875 F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes Q04 - Composition des produits alimentaires Elaeis guineensis composition chimique teneur en lipides acide gras acide laurique acide oléique acide linoléique biosynthèse physiologie végétale transcription génétique graine oléagineuse fruit (botanique) huile de palmiste huile de palme http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_4360 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_4226 http://aims.fao.org/aos/agrovoc/c_5341 http://aims.fao.org/aos/agrovoc/c_4352 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_25490 http://aims.fao.org/aos/agrovoc/c_3119 http://aims.fao.org/aos/agrovoc/c_5515 http://aims.fao.org/aos/agrovoc/c_5514 http://aims.fao.org/aos/agrovoc/c_875 Dussert, Stéphane Guérin, Chloé Andersson, Mariette Joët, Thierry Tranbarger, Timothy John Pizot, Maxime Gautier, Sarah Omoré, Alphonse Durand-Gasselin, Tristan Morcillo, Fabienne Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
description |
Oil palm (Elaeis guineensis) produces two oils of major economic importance, commonly referred to as palm oil and palm kernel oil, extracted from the mesocarp and the endosperm, respectively. While lauric acid predominates in endosperm oil, the major fatty acids (FAs) of mesocarp oil are palmitic and oleic acids. The oil palm embryo also stores oil, which contains a significant proportion of linoleic acid. In addition, the three tissues display high variation for oil content at maturity. To gain insight into the mechanisms that govern such differences in oil content and FA composition, tissue transcriptome and lipid composition were compared during development. The contribution of the cytosolic and plastidial glycolytic routes differed markedly between the mesocarp and seed tissues, but transcriptional patterns of genes involved in the conversion of sucrose to pyruvate were not related to variations for oil content. Accumulation of lauric acid relied on the dramatic up-regulation of a specialized acyl-acyl carrier protein thioesterase paralog and the concerted recruitment of specific isoforms of triacylglycerol assembly enzymes. Three paralogs of the WRINKLED1 (WRI1) transcription factor were identified, of which EgWRI1-1 and EgWRI1-2 were massively transcribed during oil deposition in the mesocarp and the endosperm, respectively. None of the three WRI1 paralogs were detected in the embryo. The transcription level of FA synthesis genes correlated with the amount of WRI1 transcripts and oil content. Changes in triacylglycerol content and FA composition of Nicotiana benthamiana leaves infiltrated with various combinations of WRI1 and FatB paralogs from oil palm validated functions inferred from transcriptome analysis. |
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article |
topic_facet |
F60 - Physiologie et biochimie végétale F30 - Génétique et amélioration des plantes Q04 - Composition des produits alimentaires Elaeis guineensis composition chimique teneur en lipides acide gras acide laurique acide oléique acide linoléique biosynthèse physiologie végétale transcription génétique graine oléagineuse fruit (botanique) huile de palmiste huile de palme http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_4360 http://aims.fao.org/aos/agrovoc/c_2818 http://aims.fao.org/aos/agrovoc/c_4226 http://aims.fao.org/aos/agrovoc/c_5341 http://aims.fao.org/aos/agrovoc/c_4352 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_3222 http://aims.fao.org/aos/agrovoc/c_25490 http://aims.fao.org/aos/agrovoc/c_3119 http://aims.fao.org/aos/agrovoc/c_5515 http://aims.fao.org/aos/agrovoc/c_5514 http://aims.fao.org/aos/agrovoc/c_875 |
author |
Dussert, Stéphane Guérin, Chloé Andersson, Mariette Joët, Thierry Tranbarger, Timothy John Pizot, Maxime Gautier, Sarah Omoré, Alphonse Durand-Gasselin, Tristan Morcillo, Fabienne |
author_facet |
Dussert, Stéphane Guérin, Chloé Andersson, Mariette Joët, Thierry Tranbarger, Timothy John Pizot, Maxime Gautier, Sarah Omoré, Alphonse Durand-Gasselin, Tristan Morcillo, Fabienne |
author_sort |
Dussert, Stéphane |
title |
Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
title_short |
Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
title_full |
Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
title_fullStr |
Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
title_full_unstemmed |
Comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
title_sort |
comparative transcriptome analysis of three oil palm fruit and seed tissues that differ in oil content and fatty acid composition |
url |
http://agritrop.cirad.fr/569763/ http://agritrop.cirad.fr/569763/1/document_569763.pdf |
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